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silverp
CannaPro

Registered: 03/10/20
Posts: 28
Last seen: 3 years, 2 months
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Contam (mold) issue... ID and help?
#26684212 - 05/20/20 08:10 PM (3 years, 8 months ago) |
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Ive gotten this in 3 straight tubs. At 7 days, 14 days, and then 30. 7-days had a ยผ" layer of CV on top and this showed up. 14-Day looked colonized and ready to fruit, but still hadn't pinned, and I killed it because of the contamination. 30-Day tub was stalled... it looked perfect but wouldn't pin. It got a spot of this contam as I was deciding to start over due not pinning. I killed that one too and the substrate looked great except for the contamination. (another issue I can't figure it is not pinning...)
Been really clean and following good lab rules. The 7-Day contamination made me think it was in the coir from the start..
But I am a bit flummoxed... Any ID of this and any thoughts how to avoid in the future?
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 1 day, 6 hours
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Re: Contam (mold) issue... ID and help? [Re: silverp]
#26684243 - 05/20/20 08:24 PM (3 years, 8 months ago) |
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Make sure your spawn is clean first.
Sounds like itโs not pinning because itโs fighting the trich.
Do you have pictures of the spawn?
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LAGM2020     
Edited by A.k.a (05/20/20 08:25 PM)
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silverp
CannaPro

Registered: 03/10/20
Posts: 28
Last seen: 3 years, 2 months
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Re: Contam (mold) issue... ID and help? [Re: A.k.a]
#26684423 - 05/20/20 10:12 PM (3 years, 8 months ago) |
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I expanded the spawn into more jars, 1 of 6 batches went bad, so maybe the spawn was bad? These are the master jars from the spawn, and a saved Gen2 from the fridge.

And from the fridge, Gen 2.... (Condensation is on the outside)
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Contam (mold) issue... ID and help? [Re: silverp]
#26684649 - 05/21/20 12:32 AM (3 years, 8 months ago) |
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That's a crazy amount of mold. It's localized. Look at your spawn not the coir. If it was in the coir I think it would be more spread out. It's crazy those jars don't look too sketchy or anything. Maybe a little bacterial if I zoom in on the 2nd and the 4th jar. By any chance do you have a pic of the culture used? I'm willing to bet of the masters looked good and the g2g jars failed either it shouldn't have been expanded or else bad sterile Tek. Maybe try going only a2g for awhile and see if your problem clears up. If so you have a idea of what you need to work on.
-------------------- Trade List ๐๐๐ 6 hole Mini Monos
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,842
Loc: In the Gills
Last seen: 12 hours, 5 minutes
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Re: Contam (mold) issue... ID and help? [Re: gizmo1]
#26684875 - 05/21/20 05:25 AM (3 years, 8 months ago) |
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And definitely dump that tub outside somewhere
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Contam (mold) issue... ID and help? [Re: rumfor69]
#26684882 - 05/21/20 05:29 AM (3 years, 8 months ago) |
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dont let the mold spoulate like that if you can avoid it. Try to spot it early
3 tubs getting that bad would mean a terribly high mold spore load for your room.
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" โ Friedrich Nietzsche
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rickyswamps
Bad Apple



Registered: 11/08/18
Posts: 1,192
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Re: Contam (mold) issue... ID and help? [Re: c10h12n2o]
#26684913 - 05/21/20 05:56 AM (3 years, 8 months ago) |
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That tub was definitely contaminated spawn. Or just pure contam.
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silverp
CannaPro

Registered: 03/10/20
Posts: 28
Last seen: 3 years, 2 months
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Re: Contam (mold) issue... ID and help? [Re: rumfor69]
#26686308 - 05/21/20 06:34 PM (3 years, 8 months ago) |
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That entire tub, without touching the inside, bagged in a trash bag, made it to a dumpster!
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silverp
CannaPro

Registered: 03/10/20
Posts: 28
Last seen: 3 years, 2 months
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Re: Contam (mold) issue... ID and help? [Re: gizmo1]
#26686321 - 05/21/20 06:42 PM (3 years, 8 months ago) |
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That entire run is trashed and I started a new batch from spores a couple weeks ago. Jars look great - pure white, no visible contams. Did G2G and everything is colonizing with no signs of any contams....
The last monotubs all shared the same grain run - so hopefully that solves this problem.
The weird one that is hard to figure out is the tub that looked great, fully colonized the substrate, and then did nothing. Just stalled completely when it should have pinned.... After 25 days I saw a quarter sized spot where the surface was getting darker, and by day 30 that was about 3 inches and had evolved into obvious mold (trich)...
I guess the lesson is that if trich is in the spawn, it can keep a good looking substrate from fruiting?
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silverp
CannaPro

Registered: 03/10/20
Posts: 28
Last seen: 3 years, 2 months
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Re: Contam (mold) issue... ID and help? [Re: silverp]
#26686448 - 05/21/20 07:39 PM (3 years, 8 months ago) |
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This is the tub that stalled and then finally started showing trich at 28 days....
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