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Offlineadahl
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Morel to Agar
    #26697658 - 05/26/20 06:10 PM (3 years, 8 months ago)

I found a patch of morel mushrooms and saved one for some tissue samples that I could transfer to agar. what do you think of this growth?


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InvisibleApples in Mono
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Registered: 09/21/17
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Re: Morel to Agar [Re: adahl]
    #26697791 - 05/26/20 07:18 PM (3 years, 8 months ago)

I think it's pretty awesome looking. But also not like any picture of a morel culture I've ever seen


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InvisibleCaps McGee
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Re: Morel to Agar [Re: Apples in Mono]
    #26697916 - 05/26/20 08:08 PM (3 years, 8 months ago)

Its bacterial and trying to escape... I dont think its salvageable... morel is one of those things you spawn and either get lucky or not... I wouldnt invest much time in it personally: just return to the patch each year as it will return... always leave a few to decompose in the area


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OfflineMad Hatter
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Re: Morel to Agar [Re: adahl]
    #26698014 - 05/26/20 08:59 PM (3 years, 8 months ago)

Thats not morel myceliem. Morels will produce metabolites like crazy. So much that it will change the color of mycelium from white to yellow to brown. Its also fluffier (in my experience) on agar and will produce scelrotia.

From left to right. Trametes versicolor (for comparison of color), morchella importuna, morchella angusticeps, morchella ruffrobrunes, and morchella esculentoides. Sorry if spelling is off. I have a morchella punctipes im cleaning up now not enough growth for pic.



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InvisibleApples in Mono
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Re: Morel to Agar [Re: Mad Hatter]
    #26698020 - 05/26/20 09:02 PM (3 years, 8 months ago)

I've heard it's usually easier to get a clean morel culture with spores from a dried fruit instead of a tissue sample


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OfflineMad Hatter
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Re: Morel to Agar [Re: Apples in Mono]
    #26698037 - 05/26/20 09:21 PM (3 years, 8 months ago)

If you can clone a morel you can probably clone literally any mushroom. It is very difficult. The importuna and ruffrobrunes I got online but the others I've cloned.

If you want to clone a morel you need a dish that has no condensation in it, dip morel in a iodine solution, and hope for a thick walled stipe since they are hollow. 

I literally riped up about 10 morels just to find a good thick stipe wall. Bacteria is you biggest enemy with morels so adding gentamycin to youre agar would be beneficial. My gentamycin arrived the day after i got clean growth......

The patches i found were worth cloning imho. My esculentoides is from a morel that was about 8 inches tall.

Havent tried spores yet so cant comment on that


Edited by Mad Hatter (05/26/20 09:24 PM)


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OfflineBigGameHunter
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Re: Morel to Agar [Re: Mad Hatter]
    #26698788 - 05/27/20 07:23 AM (3 years, 8 months ago)

I too find cloning morels very difficult. For an actual clone attempt, I usually end up shaving the inside of the stipe or fruiting body with a scalpel or dental pick to avoid spores and putting it on agar. Bacteria contamination still usually happens but if the morel mycelium starts to grow it usually grows very fast and goes aerial later in its growth stage. If it grows to area where there's no visible contamination I take a wedge & transfer or if there is visible contamination I sometimes have success just transferring some of the aerial strands.


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Offlineadahl
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Re: Morel to Agar [Re: BigGameHunter]
    #26699224 - 05/27/20 10:56 AM (3 years, 8 months ago)

Are the strands I have growing on this dish mycelium?


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OfflineBigGameHunter
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Re: Morel to Agar [Re: adahl]
    #26699259 - 05/27/20 11:27 AM (3 years, 8 months ago)

In my experience that doesn't look like morel mycelium. IME it starts out as fine & wispy almost invisible unless the light is glaring at the proper angle. Then it starts to become aerial & fluffy but not as dense as some mycelium when it gets fluffy. By this time the mycelium has already covered the entire surface of the plate. Then you may see white specs (sclerlotia) form on the surface of the plate. At the same time the aerial/fluffy mycelium starts to change color toward a rust/brown color. Then later the the specs that were mentioned earlier start turning rust/brown. The characteristics that I witness may also be due to the type/amounts of nutrients in my agar mix. I haven't experimented much in that regard. I'll try to post an image of a plate I have in the next day or so.


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Offlineadahl
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Re: Morel to Agar [Re: BigGameHunter]
    #26699401 - 05/27/20 01:02 PM (3 years, 8 months ago)

Thanks for the info! Have you tried transferring that to a jar yet? Or tried fruiting it even?


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OfflineMad Hatter
10/6
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Re: Morel to Agar [Re: BigGameHunter]
    #26699616 - 05/27/20 02:16 PM (3 years, 8 months ago)

Sounds on par with my experience as well. The nearly invisible growth and aerial strands i have both experienced aswell.


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OfflineMad Hatter
10/6
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Registered: 12/20/18
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Re: Morel to Agar [Re: adahl]
    #26699659 - 05/27/20 02:31 PM (3 years, 8 months ago)

Ive made sawdust/compost spawn with morels they do fine on grain but can look very odd can almost look contaminated but isnt. Once added to sawdust/compost the colonization is very different than other more typical edibles. You wont get the pure white growth youre used to they tend to produce scelrotia very heavily and will make a very hard outside surface to the bag. Like rock hard where they form.

I spawned some importuna and rufobrunnes to the hugelkulture i made this spring. These are 2 good garden morels as they dont need a mycorrhizal partner like most morels. Ive tried fruiting them indoors once with no success. If you want to try them indoors these 2 are your best bet but still slim chances due to environmental needs. Getting a single morel even a single pin to form would be considered a good reason for celebrating.

Any other species of morel you are going to want to try to inoculate tree roots.


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