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OfflineOne Love
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Registered: 07/02/12
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Re: SEMI-SOLID LIQUID CULTURE [Re: ShroomerInTheRye] * 1
    #27342948 - 06/10/21 11:18 AM (2 years, 7 months ago)

Haha just posted this but actually I kind of did this sslc by accident. Had only my agar premix on hand (agar
Peptone dextrose), made these little vials and noticed they were already jelling...so I dumped out half out of them and replaced it with water instead before pcing
Now I'm left with some sslc vials and the results are looking great
Imo! Better than my honey vials. Bout two days in.
At ten MLs it probably won't take long before jI can try using these. Overall I feel going slightly solid was a happy accident. Seems to keep the myc spread out but still moveable and it can still be sucked up with syringe.


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Offlinegone-pear-shaped
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #27437873 - 08/21/21 11:14 PM (2 years, 5 months ago)

Do you have any guess about a diagnosis for SSLC that isn't viscous when it pours? It could be:
  • Colonized too long
  • Agar "structure" got broken too much by shear, on a stir plate
  • Too much agar
  • Too little agar

Side note: it would be interesting to make SSLC from carbomer (acrylic thickener like in hand sanitizer or hair gel). It would be perfectly clear and shear would not break it up like agar can be broken up. Gelatin also supposedly reforms its bonds after being broken up, unlike agar.


Edited by gone-pear-shaped (08/21/21 11:53 PM)


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OfflineLostkeys
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Re: SEMI-SOLID LIQUID CULTURE [Re: gone-pear-shaped]
    #27472610 - 09/18/21 06:16 AM (2 years, 4 months ago)

Prepared a media bottle with .1% LME and .2% agar. Will be inoculating this evening and will post results.


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Invisiblenatedawgnow
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Re: SEMI-SOLID LIQUID CULTURE [Re: Lostkeys]
    #27475370 - 09/20/21 08:54 AM (2 years, 4 months ago)

Nice how did it go? See a lot of people adding agar to lcs lately :rockon:


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OfflineLostkeys
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #27475516 - 09/20/21 10:55 AM (2 years, 4 months ago)

Quote:

natedawgnow said:
Nice how did it go? See a lot of people adding agar to lcs lately :rockon:



It's colonizing


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OfflineDharmaForKarma
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #27590479 - 12/22/21 09:50 AM (2 years, 1 month ago)

Hey LC fans

It's been over a year since this thread was bumped. How's it going with SSLC? Is this technique still producing good results?  Anyone have observations or innovations to add to the discussion?


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OfflineDERRAYLD
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Re: SEMI-SOLID LIQUID CULTURE [Re: DharmaForKarma]
    #27590484 - 12/22/21 09:54 AM (2 years, 1 month ago)

Still works amazingly well.... nothing more to add.


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Invisiblenatedawgnow
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Re: SEMI-SOLID LIQUID CULTURE [Re: DERRAYLD]
    #27590494 - 12/22/21 10:07 AM (2 years, 1 month ago)

This thread was bumped 3 months ago! Give it a shot and feel free to post your results here


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OfflineDharmaForKarma
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #27590519 - 12/22/21 10:29 AM (2 years, 1 month ago)

Quote:

natedawgnow said:
This thread was bumped 3 months ago! Give it a shot and feel free to post your results here




Jeez…2020, 2021. It feels the same.

Thanks!  I made a batch last night with the recipe from memory. 4g agar per liter, right?  🀒

Making a new batch right now, with the actual recipe.


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Invisiblenatedawgnow
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Re: SEMI-SOLID LIQUID CULTURE [Re: DharmaForKarma]
    #27590530 - 12/22/21 10:42 AM (2 years, 1 month ago)

Haha ya 4g per L is double the amount! Good luck on the new batch hope it works for ya!


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Invisiblenix21
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #27706667 - 03/24/22 09:33 AM (1 year, 10 months ago)

I'm a bit late on this, but i fucking love it.. made 16 jars with 200ml white rabbit clone SSLC. Jars  got hard shake after inoculation. First sign of recovery were in 24h.. great stuff, i dont see myself going back to regular LC again after one try on this

Day 4


Shake on day 5

Day 7


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OfflineDendrocopos
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #27724639 - 04/07/22 02:00 PM (1 year, 9 months ago)

Hey dawg. How do you mix these up? I usually put some bolts in my lc and kinda swirl it around. It's kinda hard to do this with sslc. Also, i didn't notice what kinda lids u using. I thought about just using unmoded plastic lids and agetating the lc by besting the shit out of it by banging in up and down.


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Invisiblenatedawgnow
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Re: SEMI-SOLID LIQUID CULTURE [Re: Dendrocopos]
    #27724910 - 04/07/22 07:09 PM (1 year, 9 months ago)

They swirl just fine if your sslc isnt so thick that the whole thing
solidifies but I also use media bottles with unmodded cranked down lids so I shake em as well.


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OfflineDendrocopos
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #27724918 - 04/07/22 07:16 PM (1 year, 9 months ago)

Damn i knew I should've gone unmoded, but i didn't wanna choke it. Polyfil with LC makes me nervous. The consistency is fucking fascinating though, haha. I will report back with results. Thanks for the Tek !


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OfflineSingularFusion
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Registered: 10/31/18
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Re: SEMI-SOLID LIQUID CULTURE [Re: Dendrocopos]
    #27725079 - 04/07/22 09:30 PM (1 year, 9 months ago)

I too have been playing with this fellas

I just used the leftover 30-50ml from pouring plates and topped it back up to 500ml, leaving me with around roughly 0.15% agar, 0.2% nutrition in my bottle I figure

As my agar included yeast, clarity of the SSLC was especially a disaster, so visual inspection for cleanliness is completely out with this method. I guess the agar also fucks with the clarity, depending on exactly which agar. But LC should be tested properly on plates anyway and this just makes you have to do that, instead of taking a shortcut going straight to grain with a broth that is visually clear of any turbidity

The myc really seems to like the colonizing in this more solid structure than in a more watery solution, and when it is spun it seems to recover in a very robust fashion quickly afterwards. With normal LC after vigorous spinning or shaking it seems the myc is almost harmed to some small degree, not so with SSLC I found

So the test on plates proved clean and I will use a little when the time is right and report back with results. I don't make much LC because I am a hobbyist level guy and aspirating LC into syringes from an open bottle in an SAB will test anyone's nerve. But when I do, it may very well be SSLC from here on in

Thanks again for the writeup Nate :cheers:


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OfflineDanny_k
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Registered: 04/06/22
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Re: SEMI-SOLID LIQUID CULTURE [Re: SingularFusion]
    #27725278 - 04/08/22 06:04 AM (1 year, 9 months ago)

This is awesome! I never would have thought to just toss in a small amount of agar into my LC. PROPS!


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InvisibleDoctor Mario
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Re: SEMI-SOLID LIQUID CULTURE [Re: Danny_k] * 2
    #27725364 - 04/08/22 08:01 AM (1 year, 9 months ago)

Just stopping in to say, :thanx: I've been using strictly SSLC for 1 year now and I don't even remember what it's like to a2g or g2g. I think that I use a bit more SSLC in my grain than most people but with 7-10 day colonization, who cares?

I drop a piece of colonized agar to 100mL of SSLC and use 25g of colonized SSLC to inoculate 1 mycoquart (600mL) of grain. The grain is usually fully colonized in 7-9 days depending on the speed of the culture and recovers from a shake another 4 or 5 days later and then gets spawned.

SSLC is better than traditional LC IMO because you get even colonization through out the jar and don't have to shake the jars to distribute colonized pieces of grain.

I have friends who agree that .2% agar is too much because it makes their media too thick but that percentage will probably change with the different agar used. IIRC, they are using telephone. For what it's worth, I've been using Living Jin agar powder since day 1 and haven't had any issues.

If I don't shake the jars regularly, the myc starts to colonize the surface and will turn into a thick puck that's is a pain in the ass to break up. I free pour my media and when a chunk of agar gets to the lip of the jar, media drips and gets all over the place. I use to use magnetic stirrers but I got tired of losing my stir bars to the grain jars when they got low.




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Invisiblefungusul
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Re: SEMI-SOLID LIQUID CULTURE [Re: Doctor Mario]
    #27726356 - 04/08/22 09:54 PM (1 year, 9 months ago)

:takingnotes:


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OfflineDendrocopos
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #27726360 - 04/08/22 09:56 PM (1 year, 9 months ago)

Dang, everyone is unmoded. I really need to get my ADHD under control. I read about one sentence of the Tek and immediately went for it. I think I am gonna repour. Any kind of lc is a bitch with polyfil.


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OfflineLukeTheDuke187
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Re: SEMI-SOLID LIQUID CULTURE [Re: Dendrocopos]
    #27743641 - 04/20/22 01:12 PM (1 year, 9 months ago)



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