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Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: My Second Agar Grow [Re: Hurrakrayne]
    #26781220 - 06/25/20 05:52 AM (3 years, 7 months ago)

Quote:

Hurrakrayne said:
3 weeks. 6/24



So I haven't checked my plates in a few days and it's been really hot and muggy and I check today and I have these!
Those are the original plates I did transfers off and thought they were no good.  I wasn't even gonna use them. Can I still transfer these to grain even though they have pins?

Also my new plates that I transferred to have nice rhizomorph growth I just couldn't get a good enough picture.




Are your plate wrapped? If nto it could explain the quick invitro pinning, as they are getting FAE. Anyways if I were you, I would clone those pins on new plates. Make sure to wrap your plates so you dont get contams in.


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Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: My Second Agar Grow [Re: AtmozFear]
    #26782008 - 06/25/20 12:33 PM (3 years, 6 months ago)

Quote:

AtmozFear said:
the fact that the agar is so healthy it's producing fruit shows there is NOT contamination imo... I don't think you should clone those just make new transfers like you were asking.




This is not always true though. It happens often that plates pin due to stress. However, as far as I understand those plates usually show obvious stress. These plates probably pinned because myc had taken all nutrients and FAE was introduced as he didnt wrap the petris. Fortunately OP seems to have left the plates inside the SAB. They probably would have contaminated otherwise.


Moving on to the cloning. I wouldnt slice open the mushrooms, instead I would tear it in two inside the SAB, then take a flamed scalpel and take a tiny bit from the inside and put it on agar. If you slice it open you could introduce contams from the outside (but thats not probable because you've had your plates inside the SAB).

As for the grain jars, I usually just make my 4-5 holes with a nail and hammer. On shroomery most advanced cultivators suggest using polyfill as a filter, or a proper filter disk. I use micropore, but then you have to put at least 4 layers or more.


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Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: My Second Agar Grow [Re: Hurrakrayne]
    #26782238 - 06/25/20 02:15 PM (3 years, 6 months ago)

Quote:

Hurrakrayne said:
I probably won't clone at the moment. I'm gonna use these plates to to a2g transfers.

I have 5 other small plates I did transfers with healthy mycellium and they look good so I'm using those as well.
My grains just got done and I used 2 layers of micropore tape. Hope that's enough.
How long should I wait before doing transfers.
Pc just reduced to zero pressure?




So you will br droping the mushroom with the agar inside grain jars? Why not just use tweezers to remove the shrooms? I dont think 2 layers is enough for grain jars, but you could always experiment and see what happens. What do you mean how long? till you put agar to grains? once you pc your jars for 90+ min at 15 psi, let jars cool down to room temperature and then drop your agar inside. N point in ruining your myc by putting it in very hot grains.


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