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OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
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Registered: 05/12/07
Posts: 69,333
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Re: Could One Make A Liquid Culture With Mycelium Rather Then Spores? [Re: Typerwritermonky]
    #26715815 - 06/03/20 05:32 AM (3 years, 7 months ago)

I would recommend transfering mycelium to agar, cleaning it up and then let it grow then add pieces of colonized agar to a liquid nutrient solution to make a Liquid Culture.


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OfflineIzzamagic
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Registered: 04/20/20
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Last seen: 1 year, 8 months
Re: Could One Make A Liquid Culture With Mycelium Rather Then Spores? [Re: Stipe-n Cap] * 1
    #26715880 - 06/03/20 06:05 AM (3 years, 7 months ago)

Quote:

p9hu7 said:
Why is it that every noob mentions bod and "his"teks almost immediately.

Many TCs use LC and even if they didn't it's irrelevant. People do what they prefer personally like anyone else. There's definitely something to be said for the 5-7 day colonization with LC as apposed to 2-3 weeks with a2g.

If you'd like to make LC go here.
https://www.shroomery.org/forums/showflat.php/Number/26022486

Either make LC or make LI, no need to combine the two.
Spores to LC is el retardo...




How many times have you changed your mind just in this thread? Calm down


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Offlinepoisoned
untitled
I'm a teapot User Gallery


Registered: 04/17/13
Posts: 1,738
Loc: Yurop
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Re: Could One Make A Liquid Culture With Mycelium Rather Then Spores? [Re: Izzamagic] * 1
    #26715887 - 06/03/20 06:09 AM (3 years, 7 months ago)

Inoculating with spores is a big no-no.


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How I do glass dishes


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InvisibleRoger Clemency
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Registered: 03/23/20
Posts: 2,005
Re: Could One Make A Liquid Culture With Mycelium Rather Then Spores? [Re: poisoned] * 1
    #26715933 - 06/03/20 06:43 AM (3 years, 7 months ago)

Cuz Bodwin collected the good teks all in one spot, probably added some of his own twists, and made a flow chart. Everyone loves a flow chart bro. Now all the info you could ever need to grow tons of fun is right there and it’s so easy to just hit someone with a link to that and tell them to read read so it’s become like cube growing 101 mandatory no-ledge. Then the salty or standoffish attitude creates the barrier of mystique and draws more people in, seeking app. I watch all this stuff with fascination. People watching in the digital age, tinted by certain shared proclivities. A grand adventure we’re all on with this crazy spinning and swirling rock whose ephemeral shield protects us from the chaos. Proselytize


--------------------
Sour grapes, sweet revenge
Heaven starts right where hell ends


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InvisibleLadysKnight
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Posts: 1,670
Re: Could One Make A Liquid Culture With Mycelium Rather Then Spores? [Re: Roger Clemency]
    #26715987 - 06/03/20 07:22 AM (3 years, 7 months ago)

I enjoy your observations. Are you enlightened? Cuz you sound enlightened. Can I follow you around? Maybe an address for donations?


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InvisibleModularMind
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Re: Could One Make A Liquid Culture With Mycelium Rather Then Spores? [Re: LadysKnight]
    #26716002 - 06/03/20 07:30 AM (3 years, 7 months ago)

Quote:

LadysKnight said:
I enjoy your observations. Are you enlightened? Cuz you sound enlightened. Can I follow you around? Maybe an address for donations?




He’s in jail.
Send bail money.


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InvisibleLadysKnight
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Posts: 1,670
Re: Could One Make A Liquid Culture With Mycelium Rather Then Spores? [Re: ModularMind] * 1
    #26716013 - 06/03/20 07:34 AM (3 years, 7 months ago)

Quote:

ModularMind said:
Quote:

LadysKnight said:
I enjoy your observations. Are you enlightened? Cuz you sound enlightened. Can I follow you around? Maybe an address for donations?




He’s in jail.
Send bail money.




Directions unclear.
Sent hookers and blow.


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InvisibleStipe-n CapMDiscord
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Re: Could One Make A Liquid Culture With Mycelium Rather Then Spores? [Re: Izzamagic]
    #26716030 - 06/03/20 07:41 AM (3 years, 7 months ago)

Quote:

Izzamagic said:
Quote:

p9hu7 said:
Why is it that every noob mentions bod and "his"teks almost immediately.

Many TCs use LC and even if they didn't it's irrelevant. People do what they prefer personally like anyone else. There's definitely something to be said for the 5-7 day colonization with LC as apposed to 2-3 weeks with a2g.

If you'd like to make LC go here.
https://www.shroomery.org/forums/showflat.php/Number/26022486

Either make LC or make LI, no need to combine the two.
Spores to LC is el retardo...




How many times have you changed your mind just in this thread? Calm down




Not once.

Quote:

Could One Make A Liquid Culture With Mycelium Rather Then Spores? 




Mycelium is used to make LC not spores

Quote:

could you not inject a solution of mycelium and sterelized water




Yes if it's a biopsy syringe to LC, or LC to LC, no if its LI because just make LC.

Pretty easy stuff.

Quote:

calm down








Edited by Stipe-n Cap (06/03/20 08:27 AM)


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OfflineDharmaForKarma
Tub monkey
Male

Registered: 04/24/20
Posts: 565
Last seen: 1 day, 12 hours
Re: Could One Make A Liquid Culture With Mycelium Rather Then Spores? [Re: LadysKnight]
    #26716031 - 06/03/20 07:42 AM (3 years, 7 months ago)

Quote:

LadysKnight said:
Directions unclear.
Sent hookers and blow.




Now that is a magic 8 ball.


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OfflineTyperwritermonky
shboop a doop a doop


Registered: 01/19/12
Posts: 5,375
Loc: Mrs. Brown's Teahouse
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Re: Could One Make A Liquid Culture With Mycelium Rather Then Spores? [Re: DharmaForKarma]
    #26716870 - 06/03/20 01:44 PM (3 years, 7 months ago)

Thanks yall for all the answers!  The liquid culture TEKs I found ALL had them being created by injecting a spore syringe into the sterilized sugar broth jar where they would prosper, so I was just thinking maybe it could be done with mycelium instead.

As well, this would be done with cyanescens (wavy caps) which are super hardy and tend to beat out other competition well.

Basically I have a jar of cyanescens mycelium with sterilized alder bark thats finally taken its foothold and is really starting to spread.  But the jar is too big and I am thinking of what I want to do with it to get things done in a timely manner.

So my idea is to get the mycelium onto agar and then use that to make a LC to grow directly from and inoculate with, and then for the rest of it put it into a smaller jar with better food and compost to make a fully colonized jar to then make outdoor beds with.

thanks yall for putting up with my ignorance!


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