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RowZeZe
Time Watcher


Registered: 12/18/03
Posts: 240
Loc: Space
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Any idea what went wrong?
#26702184 - 05/28/20 04:19 PM (3 years, 7 months ago) |
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This is the first time I’ve come across this problem in 10+ years of growing on/off... All the tubs look like this one. I’ve tried everything from leaving pollyfil out, taping you the holes, dunking the tub. Nothing worked and they all look like this tub. They also smell like very healthy mycelium.
These tubs are over 4 weeks old now, the temp was between 68-75, have a fan on low in the corner. Strain is ‘Cambodia’ from mushrooms.com
Just curious to what it could be because I’ve never had this in like I said 10 years or so....
https://files.shroomery.org/files/20-22/070386546-6B4A90A3-9580-4E81-BE63-88C2237BD882.jpg]
https://files.shroomery.org/files/20-22/070386713-26BB6CA1-3F20-49B0-B165-77E9E4ADB8DB.jpg]
-------------------- Whats done is done and I cannot complain anymore
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RowZeZe
Time Watcher


Registered: 12/18/03
Posts: 240
Loc: Space
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Re: Any idea what went wrong? [Re: RowZeZe]
#26713933 - 06/02/20 12:17 PM (3 years, 7 months ago) |
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?
-------------------- Whats done is done and I cannot complain anymore
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Re: Any idea what went wrong? [Re: RowZeZe]
#26714031 - 06/02/20 01:01 PM (3 years, 7 months ago) |
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It looks like the pins started growing mycelium, but what really happened is that white mycelium is a different species of white mold or a mushroom parasite like Hypomyces sp.
You likely didn't do anything wrong, just unlucky, certain mushroom parasites are seasonal, tending to emerge more in spring and fall, perhaps you just have bad timing.
-------------------- ¯\_(ツ)_/¯
Edited by Tmethyl (06/02/20 01:04 PM)
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: Any idea what went wrong? [Re: Tmethyl]
#26714047 - 06/02/20 01:06 PM (3 years, 7 months ago) |
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What about the likely hood of the spores being compromised in the syringe?
Op did you put them to agar or straight to grain?
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Re: Any idea what went wrong? [Re: Sockadin]
#26714081 - 06/02/20 01:17 PM (3 years, 7 months ago) |
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Quote:
Sockadin said: What about the likely hood of the spores being compromised in the syringe?
I don't believe they would have made it this far if the syringe was contaminated, it would have showed up much sooner, these pins were a few days from maturity.
-------------------- ¯\_(ツ)_/¯
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RowZeZe
Time Watcher


Registered: 12/18/03
Posts: 240
Loc: Space
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Re: Any idea what went wrong? [Re: Sockadin]
#26714676 - 06/02/20 05:05 PM (3 years, 7 months ago) |
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Syringe to jar inoculation
-------------------- Whats done is done and I cannot complain anymore
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: Any idea what went wrong? [Re: RowZeZe]
#26714677 - 06/02/20 05:07 PM (3 years, 7 months ago) |
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Test the syringe on agar.
So do you think it was dirty spawn Tmethyl?
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Re: Any idea what went wrong? [Re: Sockadin]
#26714734 - 06/02/20 05:38 PM (3 years, 7 months ago) |
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I think something moved in on it soon after he spawned, or during the spawn. If it was in spores it would have showed up during the first spawn colonization, it would have stalled out in the jars, and this stuff would have filled in the empty spaces. It never would have made it to full size pins.
It's not a normal or common contamination that's for sure.
-------------------- ¯\_(ツ)_/¯
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: Any idea what went wrong? [Re: Tmethyl]
#26715162 - 06/02/20 09:05 PM (3 years, 7 months ago) |
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Yeah I have seen it before a few times. It just makes me wonder if it is environmental or in the spawn. Thanks.
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