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OfflineheadyAF
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Registered: 06/01/20
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Agar Contamination?
    #26710975 - 06/01/20 09:04 AM (3 years, 8 months ago)

First time messing with agar, realize now I had too much moisture built up on the lids of my plates after pouring. It's been around 2 weeks since I inoculated and I'm starting to see a few things I think may be contamination but am not certain. These spots are faintly blue and a little orange when held up to the light but you can't really see them unless you hold them to the light, except for the first one. Read mixed things about blue being normal sometimes if the agar dries out? Also orange could be stressed myc releasing chemicals? Spots do not seem to be spreading and are only on the mycelium as far as i can tell. What do you all think? (Also realize now I sort of messed up by doing multiple drops on one plate)

Plate 1


Plate 2




Plate 3




Plate 4






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OfflineTrichodingus
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Registered: 05/31/20
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Re: Agar Contamination? [Re: headyAF]
    #26711100 - 06/01/20 09:58 AM (3 years, 8 months ago)

Looks like contamination to me. I'd just transfer a clean section to be certain.

Better to be certain early and not worry that everything is gonna go off the rails later!


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InvisibleRoger Clemency
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Registered: 03/23/20
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Re: Agar Contamination? [Re: Trichodingus]
    #26711174 - 06/01/20 10:27 AM (3 years, 8 months ago)

There’s really no “contamination” of agar...unless you mess up sterilizing or pouring. You put spores there to germinate so you can see what you have, hopefully just myc but other things are expected at first. You should transfer clean growth away once germination and a little growth has occurred, those are still okay to transfer from but usually the earlier the better.

The growth should get more concise and clean looking after a transfer or two.


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Heaven starts right where hell ends


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Offlinepoisoned
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Re: Agar Contamination? [Re: Roger Clemency]
    #26711446 - 06/01/20 12:51 PM (3 years, 8 months ago)

Yeah, just take some transfers from clean growth.


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How I do glass dishes


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