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TrippyMomp



Registered: 04/16/20
Posts: 185
Last seen: 1 year, 4 months
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WBS jars questions/ AGAR SOS
#26702551 - 05/28/20 07:29 PM (3 years, 7 months ago) |
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so I wanna get my WBS started well actually already started they're cooking in the PC right now following the 24 hr soak and rinse and then boil soak. Can I take a culture from one of my mushrooms growing on my Pf cake and drop it into one of the jars ? could I do this with a few then pick a master jar then do G2G from that ? Have my shoeboxes filling with myc right now but most are GT. the one cake with the big caps rn is PT. I'm worried my other two syringes my Ecuador and FIJI got sterilized through the mail process the EC show bacteria at least on agar but the Fiji I can see spores on the agar multiple but nothings happened on multiple plates not just one. I'm having trouble with these two on agar any ideas ? I've tried both the one drop in the middle technique as well as a normal inoculation zig zag with a loop and flame. I want diversity and untimely don't want to buy any more spores when I have 4 types at home 2 which are not cooperating. All this learning has my green thumb going fashow.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: WBS jars questions/ AGAR SOS [Re: TrippyMomp]
#26702572 - 05/28/20 07:37 PM (3 years, 7 months ago) |
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Ok. This is really hard to follow for an old drunkard like me.
If you have fully colonized PF jars and you wake a piece of it when you birth them. Yes you can inoculate your WBS with it. I advise taking it to agar and cleaning it up. But you seem like you are already ready to go jar to grain.
Also I don't understand the mail question.
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TrippyMomp



Registered: 04/16/20
Posts: 185
Last seen: 1 year, 4 months
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Re: WBS jars questions/ AGAR SOS [Re: Sockadin]
#26702701 - 05/28/20 08:48 PM (3 years, 7 months ago) |
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Quote:
Sockadin said: Ok. This is really hard to follow for an old drunkard like me.
If you have fully colonized PF jars and you wake a piece of it when you birth them. Yes you can inoculate your WBS with it. I advise taking it to agar and cleaning it up. But you seem like you are already ready to go jar to grain.
Also I don't understand the mail question.
I'm a scatter brain so I apologize its hard for me to organize my thoughts sometimes. All of my cakes are now "dirty" tho because they've all been broken up and mixed with coir other than one cake that has failed to fully colonize also is a taller style 8oz jar. I have mycelium on agar I just cannot get the other syringe strains to germinate. For example my first 5 pf cakes I used pink thai 2 of the 5 colonize and one of those is that one that hasnt fully. I then inoculated 10 Golden teachers all of those have colonized well.and been broken up and usee as spawn in coir. All my pt shows on agar is bacteria and my Fiji and Ecuador both seem to only show bacteria too actually the fji doesnr show anything on the agar and I see lots of bacteria and no myc on the Ecuador agar. The Fuji I'm afraid sat in my mail box to long or maybe wasnt rehydrated properly? Is that possonlenin a syringe I see a ton of spores but I live in a hot place so it could have been a week before I noticed them in my mail box they sent me those ones for free and didnt tell me lol. So I just dont know how I can further trouble shoot these syringes I've tried like 5 plates now
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TrippyMomp



Registered: 04/16/20
Posts: 185
Last seen: 1 year, 4 months
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Re: WBS jars questions/ AGAR SOS [Re: TrippyMomp]
#26702770 - 05/28/20 09:13 PM (3 years, 7 months ago) |
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My gt syringe is the only one that shows myc on the agar.
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
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Loc: The Sporetorn States
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Re: WBS jars questions/ AGAR SOS [Re: TrippyMomp]
#26702775 - 05/28/20 09:15 PM (3 years, 7 months ago) |
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If you're talking about mushroom tissue direct to grain it can work but it isn't advisable. I tried it when I started, did not end well and wasted a shitton of time. If you're talking about cloning a fruit on agar, then going a2g from there after you have a clean culture, that would be the way to go. As for the dirty syringes you can't get to go on agar, try dropping to like 1/4 cup grain, transfer a grain from a healthy looking area area to agar can be a way forward. Only use a few drops. You could also try that with a small BRF cake sub.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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TrippyMomp



Registered: 04/16/20
Posts: 185
Last seen: 1 year, 4 months
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Quote:
Machiavelliavore said: If you're talking about mushroom tissue direct to grain it can work but it isn't advisable. I tried it when I started, did not end well and wasted a shitton of time. If you're talking about cloning a fruit on agar, then going a2g from there after you have a clean culture, that would be the way to go. As for the dirty syringes you can't get to go on agar, try dropping to like 1/4 cup grain, transfer a grain from a healthy looking area area to agar can be a way forward. Only use a few drops. You could also try that with a small BRF cake sub.
That was partially what I was thinking of doing. I have like 9, 8 oz grain jars 3/4 full in the pc right now and 2 pint ones. If the spores didnt work on agar what makes them more likely on brf or grain ? Just curious!
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
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Re: WBS jars questions/ AGAR SOS [Re: TrippyMomp]
#26702856 - 05/28/20 10:09 PM (3 years, 7 months ago) |
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If you have pooling water (super common on no-pours,) or excess spore solution, and a bacterial contaminant in that spore solution, the bacteria will effect the vast majority of the surface area of the plate before your spores germinate and effectively smother them. This will also occur at the bottom of the grain jar or cake if you use enough spore solution, but at the top, the excess water drains down or is absorbed, giving a much better chance for your mycelium to get rolling. You may have bacteria in the grain core, but it will likely be surrounded by mycelium, so when you put it on your plate, living mycelium gets the first chance to jump onto the agar.
I really don't suggest tissue to grain, almost impossible to tell if you're gonna waste a ton of time with it.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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TrippyMomp



Registered: 04/16/20
Posts: 185
Last seen: 1 year, 4 months
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Quote:
Machiavelliavore said: If you have pooling water (super common on no-pours,) or excess spore solution, and a bacterial contaminant in that spore solution, the bacteria will effect the vast majority of the surface area of the plate before your spores germinate and effectively smother them. This will also occur at the bottom of the grain jar or cake if you use enough spore solution, but at the top, the excess water drains down or is absorbed, giving a much better chance for your mycelium to get rolling. You may have bacteria in the grain core, but it will likely be surrounded by mycelium, so when you put it on your plate, living mycelium gets the first chance to jump onto the agar.
I really don't suggest tissue to grain, almost impossible to tell if you're gonna waste a ton of time with it.
Okay fair enough well I was planning on injecting atleast half of them my live lions mane mycelium then spawn it to saw dust or whatever. Hsvent looked a ton into the tek just know I can start them on wbs. Was really hoping I could do something fun with the other 5 maybe put spore solution from the diffrent strains in diff ones. Wanna get this fji and Ecuador alive and well.
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