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Offlineausmedic
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Registered: 05/11/20
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Last seen: 3 years, 4 months
Oyster clone pinning on agar before mycelium growth?!
    #26712266 - 06/01/20 07:39 PM (3 years, 7 months ago)

Hey guys,
I'm not sure what's going on because I'm an absolute beginner. Trying to practice with a grocery store bought oyster mushroom.
I planted a section of the stem as carefully as I could into 2% MEA with gentamicin which I had made using a no pour tek.
The mycelium did start to grow a little bit out from the clone but almost immediately there was some vertical growth which has now developed into a very clear cluster of pins. There has hardly been a 1cm of horizontal growth. Any idea what's going on? See pics.


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OfflineMachiavelliavore
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Registered: 12/08/14
Posts: 3,038
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Last seen: 3 months, 19 days
Re: Oyster clone pinning on agar before mycelium growth?! [Re: ausmedic]
    #26712299 - 06/01/20 07:50 PM (3 years, 7 months ago)

Maybe some sneaky bacteria there, but with those atibiotics idk.  Personally I'd just pull the pin off and stick that on a new plate since it's all new growth.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineA.k.aM
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Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
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Re: Oyster clone pinning on agar before mycelium growth?! [Re: Machiavelliavore]
    #26713327 - 06/02/20 08:02 AM (3 years, 7 months ago)

Yeah I agree with transferring the pin.

Maybe it either doesn’t like your agar or you got lucky and just have the easiest pinning culture of all time.


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LAGM2020


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