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OfflineActionjunkie
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Agar agar, then what?
    #26692608 - 05/24/20 12:49 PM (3 years, 8 months ago)

So I've been working with agar for couple years now. I just came to the realization that I have no f****** idea what I'm doing! Now I put in time reading trying different things. I know how to make Patsy plates five different ways. I know how to make dextrose and malt agar agars (or agar agar, agars, or is "agar". I sometimes use the pour tek when I get drunk and have a bottle. I watch every video there is on the net ( holy hyperbole Batman) about monoculture, sectoring isolation. And they all give slightly different info. My clones are close to 100% clean by the third transfer (I think).

But I look at two cultures that look totally different, but all clean mycillium. But all I do is emulate what I see in pics and video. I understand all Im doing is giving them more room space to continue growing. And I read threads saying that pic 1 looks and then another saying that pic 2 also looks good. To me they look nothing alike. What are the attributes I should look for? Why? Or is it totally random? Or no one knows and I should document everything from spore to fruit?

To make a long question short, haaa.

Anyone know a book on mycillium explained? Lol

Thanks guy

wash your hands, do it now, while you're thinking about it. What do you have to lose?  NBC


J



--------------------
Consuming mushrooms may not be addictive but growing them is!


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OfflineMachiavelliavore
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Re: Agar agar, then what? [Re: Actionjunkie] * 1
    #26692654 - 05/24/20 01:12 PM (3 years, 8 months ago)

Different media or different genetics could make them look different.  It really doesn't matter how they look as long as they're clean, it only matters what results you get.

I don't think you need to keep notes unless you're fruiting them all.  Just take notes in your labeling.  For me it will be like:
Spore plate:
Hydralisk variety
each sample becomes
Hydra 1
Hydra 2
Hydra 3
...
Hydra n
then as I sample those
each sample of Hydra 1 becomes,
Hydra 1,1 / Hydra 1,2 / ... / Hydra 1,n
every sample of Hydra 1,1 is labeled Hydra 1,1,1 / Hydra 1,1,2 / ... / Hydra 1,1,n
and so on an so forth.  I do this to try and avoid testing the same culture twice.  Usually once I see a plate is under 6 sectors or so I just let it sit and fruit invitro on the plate, then clone that and try it cause I don't have time to test 60 cultures or whatever I'd end up with.

If you fruit all your isolates record yields, then potency test only the ones that yield at whatever benchmark you're looking for.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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