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Offlinemrfinkle
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How long between sterilization and inoculation? * 1
    #26703040 - 05/29/20 12:04 AM (3 years, 7 months ago)

Hello friends! I’m doing PF Tek and I was wondering how long I should wait between sterilization and inoculation. Everything I read says to inoculate immediately to avoid contamination. But wouldn’t it be too  hot for the spores to survive? Can I wait for an hour to let it cool down or will that risk contamination?


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OfflineMugnath
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Re: How long between sterilization and inoculation? [Re: mrfinkle] * 1
    #26703053 - 05/29/20 12:17 AM (3 years, 7 months ago)

The jars, leave them to cool overnight.


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Offlineherbnmyco
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Re: How long between sterilization and inoculation? [Re: Mugnath]
    #26703055 - 05/29/20 12:20 AM (3 years, 7 months ago)

Just wait till they're cool. Waiting a few day won't hurt either but the sooner the better


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OfflineLogicaL ChaosM
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Re: How long between sterilization and inoculation? [Re: mrfinkle]
    #26703071 - 05/29/20 12:33 AM (3 years, 7 months ago)

Around 8 hours is ideal. U want the jars room temp to prevent cooking your spores (ive done it, not fun).


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Offlinemrfinkle
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Re: How long between sterilization and inoculation? [Re: LogicaL Chaos]
    #26703093 - 05/29/20 12:50 AM (3 years, 7 months ago)

Alright thanks! Now what IF I already inoculated when it was too warm?😂 Can I just inoculated again once cooled down?


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OfflineLogicaL ChaosM
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Re: How long between sterilization and inoculation? [Re: mrfinkle]
    #26703099 - 05/29/20 12:54 AM (3 years, 7 months ago)

Umm, sure.

How many hours did u wait till u injected the jars?


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Offlinemrfinkle
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Re: How long between sterilization and inoculation? [Re: LogicaL Chaos]
    #26703106 - 05/29/20 12:57 AM (3 years, 7 months ago)

About 4 hours, it had cooled down room temperature by then!


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OfflineLogicaL ChaosM
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Re: How long between sterilization and inoculation? [Re: mrfinkle]
    #26703112 - 05/29/20 12:59 AM (3 years, 7 months ago)

U might be OK. I would hold off on a second injection. As long as the jar galss was cool to the touch, u should be OK.


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