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OfflineIrieLion04
Irie

Registered: 05/05/20
Posts: 15
Last seen: 2 months, 13 days
No pour Agar w/ screw lids Help :)
    #26693399 - 05/24/20 06:44 PM (3 years, 8 months ago)

So I plan on using the no pour agar method but was thinking about blending a few teks (Violets & Alien's Holy Grail in order to fit my needs. I already have some ziplock screw top containers on hand and wanted to clarify a few things when it comes to sterilization.

Mostly be following Violets CONTAINER AGAR DISHES
scroll down and you will see, its dated so thats why i'm asking questions. But she using same containers as me

Agar containers would be unmodified....

After agar is poured and lids are screwed on basically what is half way. I would then proceed to wrap tops with foil and stack in my PC on top of some metal quart rings so they are above water.

Then would PC for 40min? Some people say only need 30 because agar can be delicate

Then let PC cool to 0PSI and leave them in for an hour before removing and letting them cool in room temp in order to reduce condensation. Ofcourse when removing from PC I would tighten lids so no contam air gets inside.


Does this sound good as far as sterilization goes?


--------------------
Irie Vybz


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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 1,670
Re: No pour Agar w/ screw lids Help :) [Re: IrieLion04] * 2
    #26693500 - 05/24/20 07:20 PM (3 years, 8 months ago)

That all sounds fine. 20 minutes is plenty.
When you're tired of not being able to see very well, you can do the same thing with glass plates.


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OfflineIrieLion04
Irie

Registered: 05/05/20
Posts: 15
Last seen: 2 months, 13 days
Re: No pour Agar w/ screw lids Help :) [Re: LadysKnight]
    #26695683 - 05/25/20 08:12 PM (3 years, 8 months ago)

cool yea i figure i would hold off on the glass purchase until i got things down


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Irie Vybz


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