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InvisibleHtaeh
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First shoe, little impatient. Cloning question.
    #26693030 - 05/24/20 04:20 PM (3 years, 8 months ago)

So I got a tub, surface conditions weren't met correctly (didn't squish the sides of the sub down at all when spawning)



It's been a while, I want to take the three big un's and fly away.. I know I should wait; can anyone talk me into it or out of it please?

The largest one is the one I'd like to clone, I haven't cloned anything before and my understanding is that I need to cut out a piece of flesh from the inside, inside a sab with a clean plate ready, my question is, if I were to let impatience overwhelm me could I preserve a chunk of flesh in some way until I prepare a plate tomorrow?


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"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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OfflineA.k.aM
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Re: First shoe, little impatient. Cloning question. [Re: Htaeh] * 1
    #26693198 - 05/24/20 05:28 PM (3 years, 8 months ago)

That looks pretty good, surface conditions aren’t related to packing the sides down.

Those big ones will be ready by tomorrow probably so a case could be made for either way, eating now or waiting.

They’ll prob grow a little bit but mostly just the caps will open up.


As far as cloning, you could cut like an inch of stem off and put it in a bag in the fridge til you’re ready.

That should be enough to rip it open and have enough clean tissue in the middle to easily get a piece. If you’re willing to use an extra plate or two to clean it up you could save a tiny piece and drop to to the plate.


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LAGM2020


Edited by A.k.a (05/24/20 05:31 PM)


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InvisibleHtaeh
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Re: First shoe, little impatient. Cloning question. [Re: A.k.a]
    #26693293 - 05/24/20 05:58 PM (3 years, 8 months ago)

Yo A.K.a, thanks for the response.

I read somewhere packing around the edge encourages topside pinning?

Indeed there could, I think I've decided to wait until tomorrow night to trip. Going to let them continue for a few hours as I want to pick them once the veil is more viewable but I don't want to let them tear.

Thanks for the info too, I'll be sure to keep some stem to clone on a plate tomorrow.


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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OfflineInfraredRick
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Re: First shoe, little impatient. Cloning question. [Re: Htaeh]
    #26693414 - 05/24/20 06:51 PM (3 years, 8 months ago)

The best way to minimize side pins is to ensure the surface is correct....level, hydrated, light and fae.


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Inspiration move me brightly.[gradient:#C7C7D4,#CFD4C7]y[/gradient]


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InvisibleHtaeh
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Re: First shoe, little impatient. Cloning question. [Re: InfraredRick]
    #26693510 - 05/24/20 07:24 PM (3 years, 8 months ago)

Quote:

InfraredRick said:
The best way to minimize side pins is to ensure the surface is correct....level, hydrated, light and fae.





Thank you for your input Infrared. Admittedly, I wasn't flying that blind lol, I was happy with everything except the fact I hadn't pushed around the edges down firmer as I read post spawning that it encouraged surface pins.

Also the inconsistency of pinning is probably less to do with conditions and more a result of it being a germination agar plate that nocc'd the grain.


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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