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Invisiblenosf3r4tu

Registered: 03/26/19
Posts: 775
Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26759079 - 06/20/20 03:42 AM (3 years, 7 months ago)

Nice. Thanks. I'm gonna try this.
But what about condensation? Did you had any problems?


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Offlinepoisoned
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Registered: 04/17/13
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Re: Poisoned's glass petri no-pour tek. [Re: nosf3r4tu]
    #26760090 - 06/20/20 03:10 PM (3 years, 7 months ago)

There is usually some on the top plate, but I leave plates unwrapped in a SAB for at least a day after inoculation usually.


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How I do glass dishes


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Invisiblenosf3r4tu

Registered: 03/26/19
Posts: 775
Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26762098 - 06/21/20 02:11 AM (3 years, 7 months ago)

Ok. Thx.
I have them in the PC right now but I was having troubles getting the plates to stay on level so I decided to leave them as they are and pull them out while they're still hot and place them on a level surface.


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Offlinepoisoned
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Registered: 04/17/13
Posts: 1,738
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Re: Poisoned's glass petri no-pour tek. [Re: nosf3r4tu]
    #26762402 - 06/21/20 07:24 AM (3 years, 7 months ago)

Good luck with that. I'd definitely screw that one up.


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How I do glass dishes


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InvisibleTheDoobskerS
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Registered: 05/22/20
Posts: 799
Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26776688 - 06/23/20 12:53 PM (3 years, 7 months ago)

Quote:

poisoned said:
There is usually some on the top plate, but I leave plates unwrapped in a SAB for at least a day after inoculation usually.




Question about this too...

There is so much condensation on the top glass after PC'ing that it's almost puddled. Logic tells me this is unavoidable with no poor. But, I also didn't notice them drying at all after leaving them in the SAB. Sure the very outer edges that were exposed to air dried, but 90% of the bottom plate is enclosed within the dish, so it doesn't dry.

I went ahead and inoculated anyway and am storing them upside down in the fridge.

Any suggestions? Are you saying that leaving them to dry actually reduces the condensation inside of the dish? or are you just referring to all outside surfaces when you say you leave them to dry?


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Invisiblenosf3r4tu

Registered: 03/26/19
Posts: 775
Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #26778321 - 06/24/20 02:23 AM (3 years, 7 months ago)

I didn't screw it up to much... it's not perfect either.
When I pulled them out there was water inside the plastic wrap ( boilover) and I saw some air bubbles going in. Out of 25 plates that staid in SAB for 2 days I saw just 1 with contam and that could be a plate that I opened by mistake... inoculated about 10 plates today and now im waiting to see how it goes.
With a little bit of tweaking it can be done.


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OfflineExactDepth

Registered: 03/31/20
Posts: 1
Loc: London
Last seen: 3 years, 5 months
Re: Poisoned's glass petri no-pour tek. [Re: nosf3r4tu]
    #26786575 - 06/27/20 07:32 AM (3 years, 6 months ago)

Thank you so much for this! :takingnotes: Actually buying some glass petris right now to make my agar work more environmentally friendly.

One (probably silly) question about saran wrap - does it come off easily after PC-ing and cooling down? Since it melts a little, isn't it hard to scrape it off after?


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OfflineInthepit
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Registered: 08/20/19
Posts: 1,742
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Re: Poisoned's glass petri no-pour tek. [Re: ExactDepth]
    #26786702 - 06/27/20 08:39 AM (3 years, 6 months ago)

The cling wrap just peels off, kinda like a sunburn.
Watch out for the bottom plate falling out.
I had several plates open from my clumsiness.


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OfflineAdas
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Re: Poisoned's glass petri no-pour tek. [Re: Inthepit]
    #26823111 - 07/14/20 01:37 PM (3 years, 6 months ago)

I've just tried it today and HOLY SHIT it worked smoothly! Thanks bro for inventing this. Wonderful stuff, this is how I'm doing it from now on.


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OfflineDrBones666
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Registered: 10/27/20
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Re: Poisoned's glass petri no-pour tek. [Re: mushpunx]
    #27097677 - 12/19/20 12:27 PM (3 years, 1 month ago)

This is exactly the post I've been wanting.
Thanks to IdiotCircusBoy for pointing it out to me.


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OfflineRegis
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Registered: 06/07/20
Posts: 273
Loc: Germany
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
    #27198482 - 02/11/21 12:56 AM (2 years, 11 months ago)

Great tek!
I've been using it nearly the same way. Only difference, i wrapped the sides of the dishes individually with a cut off roll of saran wrap and put a stack of them in aluminium foil.
Will try it your way for the next batch.


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OfflineMoopers
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Registered: 05/08/20
Posts: 525
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Re: Poisoned's glass petri no-pour tek. [Re: Regis]
    #28554471 - 11/23/23 09:40 PM (2 months, 3 days ago)

Trying this tonight! Hope I wrapped them right/enough. Is that important for success that the plastic doesn't come open during PCing?


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OfflineTraveled
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Registered: 08/23/23
Posts: 108
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Re: Poisoned's glass petri no-pour tek. [Re: Moopers]
    #28554601 - 11/24/23 03:03 AM (2 months, 2 days ago)

Nice,
I ll give it a try tonight.
Thanks for the write up


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OfflineMoopers
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Registered: 05/08/20
Posts: 525
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Re: Poisoned's glass petri no-pour tek. [Re: Traveled]
    #28554959 - 11/24/23 12:19 PM (2 months, 2 days ago)

I tried it last night with 24 plates. I was hoping this would solve my condensation issues, but 22/24 plates had bad condensation! I could use help figuring out what's going wrong. Recap of method is below.

  • Recipe was 10g agar, 8g LME, 500ml distilled water, whisked well and left to sit for an hour, then simmered on the stove until very lightly bubbling, then stirred and cooked for a few mins.
  • Of the 24 petris, 6 are PP5, the rest are glass. Glass ones are 20 pyrex 10x15s and 4 cheap 10x20 ones from Amazon.
  • Plates were poured, then left to cool (30 min), then lidded, stacked (did a stack of 2 and 3 plates, the rest 4 plates) and wrapped in plastic wrap by placing the stack in the center of a strip of plastic wrap and folding up to the top, making sure everything looked covered.
  • PC was brought to temp, vented for 10 minutes before the weight, then PC'd for 15 min at 15 psi, left to cool overnight.
  • 22/24 plates are soaked and have puddling condensation. Plastic wrap had little melted holes but didn't unravel.


There's much more condensation than when I wrap in foil doing the hazyhorse "I love glass petris" method. I always get condensation with foil, but it's not this bad.

Any thoughts on how to troubleshoot no pour condensation, or just general tips on avoiding it, would be appreciated. Am I not cooking the agar long enough? Possible I just did a bad job wrapping the dishes?


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OfflineKinoko314
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Registered: 12/16/22
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Re: Poisoned's glass petri no-pour tek. [Re: Moopers]
    #28554998 - 11/24/23 12:52 PM (2 months, 2 days ago)

Condensation forms as it cools, and I think it's worse if it cools fast.  Did you let your plates cool off slowly in the PC?

I've accepted condensation as a fact of life when doing no-pours.  I don't have glass plates, but I think it's pretty similar to all no-pour plates.  Normally I let the condensation clear in a small tub with a ton of desiccant.  If you have a FH though, just put them in front of that for a while.

The holes that form from the partial melting of the cling wrap is supposed to allow for that evaporation.  I want to try this with my PP plates.  It would save me the trouble of wrapping stacks after the condensation dries.


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OfflineMoopers
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Registered: 05/08/20
Posts: 525
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Re: Poisoned's glass petri no-pour tek. [Re: Kinoko314]
    #28555241 - 11/24/23 04:48 PM (2 months, 2 days ago)

Quote:

Kinoko314 said:Condensation forms as it cools, and I think it's worse if it cools fast.  Did you let your plates cool off slowly in the PC? ... The holes that form from the partial melting of the cling wrap is supposed to allow for that evaporation.



I turned off the heat and let it cool overnight. I have them in an SAB unwrapped. I am seriously considering a flow hood, though.


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OfflineHappinessStan
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Registered: 10/10/12
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Loc: Worcester, UK
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Re: Poisoned's glass petri no-pour tek. [Re: Moopers]
    #28555257 - 11/24/23 05:05 PM (2 months, 2 days ago)

My best go so far was to pc the poured plates in stacks of five, double wrapped in foil. Then take straight from the pc to the sab, put them inside, unwrap them, then let them dry out for a few days before using/wrapping them. Practically no condensation, no contams, and no shit stuck to the plates. Oh, and they didn't stick together when I tried to do transfers. I used plates straight out the pc first and they created a vacuum and were so hard to take the lids off and separate plates. Got lots of contam that way.
Plastic plates are so much easier but i hating polluting our mother earth.


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OfflineHappinessStan
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Re: Poisoned's glass petri no-pour tek. [Re: Moopers]
    #28555259 - 11/24/23 05:05 PM (2 months, 2 days ago)

Quote:

Moopers said:
Quote:

Kinoko314 said:Condensation forms as it cools, and I think it's worse if it cools fast.  Did you let your plates cool off slowly in the PC? ... The holes that form from the partial melting of the cling wrap is supposed to allow for that evaporation.



I turned off the heat and let it cool overnight. I have them in an SAB unwrapped. I am seriously considering a flow hood, though.



Sabs work fine if you work on your sterile technique. Who doesn't want a flowhood though?


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OfflineMoopers
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Registered: 05/08/20
Posts: 525
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Re: Poisoned's glass petri no-pour tek. [Re: HappinessStan]
    #28556064 - 11/25/23 01:59 PM (2 months, 1 day ago)

Quote:

HappinessStan said:
Quote:

Moopers said:
Quote:

Kinoko314 said:Condensation forms as it cools, and I think it's worse if it cools fast.  Did you let your plates cool off slowly in the PC? ... The holes that form from the partial melting of the cling wrap is supposed to allow for that evaporation.



I turned off the heat and let it cool overnight. I have them in an SAB unwrapped. I am seriously considering a flow hood, though.



Sabs work fine if you work on your sterile technique. Who doesn't want a flowhood though?




I ordered a flow hood out of frustration. It's been discouraging trying to figure out what's wrong.

I'm having issues germinating spores on my plates, tho they grow transfers just fine. There are no obvious contams, but spores from prints taken in 2021 are not germinating at all on the plates I've made. I've tried softer agar, but the agar wasn't that hard to begin with. These are plates I left to dry in the SAB for a few days, but there was still a little condensation on the lids. Could this condensation be the issue?

I switched to distilled water this last batch in case it's a tap water issue. A couple days ago I inoculated plates using a fresh print from this year in case the 5 prints I tried were all too old or didn't like how they were stored. Fingers crossed.

On the way is the flow hood (yay), an agar/media bottle, and some agar mix and prepoured agar plates to continue the troubleshooting.


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OfflineRockinRobot
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Re: Poisoned's glass petri no-pour tek. [Re: Moopers]
    #28556078 - 11/25/23 02:11 PM (2 months, 1 day ago)

Quote:

Moopers said:
Quote:

HappinessStan said:
Quote:

Moopers said:
Quote:

Kinoko314 said:Condensation forms as it cools, and I think it's worse if it cools fast.  Did you let your plates cool off slowly in the PC? ... The holes that form from the partial melting of the cling wrap is supposed to allow for that evaporation.



I turned off the heat and let it cool overnight. I have them in an SAB unwrapped. I am seriously considering a flow hood, though.



Sabs work fine if you work on your sterile technique. Who doesn't want a flowhood though?




I ordered a flow hood out of frustration. It's been discouraging trying to figure out what's wrong.

I'm having issues germinating spores on my plates, tho they grow transfers just fine. There are no obvious contams, but spores from prints taken in 2021 are not germinating at all on the plates I've made. I've tried softer agar, but the agar wasn't that hard to begin with. These are plates I left to dry in the SAB for a few days, but there was still a little condensation on the lids. Could this condensation be the issue?

I switched to distilled water this last batch in case it's a tap water issue. A couple days ago I inoculated plates using a fresh print from this year in case the 5 prints I tried were all too old or didn't like how they were stored. Fingers crossed.

On the way is the flow hood (yay), an agar/media bottle, and some agar mix and prepoured agar plates to continue the troubleshooting.





It's quite possible those spores are simply dead and you may have done nothing wrong.


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