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Sharp-Inflation
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Last minute green contam on top of cakes
#26683378 - 05/20/20 01:40 PM (3 years, 8 months ago) |
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Hello,
This is the second attempt by a friend. They successfully colonized jars of Cambodian Gold with Vermiculite and BRF. As the jars were nearing completion or fully done, many developed a thin green layer on top only. This layer developed within the last 24 hours.
My friend is considering attempting to salvage the operation. Tonight he plans to take the jars into a sterile airtight box (will be inside box for the whole time) and cut out the infected parts. (Should he stop here and just use the clean parts?) From there take the uninfected parts and put them in a dilute bleach or H202 dunk and then bathe them for a short time in water before breaking them up and planting into a coco coir and vermiculite substrate in a monotub.
Any comments/suggestions are welcome to improve the chance of this actually succeeding.
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Mugnath


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Re: Last minute green contam on top of cakes [Re: Sharp-Inflation]
#26683406 - 05/20/20 01:52 PM (3 years, 8 months ago) |
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How do they receive GE? You should be using a vermiculite barrier at the top when following the PF tek.
Edited by Mugnath (05/20/20 01:52 PM)
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Apples in Mono
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Registered: 09/21/17
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Re: Last minute green contam on top of cakes [Re: Mugnath]
#26683509 - 05/20/20 02:40 PM (3 years, 8 months ago) |
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Ah, yes. The old moldy jar surgery Tek. Advanced level stuff. Not sure if you're quite ready for that yet
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Sharp-Inflation
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Re: Last minute green contam on top of cakes [Re: Apples in Mono]
#26683534 - 05/20/20 02:57 PM (3 years, 8 months ago) |
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Yes, my friend did not do enough research before diving in. Has learned some things after reading more. Will do better but if you have recommendations for a good brf cake to monotub tek I would be all ears.
What is GE?
The surgery will happen regardless. Any advice?
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Apples in Mono
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Re: Last minute green contam on top of cakes [Re: Sharp-Inflation]
#26683562 - 05/20/20 03:08 PM (3 years, 8 months ago) |
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Are you sure it's mold and not just bruising?
GE refers to gas exchange. Normally cakes would have a dry vermiculite layer on top, which would act as a filter, allowing gas exchange while keeping contams out. What do your lids look like? Are they filtered with anything?
Surgery usually isn't a great idea when it comes to molds. Same goes for chemical soaks.
If they're actually moldy, I might try to see if they'd produce a few fruits outside
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Sharp-Inflation
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Re: Last minute green contam on top of cakes [Re: Apples in Mono]
#26683629 - 05/20/20 03:41 PM (3 years, 8 months ago) |
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I plan to use the q-tip method to test whether it is mold or just bruising. They have been knocked around more than the prior batch.
I pierced each lid in the center and inoculated with 1 ml solution from a spore syringe. Then carefully plugged the hole with fresh cotton balls (a small piece slightly in and out of each lid).
I agree that it likely will not work but I want to try. I plan to build the clean box and make it air tight then seal all contaminants in air tight containers before bleaching all surfaces inside the box. Then immediately disposing of the contaminated substance outdoors. From there I plan to follow my prior procedure for a bulk monotub.
Thoughts? Suggestions?
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A.k.a
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Re: Last minute green contam on top of cakes [Re: Sharp-Inflation]
#26683634 - 05/20/20 03:42 PM (3 years, 8 months ago) |
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I don’t see anything
Maybe some very light bruising on the fluff on top but as far as I can tell those are fine.
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LAGM2020     
Edited by A.k.a (05/20/20 03:44 PM)
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Sharp-Inflation
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Re: Last minute green contam on top of cakes [Re: A.k.a]
#26683642 - 05/20/20 03:47 PM (3 years, 8 months ago) |
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So will the q-tip test detect whether it is bruising or contam? I will get some more pictures..
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Sharp-Inflation
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Re: Last minute green contam on top of cakes [Re: Sharp-Inflation]
#26683645 - 05/20/20 03:51 PM (3 years, 8 months ago) |
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Roger Clemency
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Re: Last minute green contam on top of cakes [Re: Sharp-Inflation]
#26683650 - 05/20/20 03:57 PM (3 years, 8 months ago) |
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Since they are fully colonized you can open them right now in open air. No need for a sterile box. As far as chemical soaks the only thing that will do is weaken your mycelium and make matters worse if there is a problem, and create a problem if there isn’t one already.
Your method of inoculating and then putting in an unsterile cotton ball is not the recommended way ; ) It wouldn’t surprise me at all if there was some kind of mold up top cuz the spores have had all this time to fall through, especially if you’ve moved the jars or picked them up.
If I was you I would cut off the moldy part (one jar, 2nd to last, I can pretty clearly see mold 98% sure) and then try to fruit them as cakes. If you break them up and spawn that’s just more energy the myc has to expend in recolonizing substrate and all that time the other bits of mold will also be growing and you’ll likely end up with a mold sub. With those cakes I’d say you at least have a decent chance of a few mushrooms poking up
Edit: I feel like I see some pins as well in there, though it’s hard to tell with that type of glass. If there are pins already you most definitely wanna just let them grow cuz that’s your best chance if mold is around
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Sharp-Inflation
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Re: Last minute green contam on top of cakes [Re: Roger Clemency]
#26683654 - 05/20/20 04:01 PM (3 years, 8 months ago) |
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Do you think this is something that should be done immediately or do I have a few days to ponder the issue?
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A.k.a
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Re: Last minute green contam on top of cakes [Re: Sharp-Inflation]
#26683663 - 05/20/20 04:09 PM (3 years, 8 months ago) |
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Yeah take the lid off and take a picture.
If it’s moldy already it can’t get worse and if not it’s all colonized so it’s safe.
I still think they’re good it looks like bruising.
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LAGM2020     
Edited by A.k.a (05/20/20 04:10 PM)
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Sharp-Inflation
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Re: Last minute green contam on top of cakes [Re: A.k.a]
#26683738 - 05/20/20 04:42 PM (3 years, 8 months ago) |
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A friend performed the qtip test on one of the greenest jars with no effect on the qtip, no green. A friend is starting to believe it is just bruising and will spawn to substrate as usual. In the future will be more careful about moving.
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A.k.a
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Re: Last minute green contam on top of cakes [Re: Sharp-Inflation]
#26683803 - 05/20/20 05:18 PM (3 years, 8 months ago) |
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Cool.
That second jar looks like it might have some bacteria though. First one looks great.
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LAGM2020     
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Sharp-Inflation
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Re: Last minute green contam on top of cakes [Re: A.k.a]
#26683813 - 05/20/20 05:21 PM (3 years, 8 months ago) |
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My friend's plan is to let them remain sealed for a few more days to consolidate. If the contam spreads then that jar will not be used. Otherwise the jars with minimal bruising will be spawned in two-three days.
Does this sound like a good plan?
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A.k.a
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Re: Last minute green contam on top of cakes [Re: Sharp-Inflation]
#26683831 - 05/20/20 05:27 PM (3 years, 8 months ago) |
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They look ready to me but a couple more days can’t hurt.
There’s no serious contam you’ll be fine.
What’s the plan for them?
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LAGM2020     
Edited by A.k.a (05/20/20 05:28 PM)
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Sharp-Inflation
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Re: Last minute green contam on top of cakes [Re: A.k.a]
#26683865 - 05/20/20 05:38 PM (3 years, 8 months ago) |
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Take 10-15 jars of Cambodian Gold and another batch of Golden Teachers are coming along behind them.
Mostly following the linked tek https://www.shroomery.org/forums/showflat.php/Number/15998565/fpart/all/vc/1
Coco coir and verm mix in a monotub according to the above tek. Following to a t (not the jars, but everything from the colonized spawn onwards).
Other post contains a monotub that was recently completed by the same friend.
Edited by Sharp-Inflation (05/20/20 05:39 PM)
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