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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
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SEMI-SOLID LIQUID CULTURE 27
#26689470 - 05/23/20 12:54 AM (3 years, 8 months ago) |
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SEMI-SOLID LIQUID CULTURE Hello everybody and welcome to my thread!
So I've been working recently on a novel LC tek for funsies and for science!
I'm calling it semi-solid liquid culture (SSLC). So I noticed that LC tends to not like a rough shake, and also tends to sink to the bottom of the jar/bag until colonized a bit for a shake, only at this point can it withstand the damage from rough shaking. My solution to this problem? Enter SSLC 
The idea is to add a small amount of agar to your LC. I tested a bunch of ratios from .1-.5% and settled on .2% as the best ratio. I used .5g agar for a 250mL liquid culture for this batch. This makes a very viscous soup. Anymore agar and it will solidify completely. The amount of nutrients can be whatever you usually use for LCs. These were made with .2% nutes as well.
For this round I made 2 LCs, one traditional as a control, and one SSLC. So here are the 2 broths pre agar wedge so you can see how they look:
Regular LC

SSLC

Each LC was knocked up with a TOC clone.
Here are some pics a week or so into colonization. Unfortunately the SSLC is kinda hard to see in the photos:
Regular LC

SSLC

Ok so I knocked up some jars with each LCs on the 14th. For each LC I made a a control jar where I just dumped it in and let it sit, a shake jar that I shook vigorously, and a roll jar that I gently rolled back and forth to mix the LC/grains. Each jar got around 10mL of LC, I free poured so impossible to be exact but that's what I was shooting for.
Here are the comparison shots between each LC on day 4. Roll and shake jars are from multiple angles. SSLC jars are on the left:
Control jars:

Roll jars:
 
Shake jars:
 
As you can see the SSLC jars are all almost fully colonized and the SSLC control jar shows a lot more growth than the regular LC. My theory is that the myc has a stronger foothold because of the thicker medium and because of this it can withstand more vigorous shaking and is less likely to sink to the bottom of the jars because it clings to grains. My goal with it was to be able to shake it vigorously starting out to soak grains so that the jar colonizes in days instead of a week+.
As a side experiment, I dumped 50ml of SSLC into 1 jar to see how it will colonize. Here you can see how the SSLC just sits on top of the grains until I shake it:

Here it is at day 4:

The jar is fully colonized, definitely a lot of moisture but no actual pools of water. This jar was shaken right after dumping in the 50ml.
Well that's about all he wrote! You may say "why bother when LC works just fine?"
My answer is because it's a hobby, it's fun, try some new shit every once in a while.
Let me know what you guys and gals think and if you have any comments please share!
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#26689743 - 05/23/20 06:16 AM (3 years, 8 months ago) |
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A.k.a
Stranger



Registered: 10/27/19
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Re: SEMI-SOLID LIQUID CULTURE [Re: eatyualive] 1
#26690050 - 05/23/20 09:47 AM (3 years, 8 months ago) |
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Right on man I did exactly this with plates a while back during li experiments.
It looks like this colonizes much faster than the plates did.
I’ll prob make one of these today myself.
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LAGM2020     
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pureshrooming
feels like a stranger


Registered: 05/28/18
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Re: SEMI-SOLID LIQUID CULTURE [Re: A.k.a]
#26690148 - 05/23/20 11:06 AM (3 years, 8 months ago) |
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Just used up the last of my LC so this is perfect timing! I'll give it a whirl
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
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Feel free to post pics and what not here when you do!
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pureshrooming
feels like a stranger


Registered: 05/28/18
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#26690793 - 05/23/20 04:41 PM (3 years, 8 months ago) |
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Just pulled 2 jars of LC with agar, 1 jar of standard LC, and 2 jars of water to make LI out of the PC. Gonna toss a wedge in all of them from the same clone plate and see who comes out on top! I'll update this when I have some results.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
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Nice what ratios did you do for the sslc?
I found, and should have included im the tek, that with telephone agar it was necessary to pre boil my broth to make sure all the agar dissolved before pcing.
Is yours nice and thick?
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pureshrooming
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#26691321 - 05/23/20 09:55 PM (3 years, 8 months ago) |
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Yep nice and thicc! I do a pre-boil also so I got it nice and clear. It is definitely thicker than I expected but easily shaken.
I did 500g water, 1g agar, and 4g of lme then split that up between jars
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
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Make sure to give it a good swirl every once in a while to mix the colonized myc in. Just don't get your filters wet with it, unless of course you're LC is filterless (way I do it)
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A.k.a
Stranger



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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#26692028 - 05/24/20 08:01 AM (3 years, 8 months ago) |
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I’m going to buy hay and hulls but when I come back I’m getting in on this.
I just can’t decide which type to do.
Probably albino cambo or APEU.
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LAGM2020     
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
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Re: SEMI-SOLID LIQUID CULTURE [Re: A.k.a]
#26692144 - 05/24/20 08:53 AM (3 years, 8 months ago) |
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Nice write-up.
Agar in LC isn't very novel tho
https://www.shroomery.org/forums/showflat.php/Number/13781184/fpart/1/vc/1
And someone else recently made an adding agar to LC tek also saying it helped the LC stick to the grain giving faster recovery. Ill see if I can find the thread it was the same exact concept.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: SEMI-SOLID LIQUID CULTURE [Re: bodhisatta] 6
#26692252 - 05/24/20 09:50 AM (3 years, 8 months ago) |
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If the only reference you can find is from 10 years ago I say it's relatively novel 
Nothing is entirely original anymore, that's just where we are in the evolution of the human mind
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#26692277 - 05/24/20 10:00 AM (3 years, 8 months ago) |
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If you can find that other thread I'd be interested in reading it. I did a quick cursory search from the past 5 years before posting the tek and pulled up nothing similar other than caps' sagari which is what gave me the idea
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#26692291 - 05/24/20 10:07 AM (3 years, 8 months ago) |
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-------------------- Tek's I use LAGM2020
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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How fast of recovery improvement are you seeing? I think this would be great for bag culture. I feel LI and LC seem to seep through the cracks in bags a little more than jars. I’ve recently been getting 4 day colonization with LI using these new pour lids I bought. And this isn’t a variety that is generally fast. I also only did my initial shake after inoculating(#rubbedoneout)
Im also of the mind that the chunkier and thicker liquid inoculant is, the better it sticks to the grain and the faster it colonizes.
A lot of it has to do with the way you shake and roll jars. I usually fill my quarts really high for LI or liquids. I actually did a lower volume of inoculation than I normally do and it colonized in 4 days. 13 out of 19 jars did this. And the ones I didn’t shake quite as well are still at 75-90%. Ive since spawned those 13 quarts. This time I did 1 plate to 19 quarts where I normally do 1 plate to 10.

Edited by eatyualive (05/24/20 10:52 AM)
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LotKid
Never.Trust.A.Prankster


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Re: SEMI-SOLID LIQUID CULTURE [Re: bodhisatta] 9
#26692338 - 05/24/20 10:29 AM (3 years, 8 months ago) |
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Quote:
bodhisatta said: Nice write-up.
Agar in LC isn't very novel tho
https://www.shroomery.org/forums/showflat.php/Number/13781184/fpart/1/vc/1
And someone else recently made an adding agar to LC tek also saying it helped the LC stick to the grain giving faster recovery. Ill see if I can find the thread it was the same exact concept.
Looks to me like someone is unfamilair with the saying... "those who live in glass houses; probably shouldn't throw stones."
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verum subsequentis
seeker of truth



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Re: SEMI-SOLID LIQUID CULTURE [Re: LotKid]
#26692362 - 05/24/20 10:38 AM (3 years, 8 months ago) |
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Nice, Nate!
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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@eat- basically seeing full colonization between 4 and 5 days. Haven't done a bag yet but that is next on the list.
@verum and lot- thanks guys 
"All that is old shall become new through rebirth."
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Edited by natedawgnow (05/24/20 02:05 PM)
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poisoned
untitled



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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#26692491 - 05/24/20 11:48 AM (3 years, 8 months ago) |
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Hah, I just inoculated a small jar of snot consistency agar like a few days ago.
Edited by poisoned (05/24/20 11:49 AM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#26692532 - 05/24/20 12:07 PM (3 years, 8 months ago) |
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Nice! I’d definitely be interested to see your results. I love liquids! 
Hmm snot agar you say....
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