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Senior-Meow
House cat


Registered: 05/18/20
Posts: 25
Last seen: 4 months, 17 days
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How my plates are doing? (with pics)
#26681316 - 05/19/20 02:48 PM (3 years, 8 months ago) |
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Hello everyone. I'm trying agar work and those plates are the 5th transfer from print. I'm not sure to be on the right track so some feedback will be very useful. In general - are those plates seem healthy to you?



 What get me confused is the different patterns on these plates. Plate 1-3: is that ariel myc? Plate 4 seem the most healthy to my inexperienced eyes; but between 6-8 oclock there's a different pattern of myc. Is it different myc genetic or contam? Any help will be much appreciated.
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WinterOwned
Fighter of the Nightman

Registered: 08/30/08
Posts: 149
Last seen: 1 year, 3 months
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Re: How my plates are doing? (with pics) [Re: Senior-Meow]
#26681398 - 05/19/20 03:43 PM (3 years, 8 months ago) |
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Also inexperienced, but I'm pretty sure the last one is just fine.
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Htaeh
Immortal


Registered: 10/14/18
Posts: 320
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Re: How my plates are doing? (with pics) [Re: Senior-Meow]
#26681447 - 05/19/20 04:08 PM (3 years, 8 months ago) |
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Also inexperienced but I think they're all okay, the last one looking the most aesthetically pleasing. The others although difficult to see, just look tomentose to me, which are still fine. I reckon that the reason I keep getting a lot of tomentose plates recently is due to a high nutrient agar recipe which I have yet to change to see if it differs.
-------------------- "To write is to wage war" ~ Voltaire "The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D. "A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher "We'll know our disinformation program is complete when everything the US public believes is false." ~ William J. Casey, 1981 "Fear always works to influence the population, when the population is uneducated." "The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~ Maximilien Robespierre "Knowledge makes a man unfit to be a slave." ~ Frederick Douglass
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 17 hours
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Re: How my plates are doing? (with pics) [Re: Htaeh]
#26681558 - 05/19/20 05:21 PM (3 years, 8 months ago) |
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Plate 4 that is just different genetics. There are many strains present and the more transfers you make you will start to see the different genetics show as sectors.
The first 3 cultures look like weak myc. What is your agar recipe ?
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (05/20/20 06:00 PM)
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Senior-Meow
House cat


Registered: 05/18/20
Posts: 25
Last seen: 4 months, 17 days
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Re: How my plates are doing? (with pics) [Re: mushpunx]
#26830132 - 07/18/20 03:34 AM (3 years, 6 months ago) |
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I just want to share an update.. After I changed me recipe from BRF to MAE, my plates got on the right track.
My plates from the original post were all clean I believe.. Even had pins growing from those plates eventually I transferred those pins and here are the results:


Looking much better I say, thanks for your help.
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w00tmycelium
Stranger


Registered: 11/28/19
Posts: 159
Last seen: 3 years, 4 months
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Re: How my plates are doing? (with pics) [Re: Senior-Meow]
#26830151 - 07/18/20 04:05 AM (3 years, 6 months ago) |
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Quote:
Senior-Meow said: I just want to share an update.. After I changed me recipe from BRF to MAE, my plates got on the right track.
My plates from the original post were all clean I believe.. Even had pins growing from those plates eventually I transferred those pins and here are the results:

Looking much better I say, thanks for your help.
This second plate looks gorgeous. You are on the right track.
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Senior-Meow
House cat


Registered: 05/18/20
Posts: 25
Last seen: 4 months, 17 days
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Re: How my plates are doing? (with pics) [Re: w00tmycelium]
#26830168 - 07/18/20 04:37 AM (3 years, 6 months ago) |
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Thanks!
It's actually the same plate but my pic taking skills could get better
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w00tmycelium
Stranger


Registered: 11/28/19
Posts: 159
Last seen: 3 years, 4 months
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Re: How my plates are doing? (with pics) [Re: Senior-Meow]
#26830170 - 07/18/20 04:43 AM (3 years, 6 months ago) |
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I've been trying to isolate this down from T2 to T4 currently but yours looks even better than mine. I noticed a few colonies were stronger per plate but it seems better to do 4 transfers per T1 and T2 per plate before going further, but yours are still great!
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jcm4620
Stranger


Registered: 05/26/19
Posts: 6,700
Last seen: 1 year, 11 months
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Re: How my plates are doing? (with pics) [Re: w00tmycelium]
#26830263 - 07/18/20 06:55 AM (3 years, 6 months ago) |
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you should really try to take smaller transfers you will get what your after faster😃😃
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