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OfflineHurrakrayne
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Registered: 06/26/17
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My Second Agar Grow * 1
    #26701575 - 05/28/20 11:12 AM (3 years, 7 months ago)

Hows it going everyone??


So i started my second try at growing with agar. My first try was with Pasty's mini rounds and i didnt have great success. Im using bods tek this time around.

So this time i went with  no pour and still used BRF. The agar this time looked 10 times better than last time so thats a plus. I have 20 plates poured as well. I also sanitized alot more and tried to be more thorough.

Gonna post photos when i start to get mycelium on the plates.

Peace!


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: jay.ach]
    #26708554 - 05/31/20 06:36 AM (3 years, 7 months ago)

Bad technique. I don't think I was sanitizing as much as I should or I did the grains wrong. I had beautiful agar growth and transferred to grain and started and it started colonizing then everything turned black and greenish.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26708582 - 05/31/20 06:59 AM (3 years, 7 months ago)

Day 3..
This is what I have so far. A little condensation but I don't think that should be too much of an issue.
The 2 on the outside of the SAB had the most condensation so I figured I would knock them up in open air and see what happens and they also have some mycellium starting. Everything is looking good so far. Will post more when pics when I can.



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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26713752 - 06/02/20 11:02 AM (3 years, 7 months ago)

Day 5..
Got more growth. The temps dropped in my area a bit. Mid 50s overnight. Hoping that won't be too much of an issue. The loner is the one I nocced in open air. Seems to be good so far.




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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26721053 - 06/05/20 06:28 AM (3 years, 7 months ago)

Day 7...
Looking good. One plate I nocced in open air contammed but the other one is thriving and no other contams yet.



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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: Trippypete]
    #26733626 - 06/10/20 06:52 AM (3 years, 7 months ago)

Day 12...

Mycellium seems to be taking hold and getting thicker by the day.



Edited by Hurrakrayne (06/10/20 06:53 AM)


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26736040 - 06/11/20 05:41 AM (3 years, 7 months ago)

Gonna do some transfers today. I don't have anymore empty plates so I'm gonna use the cups that pizza places out hot sauce in. Lol. Idk the proper name for them. 2 more plate have contams so I wanna clean them up a bit.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: jay.ach]
    #26736157 - 06/11/20 07:32 AM (3 years, 7 months ago)

I've done everything in the SAB. I nocced 2 plates in open air for the hell of it and experimentation purposes and they both contammed. The second one I can probably get a decent transfer from since only half is contammed but I'm not gonna chance it.
One of them got all cobweb moldy and the third has a small black spot but amazing mycellium growth the rest of the plate.

This will be my first time doing a agar transfer so hoping all goes well. Definitely gonna invest in a flow good for the future.
My biggest concern so far is that I don't have much rhizomorphic growth. Mostly the fluffy white stuff. About 5 plates have rhizomorph starting towards the edges. Another reason I wanna transfer today and clean them up.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26744011 - 06/14/20 12:11 PM (3 years, 7 months ago)

So the transfers went good. Starting to see the mycellium grab hold of the new agar. I've never felt this excited lol.
Will post pictures when possible.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: Wall.E]
    #26779500 - 06/24/20 02:08 PM (3 years, 7 months ago)

3 weeks. 6/24



So I haven't checked my plates in a few days and it's been really hot and muggy and I check today and I have these!
Those are the original plates I did transfers off and thought they were no good.  I wasn't even gonna use them. Can I still transfer these to grain even though they have pins?

Also my new plates that I transferred to have nice rhizomorph growth I just couldn't get a good enough picture.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: EntheoGod]
    #26781195 - 06/25/20 05:31 AM (3 years, 7 months ago)

So those pins opened up over night and dropped spores..

Can I still use these plates to transfer to grain?
I'm starting to run my cycle of grains now.


Edited by Hurrakrayne (06/25/20 05:32 AM)


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: AtmozFear]
    #26781213 - 06/25/20 05:47 AM (3 years, 7 months ago)



One is a side view the other is a top view. There's multiple plates like that with pins and dropped spores. I'm gonna transfer directly from these plates plus some other transfer plates I have going.

Is it necessary to do g2g? Or does that just increase the amount?


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Re: My Second Agar Grow [Re: redhandmat]
    #26781250 - 06/25/20 06:19 AM (3 years, 6 months ago)

No they're not wrapped.
To clone I would just cut it at the base and drop it on new agar plate correct? Or is this where in supposed to cut it open and take inner tissue?


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: AtmozFear]
    #26781255 - 06/25/20 06:21 AM (3 years, 6 months ago)

Ok I'll keep that in mind. New to agar so I've never even seen this much success.
Definitely gonna do g2g on maybe half my jars to see the difference.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: AtmozFear]
    #26781259 - 06/25/20 06:28 AM (3 years, 6 months ago)

Ok I have 5 plates I made when I did first set of transfers to agar but I left them in my SAB. Are they still good to use or should I make more?

Also for my grain jar lids. Do they need the holes punched on top with polyfil or micropore tape? Or can I just loosen them slightly?


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Re: My Second Agar Grow [Re: AtmozFear]
    #26781264 - 06/25/20 06:32 AM (3 years, 6 months ago)

I also wanna use them to transfer directly to grain seeing that they're healthy and no signs of contam.


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Re: My Second Agar Grow [Re: AtmozFear]
    #26781272 - 06/25/20 06:37 AM (3 years, 6 months ago)

That's what I figured. I just thought I read somewhere it's been done with just loosening.
Do holes have to be certain size? I only have small drill bits. No bigger than maybe a 1/4 inch.
Sorry for all the noob questions.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: AtmozFear]
    #26781283 - 06/25/20 06:44 AM (3 years, 6 months ago)

Cool cool. I appreciate your quick feedback.
Will post when I have more updates.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: AtmozFear]
    #26781316 - 06/25/20 07:10 AM (3 years, 6 months ago)

Yeah I tried no pour my first time and did not like it. This is more fun. Lol
Also I just noticed I got cloth tape . Is that gonna make a difference? It's waterproof and was with all the surgical and micropore tape. Or should I go get a different kind?


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: SoupyGeorge]
    #26781342 - 06/25/20 07:23 AM (3 years, 6 months ago)

Dammit. Lol.
Ok soon as the grains are done boiling I'll go get different tape.


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