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gecata
New Mycologist



Registered: 05/05/20
Posts: 20
Loc: Bulgaria
Last seen: 3 years, 6 months
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PF Tek - First Jars [CONTAM]
#26713083 - 06/02/20 05:53 AM (3 years, 7 months ago) |
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Hi guys. ----- THIS IS A SHORT BACKGROUND, YOU CAN SKIP TO THE QUESTION/PROBLEM ------- I recently loaded my first 7 jars (6 240ml jars; 1 500ml) with Verm, BRF and water 2:1:1. I steam-sterilized them for about 2 hours and let them cool overnight without removing the lid. I then made a spore syringe using part of a spore print I had in the fridge (spore prints on foil that was sealed in a zip-lock bag). This syringe process was done in a SAB, using gloves, wiping everything with 70% alc and flame sterilizing. I then flame sterilized the syringe before each next jar and waited for 10 seconds until the needle wasn't hot anymore. I injected in 3 holes - one in the middle, and two on the sides next to the glass. So I took the SAB and loaded all the jars inside and left them in my room which is hotter than the rest of the rooms. The jars have indirect sunlight and the SAB is transparent. I've closed the SAB hand holes with a lid from another container so that gas can escape but nothing can fall through the holes. ------- ------------PROBLEM ------------------------ So I loaded them on Friday (29/05) and now it's Tuesday (02/06) and I finally started seeing some fuzzy, web-like specs in some of the jars. One of them has a white web-like growth (its still super tiny) the rest don't have anything that I can notice yet with the exception of two which have green web-like growth (super small too) ----------------- QUESTION -------------------- So I'm guessing that's Trich. What should I do, should I throw them right away? Or should I wait a bit more to make sure that's the case? Is there any possibility they can contaminate the other jars?
-------------------- Banning psychedelics might be one of the biggest forms of oppression on human consciousness.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: PF Tek - First Jars [CONTAM] [Re: gecata]
#26713102 - 06/02/20 06:05 AM (3 years, 7 months ago) |
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If they are green it is probably aspergillus or another green mold like penicillin.
Either way toss them. Blame the syringe.
Spores syringes are never clean no matter how clean you are with them.
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gecata
New Mycologist



Registered: 05/05/20
Posts: 20
Loc: Bulgaria
Last seen: 3 years, 6 months
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Re: PF Tek - First Jars [CONTAM] [Re: Sockadin]
#26713111 - 06/02/20 06:12 AM (3 years, 7 months ago) |
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A sad moment, but I guess it's part of the learning process... Do I throw the whole batch or just the two with the green growth?
-------------------- Banning psychedelics might be one of the biggest forms of oppression on human consciousness.
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DescensionWayne
Mind Descender

Registered: 10/10/19
Posts: 14
Loc: Mo
Last seen: 3 years, 4 months
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Re: PF Tek - First Jars [CONTAM] [Re: gecata]
#26713223 - 06/02/20 07:22 AM (3 years, 7 months ago) |
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I keep my failures close by to stare at me as a reminder.
I would keep the ones that that seem fine, they may turn out well. My first time with pf tek gave me 3 succeses out of 12 jars.
-------------------- If you are going to do something stupid, be smart about it.
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gecata
New Mycologist



Registered: 05/05/20
Posts: 20
Loc: Bulgaria
Last seen: 3 years, 6 months
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Where and how do you guys dispose of such contaminated cakes? Should I just throw them outside in the dirt? Or straight to the trash bin (outside ofc)
-------------------- Banning psychedelics might be one of the biggest forms of oppression on human consciousness.
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gecata
New Mycologist



Registered: 05/05/20
Posts: 20
Loc: Bulgaria
Last seen: 3 years, 6 months
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Re: PF Tek - First Jars [CONTAM] [Re: gecata]
#26713618 - 06/02/20 10:06 AM (3 years, 7 months ago) |
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Oh I have another question. Since I added the spores to two sides and the center, how do I know that there isn't any co tam growth in the center? Are there any signs I should be looking out for? So far all of the 5 remaining jars seem to be looking good. The all hive nice small white patches, no greens.
-------------------- Banning psychedelics might be one of the biggest forms of oppression on human consciousness.
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lefty24
Stranger
Registered: 06/02/20
Posts: 72
Last seen: 3 years, 6 months
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Re: PF Tek - First Jars [CONTAM] [Re: gecata]
#26713634 - 06/02/20 10:12 AM (3 years, 7 months ago) |
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Steam at boiling temp won't sterilize. It's 212 degrees. Get a pressure cooker. 15 PSI 90 minutes you won't contam at least not for that reason.
Edited by lefty24 (06/02/20 10:14 AM)
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gecata
New Mycologist



Registered: 05/05/20
Posts: 20
Loc: Bulgaria
Last seen: 3 years, 6 months
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Re: PF Tek - First Jars [CONTAM] [Re: lefty24]
#26713681 - 06/02/20 10:34 AM (3 years, 7 months ago) |
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Thanks for the suggestion. I will invest in a pressure cooker in the near future. Do you have a suggestion on how I can monitor for contamination deep inside the cake? Is it going to show up once the cakes are fully colonized? Would it be a good idea to cut the cakes in 2 with a knife once they are colonized?
-------------------- Banning psychedelics might be one of the biggest forms of oppression on human consciousness.
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DescensionWayne
Mind Descender

Registered: 10/10/19
Posts: 14
Loc: Mo
Last seen: 3 years, 4 months
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Re: PF Tek - First Jars [CONTAM] [Re: gecata]
#26713960 - 06/02/20 12:31 PM (3 years, 7 months ago) |
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I wouldn't, but you *could* break them up and spawn them with substrate to a tub. I don't like the inoculate the center for that exact reason.
-------------------- If you are going to do something stupid, be smart about it.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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PF is contamination prone if your molds came from the syringe. So a PC still won't fix the issue if your syringes (which are always dirty) have contamination in them. The only way to tell is with agar.
But for simplicity. Just keep going.
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gecata
New Mycologist



Registered: 05/05/20
Posts: 20
Loc: Bulgaria
Last seen: 3 years, 6 months
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Re: PF Tek - First Jars [CONTAM] [Re: Sockadin]
#26715017 - 06/02/20 08:00 PM (3 years, 7 months ago) |
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So I threw the contaminated ones and I'm left with 5 healthy-looking ones (at least so far). The white mycelium has started growing quite fast and is looking clean and good. My thinking was I'll make holes on the sides and in the center for faster inoculation with the idea that if there is any mold (coming from the syringe) I will see it on the side growth. Now that I've already done what I did, is there any danger if I grow the seemingly healthy cakes (once they are done) while there is an unhealthy core? Like what if the core has mold that I can't see and I grow the cakes, is there any danger in doing so?
-------------------- Banning psychedelics might be one of the biggest forms of oppression on human consciousness.
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