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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: redhandmat]
    #26781250 - 06/25/20 06:19 AM (3 years, 6 months ago)

No they're not wrapped.
To clone I would just cut it at the base and drop it on new agar plate correct? Or is this where in supposed to cut it open and take inner tissue?


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: AtmozFear]
    #26781255 - 06/25/20 06:21 AM (3 years, 6 months ago)

Ok I'll keep that in mind. New to agar so I've never even seen this much success.
Definitely gonna do g2g on maybe half my jars to see the difference.


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OfflineAtmozFear
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Re: My Second Agar Grow [Re: redhandmat]
    #26781256 - 06/25/20 06:24 AM (3 years, 6 months ago)

the fact that the agar is so healthy it's producing fruit shows there is NOT contamination imo... I don't think you should clone those just make new transfers like you were asking.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: AtmozFear]
    #26781259 - 06/25/20 06:28 AM (3 years, 6 months ago)

Ok I have 5 plates I made when I did first set of transfers to agar but I left them in my SAB. Are they still good to use or should I make more?

Also for my grain jar lids. Do they need the holes punched on top with polyfil or micropore tape? Or can I just loosen them slightly?


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: AtmozFear]
    #26781264 - 06/25/20 06:32 AM (3 years, 6 months ago)

I also wanna use them to transfer directly to grain seeing that they're healthy and no signs of contam.


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OfflineAtmozFear
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26781269 - 06/25/20 06:35 AM (3 years, 6 months ago)

as long as there is no visible contamination, they are usable.  Never loosen the lid... I always punch two holes and put two layers of micropore tape on each hole.  You're doing great. I have yet to see fruits from my agar, wish I did!


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Edited by AtmozFear (06/25/20 06:36 AM)


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: AtmozFear]
    #26781272 - 06/25/20 06:37 AM (3 years, 6 months ago)

That's what I figured. I just thought I read somewhere it's been done with just loosening.
Do holes have to be certain size? I only have small drill bits. No bigger than maybe a 1/4 inch.
Sorry for all the noob questions.


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OfflineAtmozFear
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26781278 - 06/25/20 06:41 AM (3 years, 6 months ago)

that's perfect size- I just run a 1/4" nail through the lid and tape it up.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: AtmozFear]
    #26781283 - 06/25/20 06:44 AM (3 years, 6 months ago)

Cool cool. I appreciate your quick feedback.
Will post when I have more updates.


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OfflineAtmozFear
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26781295 - 06/25/20 06:54 AM (3 years, 6 months ago)

no problem... i actually just started pouring agar myself.  Up until now, I had been doing no pour.  lol someone on here said no pour is for pussies... he was right!  so you're def challenging yourself and learning fast.  Parafilm application is what gets me.


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Edited by AtmozFear (06/25/20 06:57 AM)


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: AtmozFear]
    #26781316 - 06/25/20 07:10 AM (3 years, 6 months ago)

Yeah I tried no pour my first time and did not like it. This is more fun. Lol
Also I just noticed I got cloth tape . Is that gonna make a difference? It's waterproof and was with all the surgical and micropore tape. Or should I go get a different kind?


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OfflineSoupyGeorge
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26781333 - 06/25/20 07:20 AM (3 years, 6 months ago)

Quote:

Hurrakrayne said:
Yeah I tried no pour my first time and did not like it. This is more fun. Lol
Also I just noticed I got cloth tape . Is that gonna make a difference? It's waterproof and was with all the surgical and micropore tape. Or should I go get a different kind?




So my input here is only from one experience. But I inoculated 9 jars with a few different methods. (I also bought the cloth tape) So due to not knowing whether it would allow gas exchange I used multiple methods. The two jars that I put the cloth tape on were light years behind the other jars in colonization. So my educated guess is do not use that cloth tape.


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Mellow heads will prevail.

You look like everyone else because the T.V. tells you to.
I look like everyone else so that i don't stand out to the man.
We are not the same.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: SoupyGeorge]
    #26781342 - 06/25/20 07:23 AM (3 years, 6 months ago)

Dammit. Lol.
Ok soon as the grains are done boiling I'll go get different tape.


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OfflineAtmozFear
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26781415 - 06/25/20 07:53 AM (3 years, 6 months ago)

i agree; and the same company that makes the medical grade micropore tape also makes the proper face masks some people are wearing... if that means anything :shrug:


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Edited by AtmozFear (06/25/20 07:54 AM)


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: AtmozFear]
    #26781425 - 06/25/20 07:56 AM (3 years, 6 months ago)

It does lol. Heading to the store now grains just got done boiling.


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OfflineAtmozFear
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26781468 - 06/25/20 08:10 AM (3 years, 6 months ago)

:thumbup::thumbup:


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: AtmozFear]
    #26781977 - 06/25/20 12:20 PM (3 years, 6 months ago)

How long should I let my jars cool before doing transfers?


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Offlineredhandmat
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Re: My Second Agar Grow [Re: AtmozFear]
    #26782008 - 06/25/20 12:33 PM (3 years, 6 months ago)

Quote:

AtmozFear said:
the fact that the agar is so healthy it's producing fruit shows there is NOT contamination imo... I don't think you should clone those just make new transfers like you were asking.




This is not always true though. It happens often that plates pin due to stress. However, as far as I understand those plates usually show obvious stress. These plates probably pinned because myc had taken all nutrients and FAE was introduced as he didnt wrap the petris. Fortunately OP seems to have left the plates inside the SAB. They probably would have contaminated otherwise.


Moving on to the cloning. I wouldnt slice open the mushrooms, instead I would tear it in two inside the SAB, then take a flamed scalpel and take a tiny bit from the inside and put it on agar. If you slice it open you could introduce contams from the outside (but thats not probable because you've had your plates inside the SAB).

As for the grain jars, I usually just make my 4-5 holes with a nail and hammer. On shroomery most advanced cultivators suggest using polyfill as a filter, or a proper filter disk. I use micropore, but then you have to put at least 4 layers or more.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: redhandmat]
    #26782078 - 06/25/20 01:00 PM (3 years, 6 months ago)

I probably won't clone at the moment. I'm gonna use these plates to to a2g transfers.

I have 5 other small plates I did transfers with healthy mycellium and they look good so I'm using those as well.
My grains just got done and I used 2 layers of micropore tape. Hope that's enough.
How long should I wait before doing transfers.
Pc just reduced to zero pressure?


Edited by Hurrakrayne (06/25/20 01:01 PM)


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Offlineredhandmat
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26782238 - 06/25/20 02:15 PM (3 years, 6 months ago)

Quote:

Hurrakrayne said:
I probably won't clone at the moment. I'm gonna use these plates to to a2g transfers.

I have 5 other small plates I did transfers with healthy mycellium and they look good so I'm using those as well.
My grains just got done and I used 2 layers of micropore tape. Hope that's enough.
How long should I wait before doing transfers.
Pc just reduced to zero pressure?




So you will br droping the mushroom with the agar inside grain jars? Why not just use tweezers to remove the shrooms? I dont think 2 layers is enough for grain jars, but you could always experiment and see what happens. What do you mean how long? till you put agar to grains? once you pc your jars for 90+ min at 15 psi, let jars cool down to room temperature and then drop your agar inside. N point in ruining your myc by putting it in very hot grains.


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