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OfflineNightPuma1
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Agar question Please look
    #26713513 - 06/02/20 09:11 AM (3 years, 8 months ago)

So I transferred a wedge of rhizomorphic growth to this petri dish. If you look at the picture in the center, you can see the growth there is kind of "fluffy" and not rhizomorphic (yet all the growth around it is very rhizomorphic). But also I've noticed that other times in the past the initial growth on a petri dish after transfer tends to be a little fluffy like the mycelium is trying to Colonize the air.

Either way I don't think this is infected, but I am not sure if I isolated rhizomorphic growth or not (the wedge I took wasn't even close to fluffy growth yet here it is).

So which is it? Thank you!




Edited by NightPuma1 (06/02/20 09:12 AM)


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OfflineDigDug
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Re: Agar question Please look [Re: NightPuma1]
    #26713521 - 06/02/20 09:15 AM (3 years, 8 months ago)

That looks rhizomorphic.  Give it another day or two and it will really start looking nice.


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OfflineNightPuma1
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Re: Agar question Please look [Re: DigDug]
    #26713531 - 06/02/20 09:21 AM (3 years, 8 months ago)

And what do we say about the fluffy growth in the dead center?


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OfflineDigDug
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Re: Agar question Please look [Re: NightPuma1]
    #26713536 - 06/02/20 09:24 AM (3 years, 8 months ago)

That always happens.  The mycelium tends to colonize the media from the transfer wedge before moving onto the new agar.  Maybe someone else here can offer their 2 cents with a more scientific explanation, but all of my transferred cultures have a fluffy center.


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Offlinesonoramo
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Re: Agar question Please look [Re: DigDug]
    #26713596 - 06/02/20 09:49 AM (3 years, 8 months ago)

This isn't particularly "scientific" but I've seen that mycelium moves from agar to agar or agar and especially agar to grain the same way I do when I go swimming in cool water on a hot day. At first, I'm hesitant and I want to stay with whatever I have. Once I get all-in, it feels great and I'm ready to swim away from shore.  :wineswim:


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OfflineNightPuma1
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Re: Agar question Please look [Re: sonoramo]
    #26719199 - 06/04/20 01:16 PM (3 years, 8 months ago)

So would you say this happens to you as well?
When you transfer a clean piece of rhizomorphic growth to a petri dish, at the transfer point you get some fuzzy growth that doesn't really look rhizomorphic?


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