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OfflineEnkidu
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Registered: 07/09/16
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Print to agar : transfer and contam transfer
    #26704943 - 05/29/20 03:25 PM (3 years, 7 months ago)

Hey whats up



So i was hoping to get some rhizo growth coming out but thinking it wont happen

This look like myc to you?

Its real fluffy, no pretty rhizo, but i suspect it is myc growth

This is spore print to agar

Wondering if i should transfer some myc

Also have the pic of some contaminated plates

Was wondering if that early battle would impact the myc on those plates and what impact that would have growing it out. Not sure it does make a difference?

What do you guys think?


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OfflineMachiavelliavore
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Registered: 12/08/14
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Re: Print to agar : transfer and contam transfer [Re: Enkidu]
    #26705018 - 05/29/20 03:55 PM (3 years, 7 months ago)

Rhyzo growth will be determined by both genetics and agar recipe.  Often times the many strains that germinate will look fluffy at first.  I usually just look for the fastest growth, doesn't matter if it's rhyzo or tomentose.  Also if you see a lot of peeks or fluffy balls in the mycelium, sometimes that's a sign there is something nasty growing under it, so look for uniform growth.

You definitely have myc there, just transfer away from contams until clean and try not to take them with you.  If you're dealing with sporulating mold as a contaminant, transfer, then try and transfer from new growth before those mold spores germinate on your first transfer plate, usually ends up clean.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Edited by Machiavelliavore (05/29/20 03:56 PM)


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OfflineEnkidu
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Re: Print to agar : transfer and contam transfer [Re: Machiavelliavore]
    #26705031 - 05/29/20 04:01 PM (3 years, 7 months ago)

Hey thanks for the reaponse man

Maybe ill transfer the top 2 far left plates and see if i cant get some decent looking myc from both the PEU and RW

Its been real hot recently, upper 80s, so thought that might be part of the reason the myc was so puffy


--------------------
Within You , Without You


:mushroom2::levitate::mushroom2:


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OfflineBobbit
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Re: Print to agar : transfer and contam transfer [Re: Enkidu]
    #26705120 - 05/29/20 04:38 PM (3 years, 7 months ago)

Plenty of healthy myc there. . . Well done. . .

It looks like you are wapping the whole plate in cling wrap?

Have you tried making a ‘mini roll’ by cutting the end off your roll with a super sharp blade?


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OfflineEnkidu
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Registered: 07/09/16
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Re: Print to agar : transfer and contam transfer [Re: Bobbit]
    #26705389 - 05/29/20 06:18 PM (3 years, 7 months ago)

Hey thanks man

Yeah i was wondering if they might be clean enough to use to innoc some grain

But maybe im being a bit hasty

Got plenty of time right now anyhow

Yeah i wrapped them with about 2 in of wrap, maybe went a bit heavy

Had a hell of a time trying to get all the way through to the cardboard roll, if i made it, wasnt able to get through it, but oh well :shrug:


--------------------
Within You , Without You


:mushroom2::levitate::mushroom2:


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OfflineMachiavelliavore
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Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
Re: Print to agar : transfer and contam transfer [Re: Enkidu]
    #26705484 - 05/29/20 07:02 PM (3 years, 7 months ago)

Picture quality isn't really good enough to say if they are clean enough to noc, my guess is something in the third picture is.  I tend to distrust that sort of crater type thing in the center you got in the far right plate of pic 3.
Obv pic one has some dirty shit in there, though I think you could get clean transfers out of all of them in 1-2 generations.

Top center, bottom left, and center looks like the best 3 plates in pic 3 to me, but again, pic quality is not there to say with any certainty.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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