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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Spawn bag contamination??
#26695744 - 05/25/20 08:51 PM (3 years, 8 months ago) |
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So I have a couple of bags that have some weird occurrences I’ve never experienced, they are almost 100% but than have these weird patches is this bacterial contamination? Bags were prepared using the no rinse soak or boil just weights out millet add weighted water 1125 grams grain to 525 grams water. If I find my grains water content through the oven method how do I determine how much water to add?
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Any advice??
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 6 months, 14 days
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How long have they been colonizing? Depending on how long they've been going for, uncolonized sections can often times indicate a bacterial contamination. There are some parts of the myc that do look a little thick and indicative of bacterial contam. However, I'm on my phone and can't get a great view at the moment. I'll check back when I get home from work, but hopefully someone else that can view the pictures better will have chimed in by then 
Good news is even if they are bacterial, if they get to 100% colonization you can still fruit it. Often times, my bacterial tubs spit out some gnarly looking mutants.
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Re: Spawn bag contamination?? [Re: Gan]
#26696674 - 05/26/20 10:17 AM (3 years, 8 months ago) |
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K. They been going for about 2 weeks but I shook them twice could have allowed it to get a foothold. Is there anything in my prep I can change to prevent this?
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 6 months, 14 days
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I can't say exactly how long they SHOULD take if healthy, as I've never experimented with bags, only jars. But I'm sure someone else can.
As for prep, your grain does look on the wet side (looks like WBS, right?). Assuming you are pc'ing your grain for the appropriate amount of time and you are letting your PC properly vent and it's staying up at the correct pressure the entire time, there's nothing more in your prep that you can necessarily do to prevent contamination. However, I will add that having grain on the wet side does NOT make spawn bacterial, but it can create a more suitable environment for bacteria to take hold and expand. So just play around with your hydration of your grains. You'll find the sweet spot. Make sure you take notes on each prep until you figure it out.
Mostly, just work on improving your sterile technique while working with your cultures and spawn. Make sure you are inoculating your spawn with a clean agar culture/LC. How did you inoculate your bag? Agar wedge or straight from a spore syringe?
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micelio
Song of Silence


Registered: 04/22/14
Posts: 1,547
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Re: Spawn bag contamination?? [Re: Gan]
#26696704 - 05/26/20 10:31 AM (3 years, 8 months ago) |
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Pic.#4.. You can see the grain slimmed to the surface of the bag (bacteria)
All four pics. seemed to show allot of water.
When I do grain I like keeping the grain as dry as possible after hydrating It..
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 6 months, 14 days
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Re: Spawn bag contamination?? [Re: micelio]
#26696723 - 05/26/20 10:39 AM (3 years, 8 months ago) |
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Good to know I can see decently with my phone then, cause yeah the top right in pic #4 is what was suggesting bacterial to me.
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Re: Spawn bag contamination?? [Re: Gan]
#26696745 - 05/26/20 10:47 AM (3 years, 8 months ago) |
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So I used LC that was vetted through agar and grain. Im prepping per this tek https://www.shroomery.org/forums/showflat.php/Number/26584191 but I’m wondering if I could determine my own millet water content by placing 100g in to the oven for hour and determine my own water weight. But I wouldn’t know the amount needed to hydrate the grain anyone with experience in this would be great. Also debating presale but that messes with my water content right?
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Can anyone help with the oven question here??
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