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OfflineLogicaL ChaosM
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Re: Too many burst grains cause bacteria? [Re: Yeetusdeetus]
    #26688491 - 05/22/20 03:37 PM (3 years, 8 months ago)

Quote:

Yeetusdeetus said:
Fuck lol so you think the burst hulls could be my problem?




If your grains busted, then u over-cooked your grains either before PCing or from PCing themselves. Overcooked typically means overhydrated. So yes, burst grains can cause bacteria. Gotta start over.


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Invisiblenatedawgnow
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Re: Too many burst grains cause bacteria? [Re: LogicaL Chaos]
    #26688507 - 05/22/20 03:40 PM (3 years, 8 months ago)

Burst grains don't cause bacteria, bacteria spores cause bacteria. Burst grains are
just a more favorable environment for bacteria when present


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OfflineLogicaL ChaosM
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Re: Too many burst grains cause bacteria? [Re: natedawgnow]
    #26688526 - 05/22/20 03:46 PM (3 years, 8 months ago)

My bad, burst grains can cause bacteria to grow easier but doesnt cause bacteria. Hows that?


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OfflineYeetusdeetus
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Re: Too many burst grains cause bacteria? [Re: A.k.a]
    #26688571 - 05/22/20 04:02 PM (3 years, 8 months ago)

Quote:

bodhisatta said:
What's die really mean for a single celled bacteria when you can get it wet and it puts itself back together. Some of these signals that bacteria make during biofilm formation tell a subset of the film to protect their DNA and even further tell some bacteria to start forming extra tough endospores with more dipicolinic acid. As well some are forming with moderate levels and low levels. So that there's endospores that are super protected from DNA damage and some that are more ready to spring into action. Many people seem to think endospores only form in dire conditions. That just pushes the amount formed to a higher %. Bacteria is always maximize its survival chances. Some subset of the population of a film is making spores at a slow rate during optimal conditions and during dire conditions that proportion grows larger. This is why tyndalization doesn't really work and there's other reasons too like Never being able to germination every spore during the in between sterilization phases anyway.

I like to think of a biofilm in or on a grain as a fortified castle with a modern bank vault inside and inside the vault are people wearing the same gear that the people who work with molten steal do and if they die so long as not all their DNA is burnt to a crisp they can rebuild and come back to life.

So yea much different than sterilizing liquid




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InvisibleHans Wermhat
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Re: Too many burst grains cause bacteria? [Re: LogicaL Chaos]
    #26688695 - 05/22/20 05:01 PM (3 years, 8 months ago)

Quote:

LogicaL Chaos said:
My bad, burst grains can cause bacteria to grow easier but doesnt cause bacteria. Hows that?



Because no matter how careful you are or how many precaution one takes, things are never 100% clean. Take the filter for example in our jar lid. They're rated something like 99.997% efficient at 0.2 micron or something like that? That means that even though they're really good, a tiny tiny amount of bacteria or spore will still pass through, especially on the cooldown when the pressure inside the jar decreases and outside air gets sucked in through the filter to equalize the pressure. Cultures are never 100% perfectly clean, pressure cooker never 100% effective, etc. But that's okay, because 99.9999999% sterility is good enough to allow a healthy culture to fully colonize the grain. Having starchy burst grains can slow mycelium down a little and is a good breeding ground for bacteria, so it tips the scales a little more toward contamination and will exacerbate/exploit any weak point in your sterile technique.


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