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Offlinenoobify
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Registered: 06/03/20
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How to get plates to "ryz"
    #26718947 - 06/04/20 11:32 AM (3 years, 7 months ago)

First, hello everyone! Looong time lurker as I never really felt competent enough and frankly using search has netted almost every answer I'm looking for.

Quick background, I started off on BRF 5 years ago, moved to agar (Tiger Drop TEK) for bulk just to see if I could. Now I'm getting into cloning with agar and I'm looking for that famous ryz growth. I have done transfers up to 5 times trying to grab that elusive clean genetic but I almost always end up halfway there.

The results are still good, and they work but now I'm looking for that next little success story. I've seen so many pics of complete rhizome plates and I'm jealous. How do I need to go about getting my plates to that point? I'm using LME agar and regular cubes. Dipping my foot into APE, and I'm still super excited by the fuzzy offspring!

I take all of my transfers from the edges of the plate where the ryz seems to be forming but my understanding beyond that point is lacking.

Not mine, but like this (Hhnnng):


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OfflineA.k.aM
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Registered: 10/27/19
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Re: How to get plates to "ryz" [Re: noobify]
    #26718990 - 06/04/20 11:49 AM (3 years, 7 months ago)

Rhizo is mostly just an aesthetic thing.

But using low nute plates and pouring them thinner than normal almost always creates rhizo ime.


Also some varieties are just super tomentose, for example rusty whyte. I also had a lot of fluff with aa+ which makes sense since they’re related.

Anyway for 450ml try like 4g lme.


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LAGM2020


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: How to get plates to "ryz" [Re: noobify]
    #26718996 - 06/04/20 11:50 AM (3 years, 7 months ago)

I would do spore to agar again if you’re not getting the growth patterns you are looking for.
I usually run 15-20 spores plates of a variety to nearly ensure I’ll get a rhizo growth pattern. Just because it’s rhizo doesn’t mean it’s better than tomentose.


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OfflineSockadin
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Re: How to get plates to "ryz" [Re: Strainsfordaze]
    #26719008 - 06/04/20 11:54 AM (3 years, 7 months ago)

This last statement is very true. Rhizomorphic growth is easier to see contamination because it is organized. But tomatose growth is perfectly fine as well. There is no defined difference in terms of fruiting my yields. RW is a good example of that. It is almost always tomatose.


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Offlinenoobify
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Re: How to get plates to "ryz" [Re: Sockadin]
    #26719423 - 06/04/20 03:15 PM (3 years, 7 months ago)

That's good to know, I appreciate the input. Now if these plates will take off faster... but I'll be patient whether I want to or not.


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OfflineMH5109
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Re: How to get plates to "ryz" [Re: noobify]
    #26719431 - 06/04/20 03:22 PM (3 years, 7 months ago)

My aa plates are almost always tom plates too.


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