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Celestialexplorer1



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Ms syringe str8 to LC tek *UNDELETED* 2
#26686104 - 05/21/20 04:55 PM (3 years, 8 months ago) |
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Ok so I don’t know if this is something someone has tried before. I mean it’s a big world so probably right, but I’ve read through a lot of stuff and never seen it so I figured I would share my results with you. Here goes...
So this post will be about converting a spore syringe to liquid culture without a jar and skipping multiple steps that normally one would take making there liquid culture jar. It’s not for everyone I’m aware and sure you can get a ton more out of your liquid culture jar than you ever would with this method but for those looking for an easy way and less time consuming this may be for you.
This post will also be using a home made panaeolus cyanescens (Jamaican for those that care) spore syringe. So here’s what I did:
What your going to need: Spore syringe Clean needles alcohol lamp Print of your choice Another preferably small container with self seal port Organic corn syrup/dextrose/honey(I like corn syrup and dextrose)

Spent syringe was cleaned with very warm water near boiling. Sucked up and spit out several times into a different cup( in through the clean cup out to a waste cup just to make sure your following) tap water sucked up one last time to about 8ml your going to need at least 2ml extra so don’t overfill.
Syringes we’re wrapped in foil and PCed for 45 mins to kill any possible spore inside both syringes and needles. Also very nice if you have leur lock caps as well. They are necessary.
You need to make your spore syringe with sterile water drawing up with a sterile needle in a sterile glass cup in a sterile environment (sab/flow hood/vacuum of complete nothingness) Ok so I like to use these little vials they already have a sealing port and there really small and cool looking. And they hold just enough of the CS to make a couple of the LC syringes.

Put your organic earth friendly corn syrup into your small container. Put into your pc and sterilize for 20-30 mins depending on the size or your container. It’s important to not cook this too long as you could caramelize your corn syrup. Also beneficial if you only have 16g needles to pre mix with a little and I mean little bit of water or you will not be able to suck this through the needle. 18g is fine it will pull through.
So you’ve pced your corn syrup now take a clean sterile needle and draw up .32 ml of the corn syrup into your spore syringe(if you had 8ml to begin with what your shooting for is 4% syrup or dextrose or honey). And then shake shake shake and shake. Look at it. Is it mixed yet. Shake it some more to be sure. Look again. Ok your done.
Now wait for the magic.
Every now and then give it a real good shaking and don’t hold back. You want tons of little tiny bubbles to move through the liquid aerating it... like this 
This is after just under a day do you see the germinated spores. You might have to squint a bit.
This is panaeolus cyanescens if you missed that little nugget.

Technically if you have ants in your pants you could just use this tek as a jumpstart for your spores before shooting up a bag or jar and go ahead and use it but I would suggest raid... and patience for the ants in your pants. The best things in life take time and hard work to get. It makes the success you achieve so much greater.
Cliff notes: put sterile corn syrup into a sterile spore syringe and wait a bit. You now have a less complicated way to make a LC syringe. Not that the original way is complicated and Just as all things sterile Liquid cultures are very susceptible to contamination and also very hard to tell when it contams.
Day 3 (noticeable size increase of germination) hard to tell in the pictures more to come

The camera doesn’t pick it up well but by the end of day three already a dozen or so little mycelial clumps can be seen.
Day 4

Day 5
At this point I used the solution on a 1/2 pint to test it out
This is 9 days later
Edited by Celestialexplorer1 (05/21/20 09:58 PM)
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Sockadin



Registered: 01/03/10
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Honestly when I read the title and got about 2 sentences in I was thinking to my self... This is gonna be fail sauce, but after a full read. I'm impressed, it isn't the best way to get a clean culture but hell I like it anyway.
This will still fail alot because print to syringe is the breaking point, but I like the idea of letting it colonize the LC in the syringe instead of a larger jar.
Cool project!
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Celestialexplorer1



Registered: 03/25/20
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Re: Ms syringe str8 to LC tek [Re: Sockadin]
#26686306 - 05/21/20 06:32 PM (3 years, 8 months ago) |
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Thanks and yes I totally agree honestly I was surprised too I really didn’t know if it would work. And I could see this going wrong in so many ways but if your clean and follow the sterile guide lines some may pull it off
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Celestialexplorer1



Registered: 03/25/20
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 Honestly the reason I went for the small grain jar was because I had my doubts. I am an avid agar user, it iis so much more assuring. And you can get so much more out of it. But I thought I’d give this a try and just see
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Sockadin



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Why not rewrite the thread and do a demonic agar punch into the back of the syringe and then suck up the sterile corn syrup? Then your not writing a thread with a potential high failure rate that new growers will follow? Just saying... If you don't I might!
Either way it is a cool way to skin the cat, but fruiting isn't sterile and that is always gonna be the point of failure.
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SFS96
AstroMan



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Re: Ms syringe str8 to LC tek [Re: Sockadin]
#26686455 - 05/21/20 07:41 PM (3 years, 8 months ago) |
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Yeah im surprised everyone hasn’t shot this tek to shit yet. Its a cool idea but any time u put spores stait to a LC your taking a big risk. How many times have you tested this? Most spore prints aren’t clean enough to do this Not trying to be a ass or anything but this might point noobs in the wrong direction.
-------------------- How I make and preserve tea
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c10h12n2o
serial dilutor



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Re: Ms syringe str8 to LC tek [Re: SFS96]
#26686501 - 05/21/20 07:57 PM (3 years, 8 months ago) |
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I dont really understand the benefit
Spores are never sterile unless you fruited, harvested, and made syringe in a flow hood on sterilized substrate
This is coming from someone who loves LC
I just dont see the benefit in skipping agar. Lc is already so easy to contam, i wouldnt want to recommend anyone make their odds even worse by using non sterile spores as a starting point
Sure, it can work, but one extra step makes it far more likely to work. Also for such a small volume of LC, i use 3-5x that for one bag
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Celestialexplorer1



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Re: Ms syringe str8 to LC tek [Re: c10h12n2o]
#26686509 - 05/21/20 08:01 PM (3 years, 8 months ago) |
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So question c10 do you use Lc made from agar. Also there are many teks out there telling people to put spore in a cup with water from a sterilized needle, mix it with the tip and suck it. If this is done in front of a flow good or in an SAB which it was, how is this any less sterile. Now I completely agree agar is so much better and it’s virtually all I use now. But seeing all the LC teks our there I don’t feel there any more sterile.
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Edited by Celestialexplorer1 (05/21/20 08:08 PM)
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Celestialexplorer1



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How would I delete this post
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Sockadin



Registered: 01/03/10
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I wouldn't we have all posted shit we look back on and think, "what was I thinking?" It is probably the best example of learning.
I still think your on to something, but I would modify the Tek to use a single agar punch in the back of the syringe. Love the idea of sucking up sterile corn syrup or something else and letting it colonize in the needle.
Let's run with this instead of remove it.
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c10h12n2o
serial dilutor



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Re: Ms syringe str8 to LC tek [Re: Sockadin]
#26686699 - 05/21/20 09:33 PM (3 years, 8 months ago) |
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I wouldnt delete it either, its helpful for others
Agar helps make sure you have clean myc and contams reveal themselves. I personally wouldnt ever put spores on anything but agar. Yes all my lcs are made with agar at least 3 transfers away from the spore streak plate
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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DnDRnD
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Quote:
Celestialexplorer1 said: So question c10 do you use Lc made from agar. Also there are many teks out there telling people to put spore in a cup with water from a sterilized needle, mix it with the tip and suck it.If this is done in front of a flow good or in an SAB which it was, how is this any less sterile.Now I completely agree agar is so much better and it’s virtually all I use now. But seeing all the LC teks our there I don’t feel there any more sterile.
This is less sterile because mushrooms arnt fruited in a sterile environment thus the spores and gills are exposed to the contaminates in the open air which hitch a ride into the syringe along with the spores we want in there. That's why we use agar or go to PF cakes with a spore syringe because we can clean up on agar and BRF/Verm mixture is less likely to contaminate than straight grains
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Celestialexplorer1



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Re: Ms syringe str8 to LC tek [Re: DnDRnD]
#26686736 - 05/21/20 09:50 PM (3 years, 8 months ago) |
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I was saying how is it any less sterile than making a spore syringe or other LC teks.. I understand spores are not sterile, therefor spore syringes are always un sterile. Therefor this tek is un sterile... but how is it any less sterile than other LC teks that use spore as there inoculate is what I asked
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Edited by Celestialexplorer1 (05/21/20 10:01 PM)
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c10h12n2o
serial dilutor



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Because lc teks are sterile
We typically make LC from known clean agar, rather than spores
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Gan
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Because, if they're proper teks, they'll assume the LC is being made with a clean culture that has been cleaned up previously on agar
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Celestialexplorer1



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Ok I’m just saying if you do a google search for LC teks at least 80% are done with the direction of using spores. I completely agree though I am team agar all the way. I’m just saying I suppose there’s a lot of older information on the topic.
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c10h12n2o
serial dilutor



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Yeah theres a lot of outdated info
Dont get me wrong, it can work, i did it successfully several times before i started agar. It just isnt a safe bet
Why dont you combine your strategy with backloaded agar, or mudas blenderless LI tek? That ought to be pretty cool for small scale work
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Celestialexplorer1



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Re: Ms syringe str8 to LC tek [Re: c10h12n2o]
#26686828 - 05/21/20 10:42 PM (3 years, 8 months ago) |
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I’ll get an agar transfer in one tomorrow and see what happens. Outa b interesting at least. I’ll post some pictures
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Celestialexplorer1



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I just ordered some 150ml syringes and I’m going to be trying the Backloaded agar with them
There was a 500ml lite lock syringe but that seems a little too big but for large scale work a 500ml could be used to fill your smaller syringes after the colonization period is complete.
Edited by Celestialexplorer1 (05/22/20 09:37 AM)
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Celestialexplorer1



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For this experiment I aquired several 100ml syringes

A solution of 4% organic corn syrup and purified water was made and the syringes filled to 40ml each(they could have been filled up to 80ml but honestly I didn’t want to get my big boy PC out so I used my small one) both syringes were wrapped like burritos and sterilized for 30 mins at 15 psi.

For this project I am using a gallindoi 50g stone clone transfer and a mexicana jalisco T3

 You can see a little tiny stone that was starting to form on this one
Brand new scalpels used both plates were scraped and backloaded with separate new scalpels in SAB. The syringes were filled with flow hood air to 100ml and shaken vigorously


They will now be left to fully collonize the solution.. I am getting agar punches tomorrow and when I have a good enough transfer I will give that a try. I have a lot of outdoor projects coming up with semilanceata, ovoid, alleni and an attempt with azurescens so I will most likely be using this method with those as well.
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