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OfflineZoda
Pilzkopf
I'm a teapot


Registered: 11/17/19
Posts: 39
Loc: Germany Flag
Last seen: 20 days, 15 hours
Re: Cobweb mold in Substrate bag? [Re: LogicaL Chaos]
    #26691800 - 05/24/20 04:56 AM (3 years, 8 months ago)

Hey guys, quick update from my grow:

I did go against you guys decision to dunk the cake, as it just looked to fragile in my opinion and ive read about cakes becoming completely washed out and since I had twice the amount of perlite in this time and chunks were coming out already...I decided to heavy mist (also on the cake itself) for the first two days after harvesting the 2nd flush and just keep misting properly after that.

We can definately conclude , your analysis of the problem being with airflow was spot on! The shotgun box is now producing fruit bodies of the size I usually know :smile:



So as you see only 4 larger shrooms, seems my only problem remaining is to get a clean culture going to spawn proper cakes.

Sadly, the two jars i filled up with my best looking agar dishes have turned out stalling after initial shake after a week (around 20% colonization at that point). and now 10 days after shaking nothing is really coming back...im preparing some more transfers now and probably will also try to take some more fresh samples from the shrooms and also maybe some spore prints?

I just have to take off the head and put in on aluminum foil into the sun, right?

Hope everyone has had a great weekend and we all can start into a good week for us.


--------------------
“I’m growing mushrooms… because I can’t kill them. They just keep multiplying…and it’s like…I’m in service to them.”


Extras: Filter Print Post Top
OfflineZoda
Pilzkopf
I'm a teapot


Registered: 11/17/19
Posts: 39
Loc: Germany Flag
Last seen: 20 days, 15 hours
Re: Cobweb mold in Substrate bag? [Re: Zoda]
    #26694146 - 05/25/20 03:55 AM (3 years, 8 months ago)

I got my first 3 solid spore prints!



So after I have transfered my current petri dishes into jars to see how theyre doing, I will attempt my first petri dish with spores. Very exciting! :smile:


--------------------
“I’m growing mushrooms… because I can’t kill them. They just keep multiplying…and it’s like…I’m in service to them.”


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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,842
Loc: In the Gills
Last seen: 3 hours, 24 minutes
Re: Cobweb mold in Substrate bag? [Re: Zoda]
    #26694941 - 05/25/20 12:48 PM (3 years, 8 months ago)

Making a print is for long term preservation of your variety. It's best
to keep working from cloned flesh and not spores.

If you post some pics of ur dishes and jars I'll try to offer any help
there as well


--------------------

Links For Beginners And Beyond:mspoil:


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OfflineZoda
Pilzkopf
I'm a teapot


Registered: 11/17/19
Posts: 39
Loc: Germany Flag
Last seen: 20 days, 15 hours
Re: Cobweb mold in Substrate bag? [Re: rumfor69]
    #26695080 - 05/25/20 02:03 PM (3 years, 8 months ago)

Quote:

rumfor69 said:
Making a print is for long term preservation of your variety. It's best
to keep working from cloned flesh and not spores.

If you post some pics of ur dishes and jars I'll try to offer any help
there as well




Hm maybe I will give it a try just for practise sakes and hey, maybe itll be a much better culture than what I currently got:



This should be the 7th generation of that single grain that i isolated weeks ago.

They look pretty good imo, but so did the two from 2 weeks ago. Now thinking about it...I had to change the silicone nob at the pressure valve of my cooking pot and I think the two jars only got around 1,5 levels of the 2 (which is 115psi i believe). so maybe the jars werent fully sterilized after all. but i think im quite good on the jar side, as i had phenomenal jars already that fully colonized within 2 weeks. but then something went wrong when introducing them to my substrate.

I guess I will just have to keep trying spawn a bag with this generation and see how different the yields are?

Hope youre having a great start in your week and happy to hear any word of advise :smile:


--------------------
“I’m growing mushrooms… because I can’t kill them. They just keep multiplying…and it’s like…I’m in service to them.”


Extras: Filter Print Post Top
Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,842
Loc: In the Gills
Last seen: 3 hours, 24 minutes
Re: Cobweb mold in Substrate bag? [Re: Zoda]
    #26695397 - 05/25/20 05:18 PM (3 years, 8 months ago)

Damn those dishes look good

It might be that the jars didn't cook right maybe. If you're using a
SAB instead of a flowhood there is also a dice roll chance of contamination
from the air.


--------------------

Links For Beginners And Beyond:mspoil:


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OfflineZoda
Pilzkopf
I'm a teapot


Registered: 11/17/19
Posts: 39
Loc: Germany Flag
Last seen: 20 days, 15 hours
Re: Cobweb mold in Substrate bag? [Re: rumfor69]
    #26696277 - 05/26/20 05:38 AM (3 years, 8 months ago)

Quote:

rumfor69 said:
Damn those dishes look good

It might be that the jars didn't cook right maybe. If you're using a
SAB instead of a flowhood there is also a dice roll chance of contamination
from the air.





i hope those jars that ill make today will look just as good in 2 weeks time :smile:



heres my "flowhood" from before. its basically a room air purifyer but it does have some good suction behind it! I think I might try it again, since Ive always used it as the underground of where to place my jars. But all the videos of flowhoods ive seen placed them BEHIND the jars, so i think my placement mightve actually caused contams.

Right next to it my SAB with equipment i use.


--------------------
“I’m growing mushrooms… because I can’t kill them. They just keep multiplying…and it’s like…I’m in service to them.”


Extras: Filter Print Post Top
OfflineZoda
Pilzkopf
I'm a teapot


Registered: 11/17/19
Posts: 39
Loc: Germany Flag
Last seen: 20 days, 15 hours
Re: Cobweb mold in Substrate bag? [Re: Zoda]
    #26751876 - 06/17/20 02:41 PM (3 years, 7 months ago)

Update: I have now gotten successful fresh samples without contam from the last flush! All the grain jars i made three weeks ago turned out to be stalling.

Which other cutting decisions would you have made? :smile:



--------------------
“I’m growing mushrooms… because I can’t kill them. They just keep multiplying…and it’s like…I’m in service to them.”


Extras: Filter Print Post Top
Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,842
Loc: In the Gills
Last seen: 3 hours, 24 minutes
Re: Cobweb mold in Substrate bag? [Re: Zoda]
    #26752347 - 06/17/20 06:00 PM (3 years, 7 months ago)

Those are the areas I would've cut from so you're doing it right.
My only suggestion would be to try to take smaller cuttings in the ropes.
Like piece the size of a grain. Even the smallest rope filament will work
and it will take less transfers to get to a monoculture. Monocultures aren't

necessarily required to have nice, successful, profitable grows. A clone
from flesh itself is still like 100 genetics instead of billions like
with spores.(numbers aren't exact just a concept) Even a nice monoculture
that looks perfect still has a bunch of different genetics all tightly
braided together in those ropes.

Also maybe implement a labeling system like A1, A2, B1,B2, A1-T1,A1-T2 to
to keep track of which transfer generation you're on


--------------------

Links For Beginners And Beyond:mspoil:


Edited by rumfor69 (06/17/20 06:04 PM)


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