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Offlinezakzwijn
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my first and second monotub, will update during progress - update: ongoing harvest and some microscope pics * 1
    #26694818 - 05/25/20 11:58 AM (3 years, 8 months ago)

Hi fellow shroomers, welcome to my first monotub project with around 2.5-3 Liters of rye spawn in it. The holes are covered with cotton pads. I Was going for polyfills first but the store I was in ran out so I decided to give cotton pads a try. In addition to the four holes you can see the tub as two more holes on each side (also covered with cotton pads) higher so fresh air can flow in and co2 can flow out. I know it's better to build up CO2 first but I decided to start slow so I'm following the sit and forget approach, no misting or fanning as well. I followed this method for the substrate.

Humidity seems to be okay, it's a bit hard to see in this picture but you can see droplets on te sides and there's definitely condensation going on there, also on the mycelium itself. I put it in normal daylight on my desk, no direct sunlight. What do you all think? Shoot :smile:



Edited by zakzwijn (06/18/20 10:11 AM)


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OfflineSenku
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Re: my first monotub, will update during progress [Re: zakzwijn]
    #26694915 - 05/25/20 12:40 PM (3 years, 8 months ago)

From what I've heard cotton is not the best option... Maybe u can find micropore tape..? Just to be on the safe side...

I have no personal experience so don't take my word for it...

Quote:

hyphae said:
Cotton can a breeding ground for bacteria it absorbs moisture from the air even and once wet it takes forever to dry out. I'm surprised Paul would ever have suggested that since it was considered no filter was superior to cotton even before Paul hit the seen. I call it mycosense.




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Offlinezakzwijn
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Re: my first monotub, will update during progress [Re: Senku]
    #26695026 - 05/25/20 01:31 PM (3 years, 8 months ago)

Something like this? I rummaged through my medicine cabinet and came up with this, it's breathing bandaid, I used it to treat blisters on my Camino to Santiago de Compostela.



Edited by zakzwijn (05/25/20 01:34 PM)


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OfflineSenku
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Re: my first monotub, will update during progress [Re: zakzwijn]
    #26695042 - 05/25/20 01:41 PM (3 years, 8 months ago)

Quote:

zakzwijn said:
Something like this? I rummaged through my medicine cabinet and came up with this, it's breathing bandaid, I used it to treat blisters on my Camino to Santiago de Compostela.






Community approves 3M micropore tape...

Just google it.


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Offlinezakzwijn
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Re: my first monotub, will update during progress [Re: Senku]
    #26706357 - 05/30/20 03:49 AM (3 years, 8 months ago)

I have been thinking and decided to leave this monotub as it is, since it was put into fruiting conditions from the start. I made a second monotub of which I sealed the holes with micropore tape to trap CO2 and moisture. Not 3M, but another manufacturer... they're pretty much the same product. I am leaving this second tub as it is :smile:

I enclosed a picture of what cotton pads I am using. I pull them apart and use half of each to cover the holes. I won't use them again, but at the time of typing there seems to be good FAE and the pads feel and look dry.

Do you all think this looks healthy? We're almost 10 days in since I made the tub. Moisture content looks to be okay too, since I see tiny droplets on the mycelium and on the plastic bag casing. I think I see some knotting going on but as a beginner I'd like to hear some other opinions.

Conditions: room temperature, around 22 degrees centigrade
Ligth: ambient light, natural day/light cycle, no direct sunlight







Edited by zakzwijn (05/30/20 04:07 AM)


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OfflineSenku
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Re: my first monotub, will update during progress [Re: zakzwijn]
    #26706961 - 05/30/20 11:17 AM (3 years, 8 months ago)

To me it looks bit dry...

Let's see what others think.


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Offlinezakzwijn
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Re: my first monotub, will update during progress [Re: Senku]
    #26706975 - 05/30/20 11:25 AM (3 years, 8 months ago)

Thanks for your answer :smile:

I put it into fruiting conditions from the start, so evaporation also set in from the start. It is true that the amount of condense on the walls of the tub is slowly decreasing, but in the corners on the plastic  I see tiny droplets and on the mycelium itself in some spots, too.

It looks a bit dry near the holes, however the teks I have read here recommend not misting until the surface of the mycelium is dry so I refrained myself from doing that for now. I am watching it daily like a hawk so I will see it immediately when it's getting too dry.

I dunked the spawn 20 minutes in water and I prepared the substrate on the 'water-rich' side too. (1 brick 650g coco-coir, 2L verm, 250ml gypsum, 5L boiling water).

edit: I just took a picture and I have good hopes I am seeing some upcoming primordia here.



Edited by zakzwijn (05/30/20 11:57 AM)


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Offlinezakzwijn
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Re: my first monotub, will update during progress [Re: zakzwijn]
    #26714261 - 06/02/20 02:29 PM (3 years, 8 months ago)

Update: pins! It's not entirely clear from the picture but there are a few dozen. They're still small and growing slowly, this is after 2 days, but I'm on top of it and watching them like a hawk. Pinning started the day after I decided to mist. I do not think this is coincidence. Apparently it was too dry like Senku mentioned. Apart from that I went directly to fruiting my substrate is not that thick as well, like little over 2" so I think not enough moisture was the culprit.

I will continue to mist and fan daily / twice a day now, depending on developments.



Edited by zakzwijn (06/02/20 02:38 PM)


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OfflineMycobro420
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Re: my first monotub, will update during progress [Re: zakzwijn]
    #26714306 - 06/02/20 02:47 PM (3 years, 8 months ago)

The only thing I can say to help is next time make sure u cover top with more coir or whatever u use and to not leave exposed grains on top that will lead to contamination an sub looks dry. For proper surface moisture u will see your sub bead like fresh dew on grass. An for beginners to keep humity up u can out crumbled price of wax paper over top of sub to keep humidity on sub substrates surface


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Offlinezakzwijn
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Re: my first monotub, will update during progress [Re: Mycobro420]
    #26714389 - 06/02/20 03:18 PM (3 years, 8 months ago)

Okay thanks, I will experiment around and will do that the next time. Currently I have another tub with proper micropore tape (but no casing layer) this time, 3rd tub will be with a casing layer. I just mixed up my spawn with the substrate and tossed it in. I assumed contamination wouldn't stand a chance because the substrate was pasteurized and I put in quite a lot of spawn.


Edited by zakzwijn (06/02/20 03:48 PM)


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OfflineMycobro420
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Re: my first monotub, will update during progress [Re: zakzwijn]
    #26714621 - 06/02/20 04:39 PM (3 years, 8 months ago)

With exposed grains it's a gamble it will probly be fine. but does need heavy mist if u are using Polly fill the key is tight as u can on bottom holes loose on top holes give fae atleast once a day make sure u get the sub wit beads on it that's important for even pinset


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OfflineMycobro420
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Re: my first monotub, will update during progress [Re: Mycobro420]
    #26714630 - 06/02/20 04:44 PM (3 years, 8 months ago)

An with the beads u can be sure the sub has good humidity an the prims feed on water beads and from fae they will evaporated wen they start to mature


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Offlinezakzwijn
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Re: my first monotub, will update during progress [Re: Mycobro420] * 1
    #26715908 - 06/03/20 06:20 AM (3 years, 8 months ago)

As stated in

https://www.shroomery.org/forums/showflat.php/Number/20307891#20307891

I will look for a balance between tight stuffed bottom holes and FAE. Putting a fan in the room to compensate for tight filled bottom holes is not my personal preference so I'll go for the loose option and mist when needed.

I'll probably use a single cotton pad for the bottom holes and half a cotton pad (one half of a separated cotton pad, see upper images) for the top holes.
I know cotton pads aren't recommended but they're fixed size and thin. I can easily tape a single or double cotton pad to fine-regulate FAE, if necessary.

So far they work fine but I'll keep an eye on them not becoming too wet and contaminated.


Edited by zakzwijn (06/03/20 06:37 AM)


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OfflineMycobro420
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Re: my first monotub, will update during progress [Re: zakzwijn]
    #26716195 - 06/03/20 09:06 AM (3 years, 8 months ago)

Ya sounds good I have pretty big set up going I still fan once or twice a day an put a fan in corner of room on medium pointing towards the wall to get extra air circulating in room mine keep my temps around 72 in room for my bags and bins an if my beads dry up I mist the sides of my bin if there is already pins.an if I take it outta of colonization an there is no beads I mist above sub and let it slowly fall down till I see beads the fan an cap it


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OfflineMycobro420
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Re: my first monotub, will update during progress [Re: Mycobro420]
    #26716199 - 06/03/20 09:07 AM (3 years, 8 months ago)

Wen fruits mature I don't really worried about the beads the mature fruits don't really need them as much as the prims do


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OfflineMycobro420
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Re: my first monotub, will update during progress [Re: Mycobro420]
    #26716203 - 06/03/20 09:09 AM (3 years, 8 months ago)



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Offlinezakzwijn
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Re: my first monotub, will update during progress [Re: Mycobro420]
    #26722251 - 06/05/20 04:05 PM (3 years, 8 months ago)

Thanks for the link, I'll save this one. I experimented around a bit with misting but did mist less than what I see in the link.

This is how my pins look now, looks like a few are ready for harvest since the veil broke. They're not very tall and grow slowly, biggest are around 5cm estimate. But they look healthy. I suspect the reason for them being small is the lack of moisture earlier. Substrate is not that thick, little over 2".

edit: lots of pins which are small and still growing. The shrooms could have been larger but since this is my first tub and I've gone through a SHIT TON of contaminations which is why I decided to build a flowhood, subpar materials and several fuckups such as using a heat mat during colonization, I am pretty satisfied.

I also uploaded the pics from my flowhood project.



Edited by zakzwijn (06/06/20 11:39 AM)


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Offlinezakzwijn
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Re: my first monotub, will update during progress [Re: zakzwijn]
    #26723970 - 06/06/20 11:53 AM (3 years, 8 months ago)

A few days into the ongoing harvest, while misting at least 2 times daily and pins appearing everywhere. Awesome. Only thing I'm worried about are the exposed grains. I'll be watching these closely.





Edited by zakzwijn (06/06/20 11:54 AM)


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OfflineSockadin
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Re: my first monotub, will update during progress [Re: zakzwijn]
    #26723977 - 06/06/20 11:56 AM (3 years, 8 months ago)

If you let them grow you will get a better harvest.


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Offlinezakzwijn
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Re: my first monotub, will update during progress [Re: Sockadin]
    #26723985 - 06/06/20 11:58 AM (3 years, 8 months ago)

I think the issue with this is that the veils start to break at around 4-5cm tops, so then I'm actually forced to harvest to prevent spore forming right? I am misting 2 days daily and I think they're hydrated enough.

Could be lack of moisture in the beginning, substrate/spawn ratio or substrate depth? I used 1 brick of coir (650g) with 5L water, 2L vermiculite and 250ml gypsum. Added little over 3 quart spawn to that. Substrate depth is less than recommended, a bit over 2".

It looks a bit like the one in this thread:

https://www.shroomery.org/forums/showflat.php/Number/19363648

OTOH I've read that a large pinset but smaller fruits can actually give a good yield and are more potent as well, so I guess we're going to find that out :smile:


Edited by zakzwijn (06/06/20 01:00 PM)


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