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Offlinemakomeyer
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Cold shock bad idea? PF cakes blueing and not fruiting after 1wk
    #26711393 - 06/01/20 12:23 PM (3 years, 7 months ago)

Hey folks,

Hoping someone can help me troubleshoot a couple issues - here's the background:

I inoculated 9 half-pint BRF/verm jars and all were fully colonized around 5/11-13 except for two. In the midst of this COVID situation I had to leave town and ended up staying longer than originally planned so on 5/18 I had a friend go to my apartment and put the first 7 to colonize in the fridge since I wouldn't be home to birth/fruit them for another week. The other 2 "late bloomers" appeared to be just reaching full colonization at that point so considering my travel plans, I told him to leave those guys out thinking they would be ready to come out right when I got home.

When i got home on 5/25 one week ago, the two late bloomers had some invitro pinning, one with a couple decent sized ones. So, I birthed all 9 jars, did a room temp dunk and roll and made sure to be extra careful not to harm or even touch the pins. Placed them all in my SGFC the following day, 5/26.

Fast forward to today and only two cakes are pinning, the rest are blueing a bit although there is at least some new mycelium growth but they haven't really colonized the verm casing layer much at all. The one cake in the middle of the terrarium with visible fruits is the one that had the big invitro pins, but those fruits appear to have stalled (should i pick them off?). My assumption is the one other pinning cake to the left of it is the other one that was not put in the fridge at all.

Another thing I should note: I hate to admit this but I left my SGFC in direct sunlight a couple times and the temp was 85-95 degrees for up to 4 hours. I also left the top off yesterday for a couple hours with the fan on them after misting (meant to try this for only a few minutes cause my arms were tired lol, but i got distracted). I know, I know, I apologized to them profusely and hopefully they forgive me. Yes, I have some short term memory loss problems! All of this could have something to do with the blueish hue today I suppose.

Did I screw this up by putting them in the fridge? Or am I just not being patient enough? I'm also struggling to keep my temps down and still get some sunlight but I think I'll just keep them off the window sill during the day. Overnight it seems to be fine, not going any lower than 68. See attached images.

Any advice is appreciated, thank you!



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InvisibleRoger Clemency
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Re: Cold shock bad idea? PF cakes blueing and not fruiting after 1wk [Re: makomeyer]
    #26711561 - 06/01/20 01:45 PM (3 years, 7 months ago)

Putting them in the fridge is a way to temporarily postpone fruiting, though the old idea of a “cold  shock” for cubes doesn’t actually help. But maybe your fridge was too cold and semi froze some of the mycelium.

There are two aborted mushrooms on that cake that you should take off, the other two are looking nice. For the other cakes you just have to wait and see man, they haven’t been in happy conditions for very long yet. No need to fan especially with a proper SGFC. They get mad air all the time unless it’s stuffed in a closet or something. Mist whenever they start looking dry. The more you mess with them the less happy they are most of the time lol. Just let em roll


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Offlinemakomeyer
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Re: Cold shock bad idea? PF cakes blueing and not fruiting after 1wk [Re: Roger Clemency]
    #26711959 - 06/01/20 05:36 PM (3 years, 7 months ago)

Thanks Roger! Fortunately I can almost guarantee that they did not freeze in the fridge - it's on the least cold setting and they were stored on the door which gets chilled the least.

I didn't pick off the aborts thinking I didn't want to mess with the cakes any more than I needed to (especially the one on the right whose whole stem is attached to the cake) and also thought the myc might even colonize that tissue and it would just get incorporated into the cake. But, I'll pick off the aborts. Question is - should I dry and eat them?

I read several threads/teks that advocate for a little bit of manual fanning after each misting to spike evaporation to trigger pins, but that was also a few years ago last time I did this and really read up on the forums. Is that no longer a preferred technique?


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Offlinemakomeyer
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Re: Cold shock bad idea? PF cakes blueing and not fruiting after 1wk [Re: makomeyer]
    #26716402 - 06/03/20 10:32 AM (3 years, 7 months ago)

Update: The cake with the aborts that I picked off produced two medium sized and two small fruits for a total of only about 30g wet. There were a few small pins that aborted that I picked off as well. The fruits were blue at the base and seemed to break their veil earlier than I remember from previous experience. Also I may need stronger or more direct artificial light because they were growing in all kinds of directions. Should I just call it the first flush for this cake, dunk and roll, and hope for better results on the next flush? Or give it a couple days?

Two of the cakes that were refrigerated are starting to pin, but one of them looks about to abort. Still praying for them! A little frustrated but trying to stay hopeful...any tips or encouragement is appreciated!

Photos:
1 - aborting pin on refrigerated cake
2 - seemingly healthy pin on different refrigerated cake
3 - non-refrigerated cake first flush. You can see the blue at the bottom.
4 - Same cake as above after the two bigger fruits picked. Growing direction inconsistent, as well as inconsistent genetics (one looks albino).
5 - small harvest from first flush of first cake to produce.




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InvisibleRoger Clemency
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Re: Cold shock bad idea? PF cakes blueing and not fruiting after 1wk [Re: makomeyer]
    #26718527 - 06/04/20 07:12 AM (3 years, 7 months ago)

Some people swear by fanning but I’ve used a SGFC before and at least in my experience it’s like fanning an outdoor plant. There’s a lot of air coming in all the time. It’s just preference I guess.

Those cakes look good man. The small harvest kinda sucks but it looks like they will put out some more. I don’t know about dunking that one since it hasn’t used too much water. They do lose water just from evap though so maybe try picking it up and if it feels noticeably light it might benefit from a few hour dunk. No need for rolling in verm again though.


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Offlinemakomeyer
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Re: Cold shock bad idea? PF cakes blueing and not fruiting after 1wk [Re: Roger Clemency]
    #26722051 - 06/05/20 02:10 PM (3 years, 7 months ago)

Roger that on the fanning - not going to bother anymore.

I dunked that cake and skipped the roll per your suggestion. What's the reasoning behind rolling after the initial dunk but not for subsequent flushes? Just wondering because that guy looks sad AF right now (see pic below).

Some of the other cakes are starting to pop off finally, but the timing of when the individual fruits reach maturity is very sporadic. I'm basically picking off one or two per day on different cakes. Because of this I'm a little unsure of when the "flush" is complete and I need to dunk again, and whether to roll again or not. What's the main deciding factor there - just moisture content and keeping them hydrated? Unless someone says otherwise I'll just go by weight / density and once they feel light I'll dunk em.



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