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InvisibleThe Fresh PrintsS
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Re: Can't inoculate agar from syringe [Re: MH5109]
    #26693844 - 05/24/20 09:59 PM (3 years, 8 months ago)

I waited almost 2 weeks for ps. Cyan to germ on agar from a syringe.


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OfflinePsyche delics
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Re: Can't inoculate agar from syringe [Re: The Fresh Prints] * 1
    #26693888 - 05/24/20 10:25 PM (3 years, 8 months ago)

Do you send a drop through to cool the needle before actually inoculating it. The heat be killing of the spores. I had this happen once and I'm pretty sure this is why.


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InvisibleTedsDead
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Re: Can't inoculate agar from syringe [Re: Psyche delics] * 1
    #26693917 - 05/24/20 10:47 PM (3 years, 8 months ago)

:whathesaid:

gotta do that


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Offlinejbgtaa
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Re: Can't inoculate agar from syringe [Re: TedsDead]
    #26693933 - 05/24/20 11:08 PM (3 years, 8 months ago)

Yeah you need to make sure the needle is fully cooled before inoculation.

Besides that I’d say just buy prints instead of syringes :shrug: in my experience it’s way easier to get good germination and growth when streaking a print, check out c10s method of streaking spore prints onto agar.


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OfflineLogicaL ChaosM
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Re: Can't inoculate agar from syringe [Re: TedsDead]
    #26693963 - 05/24/20 11:29 PM (3 years, 8 months ago)

Flame-sterilizing before going to agar seems counterproductive to me. I would just use Iso Alcohol on the tip and thats it.


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Offlinejbgtaa
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Re: Can't inoculate agar from syringe [Re: LogicaL Chaos] * 1
    #26693980 - 05/24/20 11:40 PM (3 years, 8 months ago)

Honestly if you’ve kept your syringes capped and they were made cleanly you don’t need to do any of it 🤷🏻‍♂️ The point of agar is to separate clean growth from contams, not to have perfect growth on t0

It’s definitely best practice to try and get healthy germination on the first plate tho. That said, we don’t flame sterilize spores from prints. Just the vessel to move them. I stream my plates with pre-sterilized scalpel blades and have great results. Uses way less plates in the long run, not having to transfer away from contams a bunch of times that I introduced from any number of places or movements


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InvisibleTedsDead
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Re: Can't inoculate agar from syringe [Re: LogicaL Chaos] * 1
    #26694002 - 05/25/20 12:21 AM (3 years, 8 months ago)

Quote:

LogicaL Chaos said:
Flame-sterilizing before going to agar seems counterproductive to me. I would just use Iso Alcohol on the tip and thats it.





if its a fresh tip... sounds like they've used it a few times now


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
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Offlinepoisoned
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Re: Can't inoculate agar from syringe [Re: TedsDead]
    #26694031 - 05/25/20 01:13 AM (3 years, 8 months ago)

If you're doing cakes, you may as well clone some nice looking fruits to agar.


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OfflineNightPuma1
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Re: Can't inoculate agar from syringe [Re: poisoned]
    #26694271 - 05/25/20 06:06 AM (3 years, 8 months ago)

Everyone is writing about flame sterilizing the needle. I do this and let it cool down.

Still haven't gotten any petri growth (including contaminants) 🤷🏼‍♂️


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OfflinePMBastian
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Re: Can't inoculate agar from syringe [Re: NightPuma1]
    #26694304 - 05/25/20 06:34 AM (3 years, 8 months ago)

That happened to me a while ago. A GT syringe I know is good (cause it worked straight to grain) just refused to germinate on agar. Must be 2 months ago now and the plates still show zero growth.

Sadly I don't know why tho, so I don't have any tips for you!


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OfflineAtomHeart
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Re: Can't inoculate agar from syringe [Re: PMBastian]
    #26694330 - 05/25/20 06:55 AM (3 years, 8 months ago)

Can you tell us what agar recipe you are using?


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Offlinepoisoned
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Re: Can't inoculate agar from syringe [Re: NightPuma1]
    #26694350 - 05/25/20 07:12 AM (3 years, 8 months ago)

Quote:

NightPuma1 said:
Everyone is writing about flame sterilizing the needle. I do this and let it cool down.

Still haven't gotten any petri growth (including contaminants) 🤷🏼‍♂️




I usually let a few drops go into the bottom of my SAB before inoculating. Spores die at relatively low temperatures, better cool that needle off for real.


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InvisibleLadysKnight
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Re: Can't inoculate agar from syringe [Re: poisoned]
    #26694386 - 05/25/20 07:41 AM (3 years, 8 months ago)

For some spores that refused to germinate on agar, I've had luck with brf pucks. Then transfer growth to agar.


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Offlinejackrabbitcytoplas
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Re: Can't inoculate agar from syringe [Re: AtomHeart]
    #26694410 - 05/25/20 07:54 AM (3 years, 8 months ago)

I've had no problem germinating on brf/verm and cleaning it up on agar, a single drop in the middle is enough, just make enough of them to have a few good ones.
You can also make shallow brf pucks, wait for them to pin and clone the pins so you are sure you are cleaning a fruiting culture.




Regarding going directly from syringe to agar, search for the word "streaking" in c10's Agar Guide  here https://www.shroomery.org/forums/showflat.php/Number/24330957#24330957


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