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OfflineTerb
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Registered: 08/31/14
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Print to Agar -- Growth or Contam?
    #26718565 - 06/04/20 07:51 AM (3 years, 7 months ago)

Pics down below. Same dish just with a different background so it makes it easier to see.

Done in a SAB and just scraped a bunch of spores onto the dish with a knife.



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InvisibleDephect
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Registered: 08/10/07
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Re: Print to Agar -- Growth or Contam? [Re: Terb]
    #26718671 - 06/04/20 09:07 AM (3 years, 7 months ago)

Id say it looks like myc, I would make a transfer soon.

Are those black specs spores?


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OfflineTerb
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Re: Print to Agar -- Growth or Contam? [Re: Terb]
    #26722307 - 06/05/20 04:41 PM (3 years, 7 months ago)

They are spores, yes. I did a transfer so we'll see what happens!


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InvisibleLadysKnight
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Re: Print to Agar -- Growth or Contam? [Re: Terb]
    #26722324 - 06/05/20 04:50 PM (3 years, 7 months ago)

That's the stuff, congrats!
Nice looking culture too.
Goddamn that's a lot of spores.
Odd you didn't get more germination.


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InvisibleRoger Clemency
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Re: Print to Agar -- Growth or Contam? [Re: LadysKnight]
    #26722363 - 06/05/20 05:13 PM (3 years, 7 months ago)

When you germinate spores you can get away with using a lot less and it is better all around. You keep your print in good shape and you can use it many many times, and youll have less chance of any bad spores being mixed in with the good ones on the agar.

Flame your inoculation loop or blade, dip it into the receiving plate to cool it and get a little sticky coat of agar, rub a small area of the print, then swipe the spores in a line or zigzag or however you like. A little spreading is good.

You can also just let the loop cool on its own and then scrape spores from the print. you wont get as many on there but for me it's always been enough and there's no chance of leaving any agar on the print itself.

That does look like cube myc but it's really weird the way it germinated in that spot and no others. something seems strange but just wait to see how the transfer looks.


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