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Offlinedfittlesticks
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Registered: 05/14/20
Posts: 25
Last seen: 1 year, 1 month
1st attempt failure. Trying again. Need help
    #26705180 - 05/29/20 05:01 PM (3 years, 7 months ago)

this first time I used a kit with 12 jars and an incubator.  Well, I think it is time to admit defeat after 26 days and try again.  I wasn't sure if my spores were bad, or if the jars were dry so a week ago, after 19 days in and nothing growing in all 12 jars, I took 2 of the jars and under sterile conditions I managed to pour in about 4 oz of distilled water into each.  After a week they still aren't growing anything.  Yesterday I took one of the other jars that I didn't add any water to and poured out the substrate. It felt damp.  Not soaking wet, but damp, and this was after being in an incubator for 27 days where some moisture may have vaporized out.  There were no signs of growth at all, fungus or contaminate, so I am concluding that the spores I got from (vendor name omitted) were dead.  I tried 2 different strains with 2 syringes and nothing from either.  They were only $10 for a 12cc syringe so I guess you get what you pay for.

So... now I am about to make my own PFTek. I have been reading up and getting confused with contradictory points. So I Have a few questions:

Do I add water to the Brown rice flour first, and then the vermiculite, or add water to the vermiculite first and then the BRF?  Does it even matter?

Is using the brown rice flour from Bob's Red Mill good, or should I grind up brown rice and make my own fresh flour?  I already have the bag, but Mr. Psilocybin Fanaticus is saying fresher BRF is better.

I was going to use distilled water, but PF himself says to use pond or stream water since it has inorganic materials in it to nurture the mycelium.  I guess I just have to sterilize the jars really well then. Any opinions on this?

I have medium sized pressure cooker that doesn't have a gauge on it.  It is a simple one that just has a relief valve if it goes higher than 10 PSI.  How long should I cook the substrate jars for 10 psi?  30 mins?  60 mins?  I don't want to over do it and possibly dry out the jars by going too long.  I believe at 10 psi the temp should get to 240 degrees.

How much water should I put in the pressure cooker?  2 inches?  I plan on putting just tin foil on the jars and setting them on top of the lids so the glass doesn't touch the metal of the cooker.  I still have 12 jars from the kit I bought. I will then screw the lids back on after they cool.  There will be a few seconds after I remove the tin foil before screwing on the lids again where the jars will be exposed, but I don't think this should hurt to much.

I ordered a new syringe from a vendor listed on this website.  Hopefully they'll be "Reliable.".  I do still have one syringe from (crap supplier) that I may give another shot into one of the jars just to double check.

Also, should I even be bothering with these fucking jars, or is using the bag method simpler and more productive?

Okay, this is a lot of questions, but I appreciate the help.  Cheers.


Edited by dfittlesticks (05/30/20 09:03 AM)


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OfflineWinterOwned
Fighter of the Nightman
I'm a teapot User Gallery
Registered: 08/30/08
Posts: 149
Last seen: 1 year, 3 months
Re: 1st attempt failure. Trying again. Need help [Re: dfittlesticks]
    #26705240 - 05/29/20 05:21 PM (3 years, 7 months ago)

I’m pretty sure you’re not supposed to call out vendors. They only sell spores for microscopy anyway.


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Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: 1st attempt failure. Trying again. Need help [Re: dfittlesticks]
    #26705315 - 05/29/20 05:47 PM (3 years, 7 months ago)

You can just throw all the ingredients in and mix them up good.
The texture should be that of mildly wet sand.
Bob's Red mills BRF is perfect good job.
I would do a 60-90min sterilize at 10psi.
1.5-2in water should work, don't go over halfway up your jars or you will have water entering them.
I wouldn't shout out vendors here mate.
Jars are a great way to do mush cult and I use more jars than I do bags tbh.
Jars rock. widemouth 1qt are what you want. For BRF you want widemouth 1/2pints.
Jars are reliable because they will last forever if you take care of them.


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Offlinedfittlesticks
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Registered: 05/14/20
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Re: 1st attempt failure. Trying again. Need help [Re: mushhead]
    #26706724 - 05/30/20 09:09 AM (3 years, 7 months ago)

Thanks all.  I took out the spore vendor's name, although I don't see how we are supposed to recommend good vendors if we can't name them.  The next spore vendor I am going to try is advertising on this website, so they better be good.

If any of you have used jars from a kit, how long do you think they will last?  I will mix up my own pftek, but these jars I have from the kit may still be good and uncontaminated.  I am just wondering if maybe they lost some moisture after incubating for 28 days.


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Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: 1st attempt failure. Trying again. Need help [Re: dfittlesticks]
    #26706941 - 05/30/20 10:58 AM (3 years, 7 months ago)

I'd try to use em if you have expendable spore.


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OfflineBsfixit
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Registered: 10/23/19
Posts: 132
Last seen: 2 months, 18 days
Re: 1st attempt failure. Trying again. Need help [Re: mushhead]
    #26706952 - 05/30/20 11:08 AM (3 years, 7 months ago)

It is important to mix the vermiculite with the water first throughly then add the brown rice flour
If you don’t , you end up making dough


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OfflineMugnath
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Registered: 05/24/12
Posts: 733
Loc: Oregon
Last seen: 8 months, 16 days
Re: 1st attempt failure. Trying again. Need help [Re: dfittlesticks]
    #26706956 - 05/30/20 11:11 AM (3 years, 7 months ago)

Recommended methods of which order to add brf, water, and verm is due to brf clumping when in solo contact with water. Mix the brf with the verm before adding water, or mix the verm with water first to avoid the extra work of breaking up clumping brf.

When you PC your jars, ensure that you have them stacked high so they aren't sitting in water, and ensure that you have enough water that you don't dry out. About two lids stacked in height, which is also the amount of water to add (do you happen to have a Trivet for your PC?).


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OfflineSockadin
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Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
Trusted Cultivator
Re: 1st attempt failure. Trying again. Need help [Re: dfittlesticks]
    #26706984 - 05/30/20 11:32 AM (3 years, 7 months ago)

You can through a dish towel in the bottom of the pot and set your jars on that.

2 inches of water.

I like to dry mix my BRF and Vermiculite and slowly add water to prevent clumping.

Also if you lids have Inoculation holes make sure to out the on the jar over your dry vermiculite barrier and then cover in foil. If you holes were punched with a nail I like to have the depressed part up so it makes a little volcano like upwards rift to prevent water from pooling and running in those holes.

If you still have the old syringes use 1/2 and ,1/2 to test the syringe viability.

And absolutely throw out that TIT. Or any other heat mat. They are junk and will cook your spores before they can actually germinate.

If you can stand the indoor climate of your home, the spores and mycelium can aswell.

Good luck!


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