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SoupyGeorge
Hazy Daze since First Grade



Registered: 05/25/20
Posts: 216
Loc: Southern US of 'Merica
Last seen: 1 year, 6 months
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Quote:
Hurrakrayne said: I probably won't clone at the moment. I'm gonna use these plates to to a2g transfers.
I have 5 other small plates I did transfers with healthy mycellium and they look good so I'm using those as well. My grains just got done and I used 2 layers of micropore tape. Hope that's enough. How long should I wait before doing transfers. Pc just reduced to zero pressure?
If you can't wait till morning I'd at least wait until they are comfortable to hold in your hand. You don't want that mycelium hitting the high temperatures that are present right out of the pressure cooker.
I've seen plenty of post saying to pieces of micropore tape is enough, I'm going to poly fill lids myself.
-------------------- Mellow heads will prevail. You look like everyone else because the T.V. tells you to. I look like everyone else so that i don't stand out to the man. We are not the same.
Edited by SoupyGeorge (06/25/20 02:18 PM)
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Hurrakrayne


Registered: 06/26/17
Posts: 217
Last seen: 2 years, 4 months
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Re: My Second Agar Grow [Re: redhandmat] 1
#26782255 - 06/25/20 02:24 PM (3 years, 6 months ago) |
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I wasn't going to put agar into hot grains. I was asking because I wasn't sure if I had to wait 24 hours or if room temp was good. I'm new to agar.
I wasn't gonna drop the mushroom in no. I was gonna cut wedges from the plates and use them that way.
Also I could out another layer or 2 of tape I'm top but that wouldn't be sterile. Unless I used a qtip and swabbed alcohol on top of it after I out the new layers on.
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Hurrakrayne


Registered: 06/26/17
Posts: 217
Last seen: 2 years, 4 months
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Just seen your reply. I've also seen that 2 layers is enough so I'm not too worried about that. Every post I've read has said 2 layers. I have never seen anywhere say 4.
I just grabbed them with nare hands and loosens up the grain so shouldn't be too much longer.
I don't really wanna wait til morning because the pins on the agar already I wanna get he agar to grain as soon as I can.
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SoupyGeorge
Hazy Daze since First Grade



Registered: 05/25/20
Posts: 216
Loc: Southern US of 'Merica
Last seen: 1 year, 6 months
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Im excited for you, cant wait to see the progress.
-------------------- Mellow heads will prevail. You look like everyone else because the T.V. tells you to. I look like everyone else so that i don't stand out to the man. We are not the same.
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Hurrakrayne


Registered: 06/26/17
Posts: 217
Last seen: 2 years, 4 months
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Just did 3 jars but my setup isn't the best and I have to bend a bit and my back is sore lol.
Hope I'm being quick and sterile enough. Gonna do 3 more in a few.
I used 1 plate for 2 jars. Should that be good? Also when I first did an agar transfer I made them in sauce cups so they're not that big. So I dropped one while cuo with good growth into one gonna see how that goes.
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Hurrakrayne


Registered: 06/26/17
Posts: 217
Last seen: 2 years, 4 months
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Also did a tic tac tiger drop on a few of them as well to add more noc points and cut down on the amount of time I have everything exposed.
Seeing as my plates had pins I think my technique is good enough and everything is sterile enough so I shouldn't have too much to worry about.
I used extra alcohol and wiped down constantly.
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Hurrakrayne


Registered: 06/26/17
Posts: 217
Last seen: 2 years, 4 months
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I feel sad having to toss these but I don't have anymore jars.. 🙁 I took all the best mycellium from each of them though.
Wish me luck.
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Kimble
Idiot


Registered: 03/08/18
Posts: 508
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Quote:
Hurrakrayne said:

I feel sad having to toss these but I don't have anymore jars.. 🙁 I took all the best mycellium from each of them though.
Wish me luck.
Those are the chunkiest agar plate pins I've ever seen. To the OP, this all gets easier with practice. My first agar pours were awful and my first transfers all contaminated out. Heck sometimes i msss up a batch now and i started doing this in 08. I'd definitely recommend wrapping your plates. Either saran wrap/clingfilm or parafilm.
You got this. 😎
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Hurrakrayne


Registered: 06/26/17
Posts: 217
Last seen: 2 years, 4 months
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Re: My Second Agar Grow [Re: Kimble]
#26783081 - 06/25/20 07:43 PM (3 years, 6 months ago) |
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Thanks. This is my first pour agar. My first time was with pasty plates and I didn't like it. I've already had more success this way. Definitely gonna wrap from now on. Just hoping the strong myc stays strong through grain colonization.
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SoupyGeorge
Hazy Daze since First Grade



Registered: 05/25/20
Posts: 216
Loc: Southern US of 'Merica
Last seen: 1 year, 6 months
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Quote:
Hurrakrayne said: I used 1 plate for 2 jars. Should that be good? Also when I first did an agar transfer I made them in sauce cups so they're not that big. So I dropped one while cuo with good growth into one gonna see how that goes.
Quote:
Hurrakrayne said: Also did a tic tac tiger drop on a few of them as well to add more noc points and cut down on the amount of time I have everything exposed.
The outcome of the different inoculation techniques; half patties, whole patties, and tic tac tiger intrigue me. Please let me know if there is a noticeable difference in time to colonize as I will be at this stage with agar before long and would like to know what worked best for you.
-------------------- Mellow heads will prevail. You look like everyone else because the T.V. tells you to. I look like everyone else so that i don't stand out to the man. We are not the same.
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Hurrakrayne


Registered: 06/26/17
Posts: 217
Last seen: 2 years, 4 months
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Will do. I did a few different techniques to see which works best. I just picked my agar pins and they're pretty much full blown shrooms. Should done it last night thought as they have already dropped there spores. But they smell like nice fresh mushrooms.
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Hurrakrayne


Registered: 06/26/17
Posts: 217
Last seen: 2 years, 4 months
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Got some minor growth starting!
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AtmozFear
just a shade of myself


Registered: 01/25/19
Posts: 1,032
Last seen: 21 hours, 41 minutes
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i guess wait to see if it likes your grain prep
btw two layers of micropore tape is fine.
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Hurrakrayne


Registered: 06/26/17
Posts: 217
Last seen: 2 years, 4 months
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Re: My Second Agar Grow [Re: AtmozFear]
#26793698 - 06/30/20 04:35 AM (3 years, 6 months ago) |
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Couldn't get a clear picture of the grains but about half of my jars are showing alot of growth and I put one of the pins on agar and it's showing nice mycellium.
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Hurrakrayne


Registered: 06/26/17
Posts: 217
Last seen: 2 years, 4 months
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Looking good!! When should I start to shake these up?
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Hurrakrayne


Registered: 06/26/17
Posts: 217
Last seen: 2 years, 4 months
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So I just shook them all except the one that looks almost done. The mycellium looks beautiful hopefully I didn't just ruin them.
My first time doing agar I shook them and they all died on me. But that was due to contamination and I wasn't being as sterile so I think I'm good. Will post more photos when I see some recovery.
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SoupyGeorge
Hazy Daze since First Grade



Registered: 05/25/20
Posts: 216
Loc: Southern US of 'Merica
Last seen: 1 year, 6 months
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Awesome, keep us posted. I love watching other grows from beginning to end.
-------------------- Mellow heads will prevail. You look like everyone else because the T.V. tells you to. I look like everyone else so that i don't stand out to the man. We are not the same.
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Hurrakrayne


Registered: 06/26/17
Posts: 217
Last seen: 2 years, 4 months
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Thanks. Will do! I just went on a hunt for coir locally and noone has it or knew what it was. Small town problems. Lol. So I just ordered some and it should be here by Monday. Hopefully I can put everything to fruiting conditions that day.
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SoupyGeorge
Hazy Daze since First Grade



Registered: 05/25/20
Posts: 216
Loc: Southern US of 'Merica
Last seen: 1 year, 6 months
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Quote:
Hurrakrayne said: Thanks. Will do! I just went on a hunt for coir locally and noone has it or knew what it was. Small town problems. Lol. So I just ordered some and it should be here by Monday. Hopefully I can put everything to fruiting conditions that day.
Did you try a pet store, They would be the only brick and mortar place to carry it. I get mine from a shabby corner pet store next door to the washateria.
-------------------- Mellow heads will prevail. You look like everyone else because the T.V. tells you to. I look like everyone else so that i don't stand out to the man. We are not the same.
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Hurrakrayne


Registered: 06/26/17
Posts: 217
Last seen: 2 years, 4 months
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There isn't any more petstores in my area. They all got shut down. I ordered it from Petco.
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