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OfflineWavebeam
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B+ strain start to finish * 2
    #26693893 - 05/24/20 10:27 PM (3 years, 8 months ago)

So I have some B+ spores coming to me next week and Id like to pick everyones brain about the best tek to start with soore syringes to jars using either popcorn bird seed or rye berries in jars then to monotub.

And links to tek ps using the above modalities is appreciated.


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InvisibleModularMind
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Re: B+ strain start to finish [Re: Wavebeam]
    #26693905 - 05/24/20 10:35 PM (3 years, 8 months ago)

How much have you researched thus far?


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InvisibleRoger Clemency
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Re: B+ strain start to finish [Re: ModularMind]
    #26693927 - 05/24/20 10:59 PM (3 years, 8 months ago)

If you want to grow with grains you germinate spores on agar and then to the grain jars. Or you can do BRF cakes with spore syringes.

There’s a lot of good stuff here. https://www.shroomery.org/forums/showflat.php/Number/24144021


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Offlinejbgtaa
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Re: B+ strain start to finish [Re: Roger Clemency]
    #26693932 - 05/24/20 11:05 PM (3 years, 8 months ago)

I really wouldn’t use grains for a spore syringe. If you’re going to do it regardless of our advice, I’d order some pre sterilized jars from a reputable sponsor that have self healing inoculation ports. I’ve made a ridiculous amount of spawn and never had success with syringe to grain when I used my own grain jars. Only works when I used grow kit jars for whatever reason, but most likely because the way I prep my grains is optimal for agar. I always seemed to introduce contaminants when I used spore syringes on grain jars I made myself. I really can’t pinpoint why exactly I never was able to use syringes on my jars but I can say forsure you should use PF tek if your using a syringe.

If you want a fat yield I’d use half pint BRF jars to pasteurized coir, just to give you best chances of fruits.


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InvisibleApples in Mono
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Re: B+ strain start to finish [Re: Roger Clemency]
    #26693936 - 05/24/20 11:09 PM (3 years, 8 months ago)

If you wanna maximize odds of success with spore solution to whole grain jars, you're probably best off choosing one of the non-popcorn options and doing pint jars instead of quarts, imo.

And I'd stay away from pre-prepped vendor jars


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OfflineWavebeam
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Re: B+ strain start to finish [Re: jbgtaa]
    #26693942 - 05/24/20 11:15 PM (3 years, 8 months ago)

Im not interested in brf cakes Im more interested in monotub from grain spawn


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OfflineWavebeam
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Re: B+ strain start to finish [Re: ModularMind]
    #26693946 - 05/24/20 11:20 PM (3 years, 8 months ago)

Ive been growing go,den teachers and APEs from grain spawn jars to monotub. I did some grain to grain with wbs to popcorn then to monotub and I am plagued with contamination. Like every tub git contamed. Im nit sure why but I suspect its my misting technique.

Anyway, Im the kind of guy that likes to keep it simple. I want to do B+ to grain jars fro  syringe to monotub. Just lime I started with go,den teachers.

I came to really dislike growing APEs just eay too sensitive and prone to contam.


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InvisibleModularMind
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Re: B+ strain start to finish [Re: Wavebeam]
    #26693948 - 05/24/20 11:21 PM (3 years, 8 months ago)

So you have a game plan with requires tools?


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OfflineWavebeam
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Re: B+ strain start to finish [Re: Apples in Mono]
    #26693950 - 05/24/20 11:22 PM (3 years, 8 months ago)

Would a half filled quart jar work instead of a pint jar?


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Offlinejbgtaa
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Re: B+ strain start to finish [Re: Wavebeam]
    #26693951 - 05/24/20 11:23 PM (3 years, 8 months ago)

Quote:

Apples in Mono said:
If you wanna maximize odds of success with spore solution to whole grain jars, you're probably best off choosing one of the non-popcorn options and doing pint jars instead of quarts, imo.

And I'd stay away from pre-prepped vendor jars



I would normally not suggest pre prepped jars from anyone but for some reason I’ve only ever had syringe success with vendor jars. I guess I prep my jars too wet for added syringe solution. That’s the only reason I’m suggesting them.

My real suggestion is just to use BRF and either fruit them in a shotgun or spawn them to pasteurized coir. Basically just trying to help OP reduce the chances of growing a tub full of mold with no fruits lol.

If he’s gonna use grain he should probably go with super dry wild birdseed or millet, I would think. And affix a self healing port to an sfd. If I was gonna use a syringe for inoculation for my first grow with self made jars I definitely wouldn’t try to prep my own jars and noc in a sab. Not having experience makes him more liable to introduce nasties, considering he has no sterile technique practice.

Quote:

Wavebeam said:
Im not interested in brf cakes Im more interested in monotub from grain spawn



You can spawn brf spawn to a monotub. If you want to do grain to bulk your best chances are going to be using agar to grain to bulk. If you wanna cut corners then you’re going to have to accept that your likely hood of failure is going to increase. Pretty much everyone here is going to advise you to not go syringe to grain. There’s just a lot of risk involved there.

If you have a pressure cooker then go ahead and do what you want. If you’ve already decided what you’re gonna do then what was the point of even asking 🙄

Also yeah use a 2/3 filled qt jar. If you have done any reading you’d know that you’re not supposed to fill grain jars all the way because you should shake them at 30% colonization.


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Edited by jbgtaa (05/24/20 11:25 PM)


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InvisibleModularMind
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Re: B+ strain start to finish [Re: Wavebeam]
    #26693956 - 05/24/20 11:24 PM (3 years, 8 months ago)

Work agar? :shrug:
Do it right.


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OfflineWavebeam
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Re: B+ strain start to finish [Re: jbgtaa]
    #26693957 - 05/24/20 11:25 PM (3 years, 8 months ago)

Ive always had success with syringe to jars. The contam happens in the tub.


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OfflineWavebeam
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Re: B+ strain start to finish [Re: ModularMind]
    #26693959 - 05/24/20 11:26 PM (3 years, 8 months ago)

Innearly made myself bald from pulling my hair with the contam in agar dishes.


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Offlinejbgtaa
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Re: B+ strain start to finish [Re: Wavebeam] * 1
    #26693964 - 05/24/20 11:29 PM (3 years, 8 months ago)

Quote:

ModularMind said:
Work agar? :shrug:
Do it right.



:whathesaid:

You’re gonna waste more time cutting corners and getting no results rather than if you just took the time to do it right. Agar to grain actually is the simplest way to do things given it’s success rate vs syringe to grain. Just sayin :shrug:

Quote:

Wavebeam said:
Ive always had success with syringe to jars. The contam happens in the tub.



No. The contam happens from either bad spawn or from introducing contams to it, due to not practicing sterile technique.

Quote:

Wavebeam said:
Innearly made myself bald from pulling my hair with the contam in agar dishes.



It’s not the agars fault. You need to learn how to use a still air box correctly. Most of us here use one with nearly 100% success rates. Reverting to a less clean and reliable method isn’t going to help anything.

Why do you think they’re called trusted cultivators? Because the teks they put out are consistent and reliable, but only if you follow them exactly. Do you have a still air box?


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Edited by jbgtaa (05/24/20 11:31 PM)


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Offlinejbgtaa
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Re: B+ strain start to finish [Re: jbgtaa]
    #26693974 - 05/24/20 11:35 PM (3 years, 8 months ago)

Upon inspection of your gallery you have had 1 successfully fruited tub. And now you can’t replicate it. That says you got lucky, not that you can successfully use only syringes and grain every time. Check mine or anyone else’s in this threads gallery and see what kind of consistent and amazing results we get from following teks and not cutting corners.


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InvisibleModularMind
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Re: B+ strain start to finish [Re: Wavebeam]
    #26693975 - 05/24/20 11:36 PM (3 years, 8 months ago)

Quote:

Wavebeam said:
Innearly made myself bald from pulling my hair with the contam in agar dishes.




Agar is where contamination is sorted out. It’s why it’s used.
Finding it there is preferable to amongst your knotting substrate.


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OfflineWavebeam
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Re: B+ strain start to finish [Re: jbgtaa]
    #26693993 - 05/25/20 12:03 AM (3 years, 8 months ago)

Ive only pisted pics of one. Ive had several successes. It wasnt till I started doing APEs from Grain to grain when Ive gotten contam problems. In hindsight one major mistake was scaling up so quickly. I git cocky with my firs APE grown and went straight to grain ti grai  making 30 g2g jars lol.


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Offlinejbgtaa
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Re: B+ strain start to finish [Re: Wavebeam]
    #26693997 - 05/25/20 12:12 AM (3 years, 8 months ago)

Yeah g2ging MS grain spawn isn’t always the best idea especially if you don’t have like perfectly healthy spawn.

I really would suggest grabbing a pressure cooker and still air box, along with agar supplies or pre poured plates. If that’s not possible, keep using syringes but go to BRF and then spawn the brf jars to bulk. There’s a few teks on super low budget and simple brf to bulk teks that use syringes and no pressure cooker or agar, I’m pretty sure. Good luck!! 👍


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InvisibleApples in Mono
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Re: B+ strain start to finish [Re: Wavebeam] * 1
    #26694000 - 05/25/20 12:14 AM (3 years, 8 months ago)

You can fit a bunch more pint jars in a PC than you could with less-full quarts, while minimizing colonization time, which will minimize the window for contaminants to take hold. Granted, you'd end up doing more inoculations, which means opportunities to introduce contaminants. But I'd still say that's outweighed by the benefits. You also won't lose as much spawn per contaminated jar. Plus you'll get more practice with inoculation. Then you could spawn to shoeboxes, that way the bulk colonization period is minimized as well, and as with the smaller jars, you won't lose as much per contaminated tub.

But if I we're gonna skip agar, and had no mushrooms, and I just really wanted some as quickly, easily, and reliably as possible, I'd Noc up some widemouth 1/2 pint BRF jars and put them in a proper SGFC


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Offlinejbgtaa
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Re: B+ strain start to finish [Re: Apples in Mono]
    #26694018 - 05/25/20 12:52 AM (3 years, 8 months ago)

Pints or qts, both have their advantages. Qts are just the hobby standard. You can use either with success as long as you work clean


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