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InvisibleZifozonke
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Re: SEMI-SOLID LIQUID CULTURE [Re: eatyualive]
    #26692587 - 05/24/20 12:40 PM (3 years, 8 months ago)

I like the sound of this...gonna give this one a go thanks nate!!


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Invisiblenatedawgnow
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Re: SEMI-SOLID LIQUID CULTURE [Re: Zifozonke]
    #26692803 - 05/24/20 02:31 PM (3 years, 8 months ago)

Let us know how it goes!


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OfflineA.k.aM
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #26701644 - 05/28/20 11:43 AM (3 years, 7 months ago)

I made one of these with natalensis the other day.

Did you notice if it took a little longer to colonize??

I’m feeling good about this one, the consistency is like chocolate syrup almost. Or watered down maple syrup.

I made a couple with different amounts of agar, the first one ended up solid lol.


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LAGM2020


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Invisiblenatedawgnow
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Re: SEMI-SOLID LIQUID CULTURE [Re: A.k.a]
    #26701647 - 05/28/20 11:45 AM (3 years, 7 months ago)

No it colonized at the same rate as the other but the myc is more difficult
to see because of the opaqueness of the agar


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OfflineA.k.aM
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #26701661 - 05/28/20 11:50 AM (3 years, 7 months ago)

Awesome.

I’ll probably pour Sunday or Monday and update.

This could definitely catch on, it’s the simplest and fastest method to get li which I’ve only done with ultra soft plates a couple times but it works great.


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LAGM2020


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Invisiblenatedawgnow
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Re: SEMI-SOLID LIQUID CULTURE [Re: A.k.a]
    #26701672 - 05/28/20 11:53 AM (3 years, 7 months ago)

I like it purely for beimg able to shake the shit out of it and not have the inoculant
sink to the bottom of the vessel. To test these on agar, if you feel the need to,
just dip an inoculation loop into the sslc and streak it to a plate.

I haven't used a needle with these but it might be harder than just dipping a loop.


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OfflineA.k.aM
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #26701679 - 05/28/20 11:56 AM (3 years, 7 months ago)

Yeah with lc I usually shake the jars a couple times the first day and once the second day to keep it from pooling.


I used syringes at first but lately I just pour it in.


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LAGM2020


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Offlinepureshrooming
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Re: SEMI-SOLID LIQUID CULTURE [Re: A.k.a]
    #26707100 - 05/30/20 01:02 PM (3 years, 7 months ago)

4 days in from dropping wedges in two jars and biopsy method on another.

Super hard to see the progress - mostly to do with the jars I use - but it’s going!



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OfflineA.k.aM
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Re: SEMI-SOLID LIQUID CULTURE [Re: pureshrooming]
    #26707135 - 05/30/20 01:18 PM (3 years, 7 months ago)

Yeah I’ve been wondering if maybe some food color would make it easier to see how grown it is, I can’t see anything in mine.

Planning on cooking up some jars tomorrow and pouring Monday, mine will be six days at that point.


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Re: SEMI-SOLID LIQUID CULTURE [Re: A.k.a]
    #26707139 - 05/30/20 01:19 PM (3 years, 7 months ago)

Doesn’t mycelium eat the colour after a while?


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LAGM2020


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Invisiblenatedawgnow
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Re: SEMI-SOLID LIQUID CULTURE [Re: A.k.a]
    #26707144 - 05/30/20 01:23 PM (3 years, 7 months ago)

Quote:

A.k.a said:
Yeah I’ve been wondering if maybe some food color would make it easier to see how grown it is, I can’t see anything in mine.

Planning on cooking up some jars tomorrow and pouring Monday, mine will be six days at that point.



Do you have a stir plate? 6 days aint tht long even cor regular lcs. Sslc needs
to be shaken or stirred relatively frequently to break the clumps of mycelium and agar up.


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Offlinepoisoned
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Re: SEMI-SOLID LIQUID CULTURE [Re: staytrippy420]
    #26709119 - 05/31/20 01:14 PM (3 years, 7 months ago)

Quote:

staytrippy420 said:
Doesn’t mycelium eat the colour after a while?



I think that's the idea here. To see how much colour was eaten.


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OfflineA.k.aM
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Re: SEMI-SOLID LIQUID CULTURE [Re: poisoned]
    #26709123 - 05/31/20 01:16 PM (3 years, 7 months ago)

I don’t have a stir plate but I always shake them pretty violently to break the myc off the wedge.

I put in some time with a flashlight angling the jar and I can definitely see the myc floating in there.

There’s also bubbles which makes it tough.

I’ve got big expectations for this thing.


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LAGM2020


Edited by A.k.a (05/31/20 01:17 PM)


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Offlinepureshrooming
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Re: SEMI-SOLID LIQUID CULTURE [Re: A.k.a]
    #26709235 - 05/31/20 02:10 PM (3 years, 7 months ago)

I had the exact same thought on food coloring! Almost dropped some in when I made the jars but felt like I might get laughed off shroomery :grin:

I did a hearty shake earlier and put a flashlight to mine. There is some good progress but I'm going to wait about a week before I put it to grain. I will have 13 tubs of PE going as of today and need to pump the brakes a bit.


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OfflineA.k.aM
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Re: SEMI-SOLID LIQUID CULTURE [Re: pureshrooming]
    #26711453 - 06/01/20 12:54 PM (3 years, 7 months ago)

Alright I just knocked up six quarts of natalensis with this stuff. Hopefully it’s clean.

This variety is super fast and aggressive and apparently pretty contam resistant. I’m assuming there’ll be some fuzziness by morning.

This is the germ plate at 12 days from streaking. I had taken a few wedges 1.5-2 days earlier and it grew over so quick you can hardly tell.




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Invisiblenatedawgnow
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Re: SEMI-SOLID LIQUID CULTURE [Re: A.k.a]
    #26711475 - 06/01/20 01:01 PM (3 years, 7 months ago)

You'll start seeing recovery within 48 hrs if it's clean and full colonization
in 4-5 days if you shake right after pouring


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OfflineA.k.aM
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #26711496 - 06/01/20 01:10 PM (3 years, 7 months ago)

I worked up a sweat shaking those jars. :box:

Lately I’ve noticed much quicker recovery with regular lc.  idk if it’s a coincidence and I’ve just had fast cultures but I switched my recipe and started using them within ten days also and I’ve been seeing 24 hour recovery.

Some of my other lcs that were older would take up to five days sometimes.


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OfflineA.k.aM
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Re: SEMI-SOLID LIQUID CULTURE [Re: A.k.a]
    #26716959 - 06/03/20 02:29 PM (3 years, 7 months ago)

Two days after pouring


This stuffs sticky alright.


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LAGM2020


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InvisibleRoger Clemency
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Re: SEMI-SOLID LIQUID CULTURE [Re: A.k.a]
    #26729279 - 06/08/20 04:07 PM (3 years, 7 months ago)

So i did a blenderless LI 250ml water with .2g agar. I decided not to put anything in the jar to help with the shaking because the blender ball annoyed me the last time but i think it hurt my cause. Things are still going well but a lil slower than what i'm seeing here. Still faster than when i did this without the agar though.

The first 4 jars were only shaken after the pour. The second 4 i shook the next day as well. That seems to have not worked out as well unless by some coincidence all of those jars got a little less than the others. The one least colonized on one side (with the big white wedge growth) was the last to be poured and didn't have much liquid but got a shitload of the agar puck. The center is probably pretty solid.

I've also noticed this PE6 myc seems to be slower in general than the other things i've got going on.

7th day:


A little more light on the 'once shaken' jars:


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Invisiblenatedawgnow
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Re: SEMI-SOLID LIQUID CULTURE [Re: Roger Clemency]
    #26729597 - 06/08/20 06:23 PM (3 years, 7 months ago)

I'm confused, were those done with blenderless LI or sslc?


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