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OfflineDendrocopos
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Registered: 11/02/21
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #27743645 - 04/20/22 01:15 PM (1 year, 9 months ago)

Don't cut corners dude. Just use agar. Different kinds of thickeners than agar Are digested by mycelium. I am not sure what's in that one, but it would probably get less and less solid as the myc would consume it.


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OfflineGarryJarcia
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Re: SEMI-SOLID LIQUID CULTURE [Re: Dendrocopos]
    #27743921 - 04/20/22 04:28 PM (1 year, 9 months ago)

Nice write up! Trying this this week.


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OfflineLukeTheDuke187
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Re: SEMI-SOLID LIQUID CULTURE [Re: GarryJarcia]
    #27744806 - 04/21/22 07:45 AM (1 year, 9 months ago)

I tried to use the agar but when I do it seems to just make a ring of solid LC at the bottom and the majority of it is liquid still. Any suggestions?


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OfflineDendrocopos
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Registered: 11/02/21
Posts: 511
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #27744809 - 04/21/22 07:47 AM (1 year, 9 months ago)

Use unmoded lids. Put a marble inside. Shake the fuck out of it. Maybe make the water hot when adding the agar.


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InvisibleDoctor Mario
πŸ„πŸ„πŸ„πŸ„πŸ„
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Re: SEMI-SOLID LIQUID CULTURE [Re: LukeTheDuke187]
    #27744814 - 04/21/22 07:49 AM (1 year, 9 months ago)

Quote:

LukeTheDuke187 said:
I tried to use the agar but when I do it seems to just make a ring of solid LC at the bottom and the majority of it is liquid still. Any suggestions?




Probably using too much agar. Mine doesn't do that.


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OfflineLukeTheDuke187
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Re: SEMI-SOLID LIQUID CULTURE [Re: Doctor Mario]
    #27744818 - 04/21/22 07:53 AM (1 year, 9 months ago)

i used .4g for 250mL


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Invisiblenatedawgnow
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Re: SEMI-SOLID LIQUID CULTURE [Re: LukeTheDuke187]
    #27744841 - 04/21/22 08:10 AM (1 year, 9 months ago)

Boil your agar solution before pcing it to make sure all of the agar
is melted. Sometimes that happens if you just mix the agar and cold water then pc immediately.

All the agar sinks, melts in The pc cycle and then re solidifies as a puck at the bottom.


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OfflineLukeTheDuke187
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #27744843 - 04/21/22 08:14 AM (1 year, 9 months ago)

Ah gotcha, thanks. What I did was use an electric tea kettle and put boiling water into a mason jar and added the agar. Do you think it didnt get melted enough?


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OfflineHongosamongus
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #27745999 - 04/21/22 11:46 PM (1 year, 9 months ago)

Has anybody tried to use Semi-solid liquid culture for Paneaolus spp., or is this a no-go?


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I'm a stranger here myself.


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Offlinekarri0n
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Re: SEMI-SOLID LIQUID CULTURE [Re: Hongosamongus]
    #27750037 - 04/24/22 10:00 PM (1 year, 9 months ago)

Quote:

Hongosamongus said:
Has anybody tried to use Semi-solid liquid culture for Paneaolus spp., or is this a no-go?




It's probably better. Pan myceliumn is wispier than cubensis and less robust after a shake, and this protects the myc.


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Panaeolus Bisporus


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OfflineCrackatoa
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Re: SEMI-SOLID LIQUID CULTURE [Re: karri0n] * 1
    #27750080 - 04/24/22 11:18 PM (1 year, 9 months ago)

Pan Cyan Rainbow Eucalyptus fruits


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Crack's Tek's and Shit



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Offlinehazyhorse
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Re: SEMI-SOLID LIQUID CULTURE [Re: Crackatoa]
    #27750109 - 04/25/22 12:18 AM (1 year, 8 months ago)

this is badass nate!! thanks for the write up. definitely saving this thread to try in future grows


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you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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OfflineCatsLoveHouseMusic
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Re: SEMI-SOLID LIQUID CULTURE [Re: hazyhorse]
    #27789394 - 05/23/22 11:59 AM (1 year, 8 months ago)

So I was thinking about trying this as my first LC experience, do you guys suggest this tek over others or what do you think?

I’m doing an experiment. Half jars with quality SHIP/Syringe Filters and half unmodded in 500ml media jars. Should the unmodded ones be like 1/3-1/2 full? And when PCing the unmodded ones, you loosen the cap and use tin foil as usual, like with agar?


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Offlinekarri0n
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Re: SEMI-SOLID LIQUID CULTURE [Re: CatsLoveHouseMusic]
    #27789688 - 05/23/22 05:06 PM (1 year, 8 months ago)

Does SSLC ever get crystal clear the way a fully colonized broth gets?

I like that particular indicator in case of bacteria, and so far all of my SSLC mixes have stayed pretty turbid.


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Panaeolus Bisporus


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OfflineCrackatoa
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Re: SEMI-SOLID LIQUID CULTURE [Re: karri0n]
    #27789837 - 05/23/22 07:07 PM (1 year, 8 months ago)

I love this tek over any other LC recipes. I would suggest testing before using like any LC but the cloudiness makes it harder to read from the naked eye.
I only ever make 100ml-200ml of LC I knock up plenty with that and G2G from there if I want to. Unmodded just leave a little loose, cover with foil,PC and I tighten before even getting it out of the PC.


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Crack's Tek's and Shit



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Offlineholybrown101
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Re: SEMI-SOLID LIQUID CULTURE [Re: Crackatoa]
    #27789874 - 05/23/22 07:35 PM (1 year, 8 months ago)

no filter you say....
I'm knocking up 2 jars today but with jello, i have agar but wanted to try something different. Will post results. CANT WAIT


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OfflineCrackatoa
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Re: SEMI-SOLID LIQUID CULTURE [Re: holybrown101]
    #27789885 - 05/23/22 07:42 PM (1 year, 8 months ago)

Quote:

holybrown101 said:
no filter you say....
I'm knocking up 2 jars today but with jello, i have agar but wanted to try something different. Will post results. CANT WAIT



:llamastare::pondering:


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Crack's Tek's and Shit



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OfflineCatsLoveHouseMusic
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Re: SEMI-SOLID LIQUID CULTURE [Re: Crackatoa]
    #27790120 - 05/24/22 12:59 AM (1 year, 8 months ago)

Do you guys use .5G agar/250ml water? It says .2% nutes as well, but how much nutes do you use? I’ll be using LME.
Anyone add peptone and think it’s worth it?


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Offlinekarri0n
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Re: SEMI-SOLID LIQUID CULTURE [Re: CatsLoveHouseMusic]
    #27790765 - 05/24/22 02:56 PM (1 year, 8 months ago)

Quote:

CatsLoveHouseMusic said:
Do you guys use .5G agar/250ml water? It says .2% nutes as well, but how much nutes do you use? I’ll be using LME.
Anyone add peptone and think it’s worth it?




I followed the recipe exactly - 0.5g to 250ml the first time and ended up with a solid block.

I shook it and it's like the consistency of grape jelly now, a bunch of liquidy blobs and some chunks of solid agar in there. Not ideal, but it colonized ok.

I think depending on your brand of agar you will need to play with the ratios. 0.35 was better for me.


I also had one batch end up with a solid platter of agar on the bottom and liquid above. I know now to make sure to swirl it as it cools, and not let it cool to solid in the PC.

Everyone has a different ratio of nutrients they like in LC but from everything I've seen it looks like you can go realllllly low nutrients and still get a good amount of mycelium in a broth. 0.2% LME should be fine as would 0.1 or 0.5 or 0.05

I've never heard of peptone in an LC broth. Maybe in agar, but even then it doesn't seem necessary for any of the active species I've seen people cultivate or cultivated myself.


--------------------

Panaeolus Bisporus


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InvisibleMurphSmurf
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Registered: 02/08/18
Posts: 68
Re: SEMI-SOLID LIQUID CULTURE [Re: Crackatoa]
    #27790783 - 05/24/22 03:14 PM (1 year, 8 months ago)

Quote:

Crackatoa said:
Pan Cyan Rainbow Eucalyptus fruits





Bro ... hell yeah!


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Long-time lurker - love this site - love to learn - love agar - MMFam


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