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OfflineSVT_OP
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Registered: 05/11/20
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Help with Contam ID on agar
    #26711769 - 06/01/20 03:49 PM (3 years, 7 months ago)

Hey all, first post here. Seeking advice on what appears to be contam on these agar plates (apologies for the excess condensation). I inoculated them 5/23 using a GT MSS. Initially they appeared completely white in appearance, but began taking on a pink hue as the days went by. My initial thoughts were a contaminated syringe, but this same syringe was used to inoculate spawn jars a few days before. The mycelium on the spawn jars show no signs of contam and are progressing as expected.

If it is contam, I’m thinking it was introduced into these samples during the actual inoculation process. In any case should I be worried about my spawn jars going bad with this stuff over the next few weeks? Also if any could identify the contam? Thanks!







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InvisibleRoger Clemency
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Registered: 03/23/20
Posts: 2,005
Re: Help with Contam ID on agar [Re: SVT_OP]
    #26711784 - 06/01/20 03:59 PM (3 years, 7 months ago)

Yoo that doesn't look good brochacho. If every spot that started out as a white spot eventually turned pink then you've got a pretty contaminated syringe i'd say. Whatever is in the jar will most likely start turning pink once its a little further along.

Do you have a pic of the jars? Are they BRF or grizzains?


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InvisibleMethodicalMystical
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Registered: 07/16/17
Posts: 247
Loc: PNW
Re: Help with Contam ID on agar [Re: Roger Clemency]
    #26711809 - 06/01/20 04:08 PM (3 years, 7 months ago)

:whathesaid: Don't count on those jars reaching full colonization and remaining healthy, especially if by "spawn" you mean grains. Noccing them with spores is a crapshoot even in the best conditions.

Most any mold with a pink/purple color is bad news, so your best course of action may be to toss everything that has it and get a new syringe.


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