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OfflineMH5109
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Re: Cultivation General Discussion [Re: gizmo1]
    #26691520 - 05/24/20 12:44 AM (3 years, 9 months ago)

Does this AA culture look strange, it looks to puffy to me. Its a fist transfer from spores, I did a biopsy off the spore plate.


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: MH5109]
    #26691524 - 05/24/20 12:53 AM (3 years, 9 months ago)

Quote:

c10h12n2o said:
And aesthetics





yeah thats the only reason

and spores on myc doesnt matter at all





that plate looks fine


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



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Invisiblegizmo1
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Re: Cultivation General Discussion [Re: TedsDead]
    #26691531 - 05/24/20 12:57 AM (3 years, 9 months ago)

Any recommendations on a drip tray for a GH I'm having trouble finding something the right size to use I have it on plastic now but would like to find a tray. The greenhouse is about 34"x14"

Also not sure if I asked before but will this HEPA filter work for a FH
https://www.amazon.com/Novatek-F4012-Metal-Filter-Novair/dp/B01EC608YU/ref=mp_s_a_1_2?dchild=1&keywords=Hepa+24x24+novatek&qid=1590303760&sr=8-2#


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Edited by gizmo1 (05/24/20 01:05 AM)

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OfflineMH5109
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Re: Cultivation General Discussion [Re: TedsDead]
    #26691539 - 05/24/20 01:08 AM (3 years, 9 months ago)

Thanks Tedd, I have had a pretty bad stretch of mean green so I went back to spores and I'm really trying to focus on my sab work. A second opinion never hurts to ease my mind.


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OfflineYeetusdeetus
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Re: Cultivation General Discussion [Re: gizmo1]
    #26691566 - 05/24/20 01:34 AM (3 years, 9 months ago)

Quote:

Psyche delics said:
The inside should be pretty clean just be extra cautious cause your working around bacteria.



Quote:

gizmo1 said:
Quote:

Yeetusdeetus said:
Got a quart of almost fully colonized oats but it’s lookin pretty bacterial. If I were to case and fruit it and then clone a fruit to agar would I have to worry about bacteria or is it more or less sterile inside like a normal fruit?



That's how I got a clean melmac culture it was bacterial as fuck. I got a single fruit, cloned it did a couple tranfers then put it to LC.




:rasta::tongue2:


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Offlinejbgtaa
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Re: Cultivation General Discussion [Re: gizmo1]
    #26691570 - 05/24/20 01:40 AM (3 years, 9 months ago)

Quote:

gizmo1 said:
Any recommendations on a drip tray for a GH I'm having trouble finding something the right size to use I have it on plastic now but would like to find a tray. The greenhouse is about 34"x14"

Also not sure if I asked before but will this HEPA filter work for a FH
https://www.amazon.com/Novatek-F4012-Metal-Filter-Novair/dp/B01EC608YU/ref=mp_s_a_1_2?dchild=1&keywords=Hepa+24x24+novatek&qid=1590303760&sr=8-2#



Try a couple of those plastic trays that people put dog/cat food and water on to keep it off the floor


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: jbgtaa]
    #26692022 - 05/24/20 07:56 AM (3 years, 9 months ago)

Yeah or at Walmart yesterday I saw a tub that was like an underbed but long instead of square. Around 1.5’x4’ that might cover all the dripping spots. Even just the lid upside down.


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OfflineArmchairCalvinist
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Re: Cultivation General Discussion [Re: A.k.a]
    #26692526 - 05/24/20 12:04 PM (3 years, 9 months ago)

I posted some pics of this a few pages back-- was wondering if anyone has ever seen growth like this? All my plates look the same. No contaminants in any plate and they seem to be growing well


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: ArmchairCalvinist]
    #26692561 - 05/24/20 12:23 PM (3 years, 9 months ago)

the slimy stuff on the 1st plate looks like some kind of bacteria riding along with the myc.


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

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Offlinetedoro
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26692578 - 05/24/20 12:34 PM (3 years, 9 months ago)

Quote:

Caps McGee said:
knowing what an appropriate amount is as a noob is near impossible without having drowned and dried a few out




On this topic... I am noticing with my unmodified low profile tubs that while I keep a nice water beading on the surface... the underlying coir is bone dry. Its like the mycelia keeps the water off the substrate. And fruits are forming first where drips from the lid or walls soak the coir. (2.75q to a brick of coir... no drips on squeezing, surface misted with 2/3 cup of water, 1/4 inch pseudo coir casing, very leaky snapped shut lid)



This wasn't misted. Kinda think my lid is too leaky... and its making the mycelia grow too fast. making tons of water drops, but also the coir dries out.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

Edited by tedoro (05/24/20 12:38 PM)

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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: tedoro] * 1
    #26692595 - 05/24/20 12:44 PM (3 years, 9 months ago)

You’re growing quite a bit of mold there it looks like.

That’s probably why it pinned there.


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Edited by A.k.a (05/24/20 12:44 PM)

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Offlinetedoro
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Re: Cultivation General Discussion [Re: A.k.a]
    #26692603 - 05/24/20 12:47 PM (3 years, 9 months ago)

Quote:

A.k.a said:
You’re growing quite a bit of mold there it looks like.

That’s probably why it pinned there.




I let it go 12 hrs too long... the pooling. Its just bacteria. no mold. My damn lids are super downward depressed... I have to keep after it.

But my point is still.... my coir is bone dry, and the fruits are craving wetter coir. (bad photo example... all I had)


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

Edited by tedoro (05/24/20 12:50 PM)

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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: tedoro]
    #26692613 - 05/24/20 12:53 PM (3 years, 9 months ago)

have you tried bottom watering?


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

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Offlinetedoro
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Re: Cultivation General Discussion [Re: TedsDead]
    #26692642 - 05/24/20 01:07 PM (3 years, 9 months ago)

Quote:

TedsDead said:
have you tried bottom watering?




I have, I'm noticing that it takes up nearly nothing unless it want to take it up, which seems to be midway through a flush. (I measure water going in and out)


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Clean spawn thread | Put a thermometer on your PC

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OfflineSockadin
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Re: Cultivation General Discussion [Re: tedoro]
    #26692650 - 05/24/20 01:10 PM (3 years, 9 months ago)

Yes you have a bactiera problem. But even if your coir is bone dry your tub isn't. I would increase your PAE and watch that tub. I suspect the pinning isn't from evaporation and is more likely pinning due to contamination.

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Offlinetedoro
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Re: Cultivation General Discussion [Re: tedoro]
    #26692652 - 05/24/20 01:11 PM (3 years, 9 months ago)

Here is a better photo of the droplets, yet dry coir underneath.

I'm beginning to think my straight to fruiting conditions are too airy, and the coir gets dried out before the mycelia can start to manage the conditions itself.



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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

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Offlinetedoro
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Re: Cultivation General Discussion [Re: Sockadin]
    #26692661 - 05/24/20 01:13 PM (3 years, 9 months ago)

Quote:

Sockadin said:
Yes you have a bactiera problem. But even if your coir is bone dry your tub isn't. I would increase your PAE and watch that tub. I suspect the pinning isn't from evaporation and is more likely pinning due to contamination.




Passive Air Exchange? with a unmodified 28 quart sterilite tub... how do you mean?


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

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OfflineOvoidhunter
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Re: Cultivation General Discussion [Re: tedoro]
    #26692665 - 05/24/20 01:15 PM (3 years, 9 months ago)

Is it correct to colonize substrate completely sealed off from any FAE?


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Invisiblegizmo1
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Re: Cultivation General Discussion [Re: Ovoidhunter]
    #26692672 - 05/24/20 01:17 PM (3 years, 9 months ago)

Quote:

Ovoidhunter said:
Is it correct to colonize substrate completely sealed off from any FAE?



Not a good idea.


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Offlinetedoro
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Re: Cultivation General Discussion [Re: Ovoidhunter]
    #26692673 - 05/24/20 01:17 PM (3 years, 9 months ago)

Quote:

Ovoidhunter said:
Is it correct to colonize substrate completely sealed off from any FAE?




no, that is old old technique. I am simply bringing up that I might be over doing it.


--------------------
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

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