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OfflineLunatikPenguin
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Mycelium or mold/something else?
    #26697719 - 05/26/20 06:46 PM (3 years, 7 months ago)

I had less than stellar results with my first set of agar plates, lots of condensation. I inoculated several with spore syringe using a drop (well, tried to only do a drop...) and smearing with a flamed loop. I have a couple of plates that have spots like this, is it Myc or contam? The pic is taken from below, as the lid has too much condensation on it.



Next time I inoculate from syringe I think I'll use a sterile shot glass with a small bit of the syringe contents in it and dip my flamed loop in there after allowing it to cool (not sure exactly how I'd do that yet, just stick it in the edge of a plate then dip in the shot glass?).

Thanks!


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InvisibleCaps McGee
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Re: Mycelium or mold/something else? [Re: LunatikPenguin]
    #26697936 - 05/26/20 08:20 PM (3 years, 7 months ago)

Looks like mycelium but needs some work: it looks like it might have a halo, which could indicate bacteria... I'd give it a few days to see if you get any off shoots you can take a timely transfer of and escape

Best to flame the end of the syringe and drop a single drop directly to agar, AFTER running some solution out to cool the needle... squeeze the plunger at the opening in the back of the syringe to control your pressure/amount administered... conversely, can be applied to a flamed loop directly with the syringe and then streaked... the fewer tools and surfaces between syringe and agar the better... fewer vectors for contamination


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OfflineLunatikPenguin
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Re: Mycelium or mold/something else? [Re: Caps McGee]
    #26699544 - 05/27/20 01:47 PM (3 years, 7 months ago)

Ok thanks, I'll try to make a transfer. Hopefully I'll get some offshoots and can isolate the Myc.

As for technique, I failed to mention I did cool the needle by squirting a little out. Good call on the method to try to slow down the amount of liquid being dispersed. I'll maybe try the syringe to loop method. Makes sense about avoiding the shot glass and having another surface to worry about. Thanks!


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