Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
OfflineSiggNature
Fun Gi
Registered: 04/24/20
Posts: 31
Last seen: 3 years, 8 months
2 Pound Bag Colonized in 6 Days After Agar!
    #26689375 - 05/22/20 11:16 PM (3 years, 8 months ago)

I first wanted to say please have patients as I am new to writing on forums, I don’t know how this will look when I am done.

I just wanted to share what happened with my first agar to grain transfer.

The first thing that I did is inoculate a slant without wood with a spore syringe in a SAB and then waited for it to get huge and puffy with mycelium. (At this point I did not have a Flow Hood)
(I actually did 15 tubes and only had 3 that grew mycelium and did not end up with contamination.)

I cut the fastest growing tube up into tiny pieces with sterile procedures in front of my flow hood using a scalpel and then dumped them into my bag of sterilized grain that I cut open in front of the flow hood, then sealed the bag. I then shook the bag up as evenly as I could.

* (I had used RR’s method for preparing the rye grain.)

The bag was stored at 70-82 F

I did not shake the mycelium during the colonization, I did not need to and I believe that it would have set it back if I did.

6 Days later I have a fully colonized bag of rye grain, and I am thrilled!! 😁







Edited by SiggNature (05/22/20 11:29 PM)


Extras: Filter Print Post Top
InvisibleThe Fresh PrintsS
Smell ya later
Male User Gallery


Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air Flag
Trusted Cultivator
Re: 2 Pound Bag Colonized in 6 Days After Agar! [Re: SiggNature]
    #26689386 - 05/22/20 11:27 PM (3 years, 8 months ago)

You basically inoculated a grain bag with a germ plate. You would have had the same success rate (maybe better) by shooting those spores directly into the grain. Its nice that you have spawn, but I think your approach was misguided.


--------------------


Extras: Filter Print Post Top
OfflineSiggNature
Fun Gi
Registered: 04/24/20
Posts: 31
Last seen: 3 years, 8 months
Re: 2 Pound Bag Colonized in 6 Days After Agar! [Re: The Fresh Prints]
    #26689412 - 05/22/20 11:44 PM (3 years, 8 months ago)

I appreciate your observation. I may have had the same results, but the reason I did that is because I was having a lot of trouble with contamination. I think that the spore syringe had a high level of contamination in it. Instead of wasting all of the time preparing grain and waiting for it to be contaminated, I inoculated a bunch of tubes and waited to see which ones would have no contams, then I used that for assurance. I had tried many jars and a few bags before that shooting them with a spore syringe and I was tired of seeing trich. I was still confused about agar at the time too. I am near the beginning of the process of finding a good isolate now with agar plates. No contamination now that I am going agar to agar with a flow hood!


Extras: Filter Print Post Top
InvisibleThe Fresh PrintsS
Smell ya later
Male User Gallery


Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air Flag
Trusted Cultivator
Re: 2 Pound Bag Colonized in 6 Days After Agar! [Re: SiggNature]
    #26689423 - 05/22/20 11:56 PM (3 years, 8 months ago)

The flow hood is a game changer. Sounds like you've got the right idea. You won't know if you have a good isolate until you grow it out.
Take that bag, cross your fingers for some fruits and start playing with clones.


--------------------


Extras: Filter Print Post Top
OfflineSiggNature
Fun Gi
Registered: 04/24/20
Posts: 31
Last seen: 3 years, 8 months
Re: 2 Pound Bag Colonized in 6 Days After Agar! [Re: The Fresh Prints]
    #26689431 - 05/23/20 12:02 AM (3 years, 8 months ago)

It absolutely is! I appreciate your guidance. Btw, is the mycelium in that jar on your signature an isolate?


Extras: Filter Print Post Top
InvisibleThe Fresh PrintsS
Smell ya later
Male User Gallery


Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air Flag
Trusted Cultivator
Re: 2 Pound Bag Colonized in 6 Days After Agar! [Re: SiggNature]
    #26689439 - 05/23/20 12:19 AM (3 years, 8 months ago)

Quote:

SiggNature said:
It absolutely is! I appreciate your guidance. Btw, is the mycelium in that jar on your signature an isolate?



Nah. You don't need an isolate to get growth like that. Sometimes you just get lucky with genetics.


--------------------


Extras: Filter Print Post Top
OfflineSiggNature
Fun Gi
Registered: 04/24/20
Posts: 31
Last seen: 3 years, 8 months
Re: 2 Pound Bag Colonized in 6 Days After Agar! [Re: The Fresh Prints]
    #26689442 - 05/23/20 12:23 AM (3 years, 8 months ago)

Wow, I didn’t know that. So when I grow it out, am I just looking for the biggest one from the flush, or the fastest, or combination or what exactly should I look for to clone?


Extras: Filter Print Post Top
InvisibleDCAE
Base

Registered: 02/21/20
Posts: 10,242
Loc: somewhere in eurasia
Re: 2 Pound Bag Colonized in 6 Days After Agar! [Re: SiggNature]
    #26689469 - 05/23/20 12:51 AM (3 years, 8 months ago)



Extras: Filter Print Post Top
OfflineSiggNature
Fun Gi
Registered: 04/24/20
Posts: 31
Last seen: 3 years, 8 months
Re: 2 Pound Bag Colonized in 6 Days After Agar! [Re: DCAE]
    #26689488 - 05/23/20 01:27 AM (3 years, 8 months ago)

Thank you, that’s exactly what I wanted to know.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Agar Contamination cookiewhore 3,273 1 01/26/02 09:54 PM
by Workman
* I got a 10 pound bag of Pennington Rye Grass Seed Sporz 1,505 2 05/20/02 01:20 PM
by Pinner
* Myco-Bag Colonization Insecurities!?!? Phantastic 1,610 6 06/12/02 05:32 AM
by EcoCrush
* Jar vs Bag Colonization Swami 1,935 4 02/28/02 05:20 PM
by SouthernGent
* Agar contamination PaleTylenol 2,004 3 12/31/01 09:07 AM
by MAIA
* MYCO BAG MIGHT BE CoNTAMINATED JameZTheNewbie 1,512 4 06/30/02 06:40 PM
by JameZTheNewbie
* Is this weilii on agar contaminated? GGreatOne234 2,061 5 09/13/02 08:16 AM
by GGreatOne234
* myco bags colonized!!! diacamomo 896 2 06/30/02 04:04 PM
by TM

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
288 topic views. 7 members, 113 guests and 29 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.007 seconds on 12 queries.