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Dendrocopos
Latin woodpecker



Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 1 year, 7 months
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#27743645 - 04/20/22 01:15 PM (1 year, 9 months ago) |
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Don't cut corners dude. Just use agar. Different kinds of thickeners than agar Are digested by mycelium. I am not sure what's in that one, but it would probably get less and less solid as the myc would consume it.
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GarryJarcia
(~);}


Registered: 03/09/22
Posts: 106
Last seen: 6 days, 8 hours
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Re: SEMI-SOLID LIQUID CULTURE [Re: Dendrocopos]
#27743921 - 04/20/22 04:28 PM (1 year, 9 months ago) |
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Nice write up! Trying this this week.
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LukeTheDuke187
Stranger

Registered: 08/10/20
Posts: 163
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Re: SEMI-SOLID LIQUID CULTURE [Re: GarryJarcia]
#27744806 - 04/21/22 07:45 AM (1 year, 9 months ago) |
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I tried to use the agar but when I do it seems to just make a ring of solid LC at the bottom and the majority of it is liquid still. Any suggestions?
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Dendrocopos
Latin woodpecker



Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 1 year, 7 months
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#27744809 - 04/21/22 07:47 AM (1 year, 9 months ago) |
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Use unmoded lids. Put a marble inside. Shake the fuck out of it. Maybe make the water hot when adding the agar.
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Doctor Mario
πππππ



Registered: 08/07/20
Posts: 1,894
Loc: πΊπΈ
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Quote:
LukeTheDuke187 said: I tried to use the agar but when I do it seems to just make a ring of solid LC at the bottom and the majority of it is liquid still. Any suggestions?
Probably using too much agar. Mine doesn't do that.
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LukeTheDuke187
Stranger

Registered: 08/10/20
Posts: 163
Last seen: 10 hours, 21 minutes
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i used .4g for 250mL
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Boil your agar solution before pcing it to make sure all of the agar is melted. Sometimes that happens if you just mix the agar and cold water then pc immediately.
All the agar sinks, melts in The pc cycle and then re solidifies as a puck at the bottom.
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LukeTheDuke187
Stranger

Registered: 08/10/20
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#27744843 - 04/21/22 08:14 AM (1 year, 9 months ago) |
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Ah gotcha, thanks. What I did was use an electric tea kettle and put boiling water into a mason jar and added the agar. Do you think it didnt get melted enough?
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Hongosamongus
Stranger
Registered: 03/04/22
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Loc: Dry
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#27745999 - 04/21/22 11:46 PM (1 year, 9 months ago) |
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Has anybody tried to use Semi-solid liquid culture for Paneaolus spp., or is this a no-go?
-------------------- I'm a stranger here myself.
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karri0n
Mind Traveller



Registered: 08/29/20
Posts: 698
Last seen: 15 days, 9 hours
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Quote:
Hongosamongus said: Has anybody tried to use Semi-solid liquid culture for Paneaolus spp., or is this a no-go?
It's probably better. Pan myceliumn is wispier than cubensis and less robust after a shake, and this protects the myc.
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Panaeolus Bisporus
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
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Re: SEMI-SOLID LIQUID CULTURE [Re: karri0n] 1
#27750080 - 04/24/22 11:18 PM (1 year, 9 months ago) |
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Pan Cyan Rainbow Eucalyptus fruits
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 17 hours, 16 minutes
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Re: SEMI-SOLID LIQUID CULTURE [Re: Crackatoa]
#27750109 - 04/25/22 12:18 AM (1 year, 8 months ago) |
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this is badass nate!! thanks for the write up. definitely saving this thread to try in future grows
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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CatsLoveHouseMusic
SpeakerFreaker



Registered: 11/03/18
Posts: 1,085
Last seen: 1 year, 7 months
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Re: SEMI-SOLID LIQUID CULTURE [Re: hazyhorse]
#27789394 - 05/23/22 11:59 AM (1 year, 8 months ago) |
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So I was thinking about trying this as my first LC experience, do you guys suggest this tek over others or what do you think?
Iβm doing an experiment. Half jars with quality SHIP/Syringe Filters and half unmodded in 500ml media jars. Should the unmodded ones be like 1/3-1/2 full? And when PCing the unmodded ones, you loosen the cap and use tin foil as usual, like with agar?
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karri0n
Mind Traveller



Registered: 08/29/20
Posts: 698
Last seen: 15 days, 9 hours
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Does SSLC ever get crystal clear the way a fully colonized broth gets?
I like that particular indicator in case of bacteria, and so far all of my SSLC mixes have stayed pretty turbid.
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Panaeolus Bisporus
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 2 hours, 44 minutes
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Re: SEMI-SOLID LIQUID CULTURE [Re: karri0n]
#27789837 - 05/23/22 07:07 PM (1 year, 8 months ago) |
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I love this tek over any other LC recipes. I would suggest testing before using like any LC but the cloudiness makes it harder to read from the naked eye. I only ever make 100ml-200ml of LC I knock up plenty with that and G2G from there if I want to. Unmodded just leave a little loose, cover with foil,PC and I tighten before even getting it out of the PC.
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holybrown101
Stranger

Registered: 11/01/09
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Re: SEMI-SOLID LIQUID CULTURE [Re: Crackatoa]
#27789874 - 05/23/22 07:35 PM (1 year, 8 months ago) |
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no filter you say.... I'm knocking up 2 jars today but with jello, i have agar but wanted to try something different. Will post results. CANT WAIT
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 2 hours, 44 minutes
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Quote:
holybrown101 said: no filter you say.... I'm knocking up 2 jars today but with jello, i have agar but wanted to try something different. Will post results. CANT WAIT

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CatsLoveHouseMusic
SpeakerFreaker



Registered: 11/03/18
Posts: 1,085
Last seen: 1 year, 7 months
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Re: SEMI-SOLID LIQUID CULTURE [Re: Crackatoa]
#27790120 - 05/24/22 12:59 AM (1 year, 8 months ago) |
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Do you guys use .5G agar/250ml water? It says .2% nutes as well, but how much nutes do you use? Iβll be using LME. Anyone add peptone and think itβs worth it?
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karri0n
Mind Traveller



Registered: 08/29/20
Posts: 698
Last seen: 15 days, 9 hours
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Quote:
CatsLoveHouseMusic said: Do you guys use .5G agar/250ml water? It says .2% nutes as well, but how much nutes do you use? Iβll be using LME. Anyone add peptone and think itβs worth it?
I followed the recipe exactly - 0.5g to 250ml the first time and ended up with a solid block.
I shook it and it's like the consistency of grape jelly now, a bunch of liquidy blobs and some chunks of solid agar in there. Not ideal, but it colonized ok.
I think depending on your brand of agar you will need to play with the ratios. 0.35 was better for me.
I also had one batch end up with a solid platter of agar on the bottom and liquid above. I know now to make sure to swirl it as it cools, and not let it cool to solid in the PC.
Everyone has a different ratio of nutrients they like in LC but from everything I've seen it looks like you can go realllllly low nutrients and still get a good amount of mycelium in a broth. 0.2% LME should be fine as would 0.1 or 0.5 or 0.05
I've never heard of peptone in an LC broth. Maybe in agar, but even then it doesn't seem necessary for any of the active species I've seen people cultivate or cultivated myself.
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Panaeolus Bisporus
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MurphSmurf
Float On



Registered: 02/08/18
Posts: 68
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Re: SEMI-SOLID LIQUID CULTURE [Re: Crackatoa]
#27790783 - 05/24/22 03:14 PM (1 year, 8 months ago) |
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Quote:
Crackatoa said: Pan Cyan Rainbow Eucalyptus fruits

Bro ... hell yeah!
-------------------- Long-time lurker - love this site - love to learn - love agar - MMFam
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