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One Love
Surrealist



Registered: 07/02/12
Posts: 297
Loc: Gotham
Last seen: 9 days, 13 hours
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Haha just posted this but actually I kind of did this sslc by accident. Had only my agar premix on hand (agar Peptone dextrose), made these little vials and noticed they were already jelling...so I dumped out half out of them and replaced it with water instead before pcing Now I'm left with some sslc vials and the results are looking great Imo! Better than my honey vials. Bout two days in. At ten MLs it probably won't take long before jI can try using these. Overall I feel going slightly solid was a happy accident. Seems to keep the myc spread out but still moveable and it can still be sucked up with syringe.
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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 5 months, 16 days
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#27437873 - 08/21/21 11:14 PM (2 years, 5 months ago) |
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Do you have any guess about a diagnosis for SSLC that isn't viscous when it pours? It could be:
- Colonized too long
- Agar "structure" got broken too much by shear, on a stir plate
- Too much agar
- Too little agar
Side note: it would be interesting to make SSLC from carbomer (acrylic thickener like in hand sanitizer or hair gel). It would be perfectly clear and shear would not break it up like agar can be broken up. Gelatin also supposedly reforms its bonds after being broken up, unlike agar.
Edited by gone-pear-shaped (08/21/21 11:53 PM)
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Lostkeys
Fractal Thought Pattern



Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 2 months, 4 days
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Prepared a media bottle with .1% LME and .2% agar. Will be inoculating this evening and will post results.
-------------------- So heavy I fell through the earth...

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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: SEMI-SOLID LIQUID CULTURE [Re: Lostkeys]
#27475370 - 09/20/21 08:54 AM (2 years, 4 months ago) |
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Nice how did it go? See a lot of people adding agar to lcs lately
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Lostkeys
Fractal Thought Pattern



Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 2 months, 4 days
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#27475516 - 09/20/21 10:55 AM (2 years, 4 months ago) |
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Quote:
natedawgnow said: Nice how did it go? See a lot of people adding agar to lcs lately 
It's colonizing
-------------------- So heavy I fell through the earth...

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DharmaForKarma
Tub monkey


Registered: 04/24/20
Posts: 565
Last seen: 1 day, 11 hours
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#27590479 - 12/22/21 09:50 AM (2 years, 1 month ago) |
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Hey LC fans
It's been over a year since this thread was bumped. How's it going with SSLC? Is this technique still producing good results? Anyone have observations or innovations to add to the discussion?
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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 9,284
Loc: South Africa
Last seen: 7 hours, 30 minutes
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Still works amazingly well.... nothing more to add.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: SEMI-SOLID LIQUID CULTURE [Re: DERRAYLD]
#27590494 - 12/22/21 10:07 AM (2 years, 1 month ago) |
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This thread was bumped 3 months ago! Give it a shot and feel free to post your results here
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DharmaForKarma
Tub monkey


Registered: 04/24/20
Posts: 565
Last seen: 1 day, 11 hours
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#27590519 - 12/22/21 10:29 AM (2 years, 1 month ago) |
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Quote:
natedawgnow said: This thread was bumped 3 months ago! Give it a shot and feel free to post your results here
Jeezβ¦2020, 2021. It feels the same.
Thanks! I made a batch last night with the recipe from memory. 4g agar per liter, right? π€’
Making a new batch right now, with the actual recipe.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Haha ya 4g per L is double the amount! Good luck on the new batch hope it works for ya!
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nix21
Stranger



Registered: 05/29/18
Posts: 622
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#27706667 - 03/24/22 09:33 AM (1 year, 10 months ago) |
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I'm a bit late on this, but i fucking love it.. made 16 jars with 200ml white rabbit clone SSLC. Jars got hard shake after inoculation. First sign of recovery were in 24h.. great stuff, i dont see myself going back to regular LC again after one try on this
Day 4

Shake on day 5
Day 7
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Dendrocopos
Latin woodpecker



Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 1 year, 7 months
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#27724639 - 04/07/22 02:00 PM (1 year, 9 months ago) |
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Hey dawg. How do you mix these up? I usually put some bolts in my lc and kinda swirl it around. It's kinda hard to do this with sslc. Also, i didn't notice what kinda lids u using. I thought about just using unmoded plastic lids and agetating the lc by besting the shit out of it by banging in up and down.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: SEMI-SOLID LIQUID CULTURE [Re: Dendrocopos]
#27724910 - 04/07/22 07:09 PM (1 year, 9 months ago) |
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They swirl just fine if your sslc isnt so thick that the whole thing solidifies but I also use media bottles with unmodded cranked down lids so I shake em as well.
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Dendrocopos
Latin woodpecker



Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#27724918 - 04/07/22 07:16 PM (1 year, 9 months ago) |
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Damn i knew I should've gone unmoded, but i didn't wanna choke it. Polyfil with LC makes me nervous. The consistency is fucking fascinating though, haha. I will report back with results. Thanks for the Tek !
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SingularFusion


Registered: 10/31/18
Posts: 1,208
Last seen: 9 hours, 47 minutes
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Re: SEMI-SOLID LIQUID CULTURE [Re: Dendrocopos]
#27725079 - 04/07/22 09:30 PM (1 year, 9 months ago) |
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I too have been playing with this fellas
I just used the leftover 30-50ml from pouring plates and topped it back up to 500ml, leaving me with around roughly 0.15% agar, 0.2% nutrition in my bottle I figure
As my agar included yeast, clarity of the SSLC was especially a disaster, so visual inspection for cleanliness is completely out with this method. I guess the agar also fucks with the clarity, depending on exactly which agar. But LC should be tested properly on plates anyway and this just makes you have to do that, instead of taking a shortcut going straight to grain with a broth that is visually clear of any turbidity
The myc really seems to like the colonizing in this more solid structure than in a more watery solution, and when it is spun it seems to recover in a very robust fashion quickly afterwards. With normal LC after vigorous spinning or shaking it seems the myc is almost harmed to some small degree, not so with SSLC I found
So the test on plates proved clean and I will use a little when the time is right and report back with results. I don't make much LC because I am a hobbyist level guy and aspirating LC into syringes from an open bottle in an SAB will test anyone's nerve. But when I do, it may very well be SSLC from here on in
Thanks again for the writeup Nate
-------------------- π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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Danny_k
Explorer

Registered: 04/06/22
Posts: 7
Last seen: 1 year, 3 months
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This is awesome! I never would have thought to just toss in a small amount of agar into my LC. PROPS!
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Doctor Mario
πππππ



Registered: 08/07/20
Posts: 1,894
Loc: πΊπΈ
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Re: SEMI-SOLID LIQUID CULTURE [Re: Danny_k] 2
#27725364 - 04/08/22 08:01 AM (1 year, 9 months ago) |
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Just stopping in to say, I've been using strictly SSLC for 1 year now and I don't even remember what it's like to a2g or g2g. I think that I use a bit more SSLC in my grain than most people but with 7-10 day colonization, who cares?
I drop a piece of colonized agar to 100mL of SSLC and use 25g of colonized SSLC to inoculate 1 mycoquart (600mL) of grain. The grain is usually fully colonized in 7-9 days depending on the speed of the culture and recovers from a shake another 4 or 5 days later and then gets spawned.
SSLC is better than traditional LC IMO because you get even colonization through out the jar and don't have to shake the jars to distribute colonized pieces of grain.
I have friends who agree that .2% agar is too much because it makes their media too thick but that percentage will probably change with the different agar used. IIRC, they are using telephone. For what it's worth, I've been using Living Jin agar powder since day 1 and haven't had any issues.
If I don't shake the jars regularly, the myc starts to colonize the surface and will turn into a thick puck that's is a pain in the ass to break up. I free pour my media and when a chunk of agar gets to the lip of the jar, media drips and gets all over the place. I use to use magnetic stirrers but I got tired of losing my stir bars to the grain jars when they got low.

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fungusul
Fungus Kingdom


Registered: 07/16/20
Posts: 1,028
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Dendrocopos
Latin woodpecker



Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 1 year, 7 months
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#27726360 - 04/08/22 09:56 PM (1 year, 9 months ago) |
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Dang, everyone is unmoded. I really need to get my ADHD under control. I read about one sentence of the Tek and immediately went for it. I think I am gonna repour. Any kind of lc is a bitch with polyfil.
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LukeTheDuke187
Stranger

Registered: 08/10/20
Posts: 163
Last seen: 8 hours, 11 minutes
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Re: SEMI-SOLID LIQUID CULTURE [Re: Dendrocopos]
#27743641 - 04/20/22 01:12 PM (1 year, 9 months ago) |
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