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Offlinepoisoned
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Poisoned's glass petri no-pour tek. * 6
    #26680235 - 05/19/20 03:29 AM (3 years, 8 months ago)

I see a lot of people on here wanting to use glass dishes, but there's not many teks on them. While glass dishes aren't for everyone, I believe everyone should have at least a couple of them at home as a backup.

You don't need boro dishes to do it. I've been using soda lime glass dishes for quite a long time now and none have cracked in a PC. There's a common misconception that you need boro for any high temperature application, but it actually doesn't matter in situations where you get nice even heating. Your jars are made of soda lime glass and if they're sitting in water during your PC cycle, they will experience way bigger temperature differences than your agar dishes.

Also, many people are cautious about boiloff. I had it happen a few times when some dishes were filled all the way to the brim. Try to keep your agar level at about half height for best results.

So, let's get to work. You'll need:
  • PC
  • Some glass dishes
  • Agar
  • Saran wrap
  • Vinegar


First, make your agar. Use whatever recipe you want. I like to put agar and LME into cold water and let it sit for at least an hour before cooking it. It seems to bring out zero sediment and perfect clarity. Then put it on a stove and cook it until everything is dissolved nicely. Be careful, because it likes to boil over and it's a bitch to clean. Pour it into a pitcher or a bottle or whatever you want to pour with:


Lay out your dishes on the counter like this:


Pour them:


If you're as clumsy as I am, you might have poured some over the edges. Nothing to worry about, you'll just have some extra work.

Let agar cool in the dishes. Once it's solid enough, use a paper towel and clean up any agar that didn't end up in dishes. Make sure to clean edges and bottoms thoroughly.

Now take out your saran wrap and stack dishes onto it like this:


Wrap them:


An important thing to note here is that you want saran on the bottom to be flat, so your dishes will sit level in your PC. You also want only one layer of saran. You'll see why later.

Put your dishes in a PC. I use some espresso cups to lift the rack above the water, use whatever you have that will keep your rack level:


Make sure there's a tiny bit of space between individual stacks. Otherwise, they'll all stick together. If you don't have enough room to do that, but some tin foil between the stacks.

I do a 20min cycle with it. You can cycle it however long makes you feel comfortable. Let the PC cool down completely before moving it or anything. You want your agar to solidify before you take your dishes out, otherwise you'll spill over all your hard work.

Here come the dishes:


They will be a bit moist, but wrapped relatively tightly. There will be some holes in the saran wrap and that's a good thing. Just leave them on some shelf and they will dry out in a day or two. You can also go ahead and use them, but then I prefer to leave them unwrapped in a SAB for a day to let them dry out. I usually do that anyways.

Ok, so you grew some myc and some contams on those dishes and need to reuse them. I dump agar pucks into the toilet, but I'm not sure if that's recommended. My toilet sewage pipe basically goes straight down into the septic tank, so I can get away with lots of stuff.

Then I put them in a sink with some water and do some scrubbing:


I don't scrub too much. Just enough to remove the labels and any other visible debris. I put dishes into a sweaterbox sized tub, with edges pointing upwards. Once I'm done with all of them, I put some vinegar into the tub and cover all dishes with water:


This step is a must unless you get some super soft water out of your tap. It will keep your dishes nice and clear over time. Let it soak for a few hours.

Then put your dishes into a strainer, with edges pointing down:


Let it dry for a day and you have your dishes ready to be poured again.


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How I do glass dishes


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Offlinemushpunx
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26680259 - 05/19/20 03:59 AM (3 years, 8 months ago)

Nice! I didn't know cling wrap would hold up in a PC cycle, I would've thought to use foil.

If I wanted I could just sterilize the plates and pour them after they cool, yeah?


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Offlinepoisoned
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Re: Poisoned's glass petri no-pour tek. [Re: mushpunx]
    #26680279 - 05/19/20 04:18 AM (3 years, 8 months ago)

Yeah, for sure.

Cling foil does melt, but in a weird way, where it just pulls together.


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How I do glass dishes


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Offlinemushpunx
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26680443 - 05/19/20 06:12 AM (3 years, 8 months ago)

Makes sense. I might get a set of these, it would be nice to have them to put spores on, clone tissue, etc.. plates I'm gonna toss right away anyways.


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OfflineFrankRhizo
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Re: Poisoned's glass petri no-pour tek. [Re: mushpunx]
    #26680452 - 05/19/20 06:19 AM (3 years, 8 months ago)

I like it. Thanks.


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OfflineStinkBorg
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Re: Poisoned's glass petri no-pour tek. [Re: FrankRhizo]
    #26680488 - 05/19/20 06:55 AM (3 years, 8 months ago)

Very nice. I really appreciate the write up. You cleared up a lot of good questions I had surrounding the topic. I think you may have motivated me to go finally try working with glass dishes.

I also didnt know plastic cling wrap would hold up at all through the PC. Super cool!


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InvisibleThe Fresh PrintsS
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Re: Poisoned's glass petri no-pour tek. [Re: StinkBorg]
    #26680504 - 05/19/20 07:13 AM (3 years, 8 months ago)

Hell yes. I was just getting ready to buy a bunch more of the holy grail no-pour containers. I think I'll give this a try instead.


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Re: Poisoned's glass petri no-pour tek. [Re: The Fresh Prints] * 1
    #26680564 - 05/19/20 07:53 AM (3 years, 8 months ago)

Good to see a write-up on glass. I've just started experimenting with recycling my agar. Beginning with spore plates that didn't germinate. I just re-wrap them, re-pc them, reuse them. No problems, big time saver. One day soon will try with contaminated plates.


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Offlinepoisoned
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Re: Poisoned's glass petri no-pour tek. [Re: LadysKnight]
    #26680681 - 05/19/20 09:15 AM (3 years, 8 months ago)

It should work, but then you end up with some visible specks in your grow.


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How I do glass dishes


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InvisibleLadysKnight
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26680784 - 05/19/20 10:09 AM (3 years, 8 months ago)

Probably right, we shall see.
Did I mention I'm a cheap, lazy bastard?
Anyway, nice needed Tek, good job.


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Re: Poisoned's glass petri no-pour tek. [Re: LadysKnight] * 1
    #26683302 - 05/20/20 12:52 PM (3 years, 8 months ago)

Same here, I had no idea SW could survive PCing. The lifting of the rack is a genius idea, idk why I haven't thought of it before. I sterilize my dishes in a mycobag, which sucks for the most part, lol. I think I'll try your method next (or slightly modified).


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Offlinepoisoned
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Re: Poisoned's glass petri no-pour tek. [Re: Adas] * 1
    #26683568 - 05/20/20 03:11 PM (3 years, 8 months ago)

As said, SW doesn't survive it like you wrapped it. It will have some holes, it will melt together, but I find it even better that way.


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How I do glass dishes


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Offlineouuwee
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26683930 - 05/20/20 06:05 PM (3 years, 8 months ago)

Where do you get your glass petris


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Offlinepoisoned
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Re: Poisoned's glass petri no-pour tek. [Re: ouuwee]
    #26684683 - 05/21/20 01:06 AM (3 years, 8 months ago)

I'm located in central Europe and I get them for 0,75€/piece from a local lab store.


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How I do glass dishes


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OfflineAdas
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26684748 - 05/21/20 02:36 AM (3 years, 8 months ago)

I got mine from a German vendor but despite being pretty good, they weren't as specified. Not an honest vendor (they were smaller then they should have been).


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OfflineBobbit
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Re: Poisoned's glass petri no-pour tek. [Re: Adas]
    #26696098 - 05/26/20 02:09 AM (3 years, 7 months ago)

Nice writeup.

Will try raising the rack to allow more water for a slower cool. . .

Like the vinegar also. . . I've been drying properly till shiny, which is a lot of work. . .

For those looking to recycle plates, make sure you remove any visible colonies first. . .


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OfflineInthepit
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26701426 - 05/28/20 10:06 AM (3 years, 7 months ago)

Well I'll try it again. I think you encouraged me in another thread.
And even though I can't stand the smell of vinegar, I definitely want my new plates to stay pristine!
I totally dig my borosilicate glass and just use PP for fridge storage.


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Re: Poisoned's glass petri no-pour tek. [Re: Inthepit]
    #26701499 - 05/28/20 10:41 AM (3 years, 7 months ago)

Cool writeup nice work :super:


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Offlinepoisoned
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Re: Poisoned's glass petri no-pour tek. [Re: Pastywhyte]
    #26701766 - 05/28/20 12:42 PM (3 years, 7 months ago)

Thanks man! Means a lot to hear that from Pastywhyte himself!


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How I do glass dishes


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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26704804 - 05/29/20 02:23 PM (3 years, 7 months ago)

Really appreciated! I bought a 12 pack of pyrex when I first started and then decided to go with celltreat instead. I kind of hate wasting the plastic, though. I have a set of 40 Alien holy grails, too. They are great for general use. But I still felt bad about the box of unused pyrex. Now I am inspired to at least use these for clone masters for the best clarity.


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LAGM 2.024
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26759079 - 06/20/20 03:42 AM (3 years, 7 months ago)

Nice. Thanks. I'm gonna try this.
But what about condensation? Did you had any problems?


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Offlinepoisoned
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Re: Poisoned's glass petri no-pour tek. [Re: nosf3r4tu]
    #26760090 - 06/20/20 03:10 PM (3 years, 7 months ago)

There is usually some on the top plate, but I leave plates unwrapped in a SAB for at least a day after inoculation usually.


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Invisiblenosf3r4tu

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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26762098 - 06/21/20 02:11 AM (3 years, 7 months ago)

Ok. Thx.
I have them in the PC right now but I was having troubles getting the plates to stay on level so I decided to leave them as they are and pull them out while they're still hot and place them on a level surface.


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Offlinepoisoned
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Re: Poisoned's glass petri no-pour tek. [Re: nosf3r4tu]
    #26762402 - 06/21/20 07:24 AM (3 years, 7 months ago)

Good luck with that. I'd definitely screw that one up.


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How I do glass dishes


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InvisibleTheDoobskerS
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26776688 - 06/23/20 12:53 PM (3 years, 7 months ago)

Quote:

poisoned said:
There is usually some on the top plate, but I leave plates unwrapped in a SAB for at least a day after inoculation usually.




Question about this too...

There is so much condensation on the top glass after PC'ing that it's almost puddled. Logic tells me this is unavoidable with no poor. But, I also didn't notice them drying at all after leaving them in the SAB. Sure the very outer edges that were exposed to air dried, but 90% of the bottom plate is enclosed within the dish, so it doesn't dry.

I went ahead and inoculated anyway and am storing them upside down in the fridge.

Any suggestions? Are you saying that leaving them to dry actually reduces the condensation inside of the dish? or are you just referring to all outside surfaces when you say you leave them to dry?


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Invisiblenosf3r4tu

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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26778321 - 06/24/20 02:23 AM (3 years, 7 months ago)

I didn't screw it up to much... it's not perfect either.
When I pulled them out there was water inside the plastic wrap ( boilover) and I saw some air bubbles going in. Out of 25 plates that staid in SAB for 2 days I saw just 1 with contam and that could be a plate that I opened by mistake... inoculated about 10 plates today and now im waiting to see how it goes.
With a little bit of tweaking it can be done.


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Re: Poisoned's glass petri no-pour tek. [Re: nosf3r4tu]
    #26786575 - 06/27/20 07:32 AM (3 years, 6 months ago)

Thank you so much for this! :takingnotes: Actually buying some glass petris right now to make my agar work more environmentally friendly.

One (probably silly) question about saran wrap - does it come off easily after PC-ing and cooling down? Since it melts a little, isn't it hard to scrape it off after?


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OfflineInthepit
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Re: Poisoned's glass petri no-pour tek. [Re: ExactDepth]
    #26786702 - 06/27/20 08:39 AM (3 years, 6 months ago)

The cling wrap just peels off, kinda like a sunburn.
Watch out for the bottom plate falling out.
I had several plates open from my clumsiness.


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Re: Poisoned's glass petri no-pour tek. [Re: Inthepit]
    #26823111 - 07/14/20 01:37 PM (3 years, 6 months ago)

I've just tried it today and HOLY SHIT it worked smoothly! Thanks bro for inventing this. Wonderful stuff, this is how I'm doing it from now on.


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OfflineDrBones666
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Re: Poisoned's glass petri no-pour tek. [Re: mushpunx]
    #27097677 - 12/19/20 12:27 PM (3 years, 1 month ago)

This is exactly the post I've been wanting.
Thanks to IdiotCircusBoy for pointing it out to me.


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OfflineRegis
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #27198482 - 02/11/21 12:56 AM (2 years, 11 months ago)

Great tek!
I've been using it nearly the same way. Only difference, i wrapped the sides of the dishes individually with a cut off roll of saran wrap and put a stack of them in aluminium foil.
Will try it your way for the next batch.


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OfflineMoopers
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Re: Poisoned's glass petri no-pour tek. [Re: Regis]
    #28554471 - 11/23/23 09:40 PM (2 months, 3 days ago)

Trying this tonight! Hope I wrapped them right/enough. Is that important for success that the plastic doesn't come open during PCing?


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Re: Poisoned's glass petri no-pour tek. [Re: Moopers]
    #28554601 - 11/24/23 03:03 AM (2 months, 2 days ago)

Nice,
I ll give it a try tonight.
Thanks for the write up


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OfflineMoopers
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Re: Poisoned's glass petri no-pour tek. [Re: Traveled]
    #28554959 - 11/24/23 12:19 PM (2 months, 2 days ago)

I tried it last night with 24 plates. I was hoping this would solve my condensation issues, but 22/24 plates had bad condensation! I could use help figuring out what's going wrong. Recap of method is below.

  • Recipe was 10g agar, 8g LME, 500ml distilled water, whisked well and left to sit for an hour, then simmered on the stove until very lightly bubbling, then stirred and cooked for a few mins.
  • Of the 24 petris, 6 are PP5, the rest are glass. Glass ones are 20 pyrex 10x15s and 4 cheap 10x20 ones from Amazon.
  • Plates were poured, then left to cool (30 min), then lidded, stacked (did a stack of 2 and 3 plates, the rest 4 plates) and wrapped in plastic wrap by placing the stack in the center of a strip of plastic wrap and folding up to the top, making sure everything looked covered.
  • PC was brought to temp, vented for 10 minutes before the weight, then PC'd for 15 min at 15 psi, left to cool overnight.
  • 22/24 plates are soaked and have puddling condensation. Plastic wrap had little melted holes but didn't unravel.


There's much more condensation than when I wrap in foil doing the hazyhorse "I love glass petris" method. I always get condensation with foil, but it's not this bad.

Any thoughts on how to troubleshoot no pour condensation, or just general tips on avoiding it, would be appreciated. Am I not cooking the agar long enough? Possible I just did a bad job wrapping the dishes?


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OfflineKinoko314
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Re: Poisoned's glass petri no-pour tek. [Re: Moopers]
    #28554998 - 11/24/23 12:52 PM (2 months, 2 days ago)

Condensation forms as it cools, and I think it's worse if it cools fast.  Did you let your plates cool off slowly in the PC?

I've accepted condensation as a fact of life when doing no-pours.  I don't have glass plates, but I think it's pretty similar to all no-pour plates.  Normally I let the condensation clear in a small tub with a ton of desiccant.  If you have a FH though, just put them in front of that for a while.

The holes that form from the partial melting of the cling wrap is supposed to allow for that evaporation.  I want to try this with my PP plates.  It would save me the trouble of wrapping stacks after the condensation dries.


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OfflineMoopers
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Re: Poisoned's glass petri no-pour tek. [Re: Kinoko314]
    #28555241 - 11/24/23 04:48 PM (2 months, 2 days ago)

Quote:

Kinoko314 said:Condensation forms as it cools, and I think it's worse if it cools fast.  Did you let your plates cool off slowly in the PC? ... The holes that form from the partial melting of the cling wrap is supposed to allow for that evaporation.



I turned off the heat and let it cool overnight. I have them in an SAB unwrapped. I am seriously considering a flow hood, though.


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Re: Poisoned's glass petri no-pour tek. [Re: Moopers]
    #28555257 - 11/24/23 05:05 PM (2 months, 2 days ago)

My best go so far was to pc the poured plates in stacks of five, double wrapped in foil. Then take straight from the pc to the sab, put them inside, unwrap them, then let them dry out for a few days before using/wrapping them. Practically no condensation, no contams, and no shit stuck to the plates. Oh, and they didn't stick together when I tried to do transfers. I used plates straight out the pc first and they created a vacuum and were so hard to take the lids off and separate plates. Got lots of contam that way.
Plastic plates are so much easier but i hating polluting our mother earth.


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OfflineHappinessStan
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Re: Poisoned's glass petri no-pour tek. [Re: Moopers]
    #28555259 - 11/24/23 05:05 PM (2 months, 2 days ago)

Quote:

Moopers said:
Quote:

Kinoko314 said:Condensation forms as it cools, and I think it's worse if it cools fast.  Did you let your plates cool off slowly in the PC? ... The holes that form from the partial melting of the cling wrap is supposed to allow for that evaporation.



I turned off the heat and let it cool overnight. I have them in an SAB unwrapped. I am seriously considering a flow hood, though.



Sabs work fine if you work on your sterile technique. Who doesn't want a flowhood though?


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OfflineMoopers
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Re: Poisoned's glass petri no-pour tek. [Re: HappinessStan]
    #28556064 - 11/25/23 01:59 PM (2 months, 1 day ago)

Quote:

HappinessStan said:
Quote:

Moopers said:
Quote:

Kinoko314 said:Condensation forms as it cools, and I think it's worse if it cools fast.  Did you let your plates cool off slowly in the PC? ... The holes that form from the partial melting of the cling wrap is supposed to allow for that evaporation.



I turned off the heat and let it cool overnight. I have them in an SAB unwrapped. I am seriously considering a flow hood, though.



Sabs work fine if you work on your sterile technique. Who doesn't want a flowhood though?




I ordered a flow hood out of frustration. It's been discouraging trying to figure out what's wrong.

I'm having issues germinating spores on my plates, tho they grow transfers just fine. There are no obvious contams, but spores from prints taken in 2021 are not germinating at all on the plates I've made. I've tried softer agar, but the agar wasn't that hard to begin with. These are plates I left to dry in the SAB for a few days, but there was still a little condensation on the lids. Could this condensation be the issue?

I switched to distilled water this last batch in case it's a tap water issue. A couple days ago I inoculated plates using a fresh print from this year in case the 5 prints I tried were all too old or didn't like how they were stored. Fingers crossed.

On the way is the flow hood (yay), an agar/media bottle, and some agar mix and prepoured agar plates to continue the troubleshooting.


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Re: Poisoned's glass petri no-pour tek. [Re: Moopers]
    #28556078 - 11/25/23 02:11 PM (2 months, 1 day ago)

Quote:

Moopers said:
Quote:

HappinessStan said:
Quote:

Moopers said:
Quote:

Kinoko314 said:Condensation forms as it cools, and I think it's worse if it cools fast.  Did you let your plates cool off slowly in the PC? ... The holes that form from the partial melting of the cling wrap is supposed to allow for that evaporation.



I turned off the heat and let it cool overnight. I have them in an SAB unwrapped. I am seriously considering a flow hood, though.



Sabs work fine if you work on your sterile technique. Who doesn't want a flowhood though?




I ordered a flow hood out of frustration. It's been discouraging trying to figure out what's wrong.

I'm having issues germinating spores on my plates, tho they grow transfers just fine. There are no obvious contams, but spores from prints taken in 2021 are not germinating at all on the plates I've made. I've tried softer agar, but the agar wasn't that hard to begin with. These are plates I left to dry in the SAB for a few days, but there was still a little condensation on the lids. Could this condensation be the issue?

I switched to distilled water this last batch in case it's a tap water issue. A couple days ago I inoculated plates using a fresh print from this year in case the 5 prints I tried were all too old or didn't like how they were stored. Fingers crossed.

On the way is the flow hood (yay), an agar/media bottle, and some agar mix and prepoured agar plates to continue the troubleshooting.





It's quite possible those spores are simply dead and you may have done nothing wrong.


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