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kgq
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Slow Mycelium growth in my spawn jars
#26680131 - 05/19/20 01:15 AM (3 years, 8 months ago) |
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Hi,
It's my first time ever growing mushrooms and I ran into a bit of a problem. My jars are progressing super slowly. My first jar started to have the first signs of growth 7 days after inoculation by Spore Syringe and has been progressing for 5 days since then, but it's still only a small patch of mycelium.
I read here it can be due to too little air exchange, but I used micropore tape over a hole I drilled into the lid (12mm/0.4 inches in diameter). Maybe it's too small? I hope you can tell me.
Edited by kgq (05/19/20 01:26 AM)
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ModularMind
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Re: Slow Mycelium growth in my spawn jars [Re: kgq]
#26680157 - 05/19/20 02:11 AM (3 years, 8 months ago) |
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That hole is plenty big. How did you prepare your grains?
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kgq
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Re: Slow Mycelium growth in my spawn jars [Re: ModularMind]
#26680179 - 05/19/20 02:25 AM (3 years, 8 months ago) |
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It's Rye grain. I washed it, cooked it for 10 minutes, let it dry until they didn't leave a spot on a paper towel anymore, then loaded the jars, covered the lids with tinfoil and pressure cooked for like 110min. I then let it cool for I think 2 hours until they weren't warm to the touch anymore. Then I inoculated through the port on the top.
It was a tek from this site. I can't remember which one though. I modified it to my needs a bit.
After opening this thread I found out that hole might not only be plenty big, but too big. I also noticed that the grain is looking a bit dry, could that be the problem? If yes, I have a bunch of new jars and can try again anytime, but I really want to know what I should do different this time.
Also, can I harvest the bit of mycelium to do a G2G or is it too risky to get it out without causing contamination? I'm also in the process of colonizing agar dishes, but I think they'll take a bit more time before I can use these to do a transfer.
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ModularMind
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Re: Slow Mycelium growth in my spawn jars [Re: kgq]
#26680187 - 05/19/20 02:30 AM (3 years, 8 months ago) |
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Did you soak your rye or just boil it for ten minutes? It may not have absorbed sufficient moisture.
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kgq
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Re: Slow Mycelium growth in my spawn jars [Re: ModularMind]
#26680192 - 05/19/20 02:35 AM (3 years, 8 months ago) |
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Oh right I forgot. I let it soak for 24hours before cooking..
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ModularMind
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Re: Slow Mycelium growth in my spawn jars [Re: kgq]
#26680198 - 05/19/20 02:41 AM (3 years, 8 months ago) |
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Honestly, it’s not that long since MS inoculation. What’s there will pick up steam soon. Once it is ~30% you can shake. Keep us posted.
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kgq
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Re: Slow Mycelium growth in my spawn jars [Re: ModularMind]
#26680205 - 05/19/20 02:49 AM (3 years, 8 months ago) |
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I'm just a bit impatient, because I see all these "fully colonized after 10 days jars" and mine didn't even accelerate after showing first signs of growth.
I'm at my last 4cc of Spore Solution and it's hard to come by where I live, so I don't want to make the same mistakes again.
Should I try with a smaller drill hole, with the same grain preparation tek, while letting this jar sit?
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ModularMind
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Re: Slow Mycelium growth in my spawn jars [Re: kgq]
#26680209 - 05/19/20 02:55 AM (3 years, 8 months ago) |
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Any ability to prepare agar plates? I would certainly suggest putting a drop on a few plates.
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kgq
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Re: Slow Mycelium growth in my spawn jars [Re: ModularMind]
#26680217 - 05/19/20 03:06 AM (3 years, 8 months ago) |
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Yep, got 4 plates with different amounts of spore solution prepared. Only one is showing any growth after 5 days.. the one I put like 1cc by accident on. The others I inoculated with the recommended amount (1-3 drops) look as clean as when I got them. (I bought pre-prepared agar).
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ModularMind
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Re: Slow Mycelium growth in my spawn jars [Re: kgq]
#26680221 - 05/19/20 03:11 AM (3 years, 8 months ago) |
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At least you’re diversified. 5 days is still early.
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kgq
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Re: Slow Mycelium growth in my spawn jars [Re: ModularMind]
#26680226 - 05/19/20 03:18 AM (3 years, 8 months ago) |
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I always try to do little experiments while still following the teks. The jar on the image was inoculated with 1.5cc and showed signs of growth 3 days earlier than the same type of jar inoculated with 1cc.
Edited by kgq (05/19/20 03:18 AM)
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ModularMind
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Re: Slow Mycelium growth in my spawn jars [Re: kgq]
#26680232 - 05/19/20 03:24 AM (3 years, 8 months ago) |
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That’s pretty good restraint for a MS syringe.
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mushpunx
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Re: Slow Mycelium growth in my spawn jars [Re: kgq]
#26680265 - 05/19/20 04:04 AM (3 years, 8 months ago) |
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Quote:
kgq said: Yep, got 4 plates with different amounts of spore solution prepared. Only one is showing any growth after 5 days.. the one I put like 1cc by accident on. The others I inoculated with the recommended amount (1-3 drops) look as clean as when I got them. (I bought pre-prepared agar).
Less is better, just use one drop. Don't forget to swipe across the plate with a sterilized loop too, it makes it easier to identify and transfer clean myc away from bacteria or mold colonies.
Spore to grain can be slow. Once it colonized a spot the size of a small fist break it up and give it a shake- should finish in a few days
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Re: Slow Mycelium growth in my spawn jars [Re: mushpunx]
#26680505 - 05/19/20 07:13 AM (3 years, 8 months ago) |
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The hole size is fine.
What you’ve got is pretty normal for five days since germination.
It’s random some are faster some are slower, yours is a little on the slow side but still within normal imo.
Also the myc goes faster the more there is. It’ll cover as much in the last three days as it did in the first 8 a lot of times.
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Re: Slow Mycelium growth in my spawn jars [Re: A.k.a]
#26680530 - 05/19/20 07:36 AM (3 years, 8 months ago) |
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A lot of the 10 day colonization you see is from everything except spores to grain. Agar wedges, LC/LI, g2g. With spores to grain you have to wait for the spores to actually germinate first, whereas with those other methods you’re putting already live mycelium in there and it takes a second to recover and then it’s off doing its thing
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kgq
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Re: Slow Mycelium growth in my spawn jars [Re: mushpunx]
#26680593 - 05/19/20 08:16 AM (3 years, 8 months ago) |
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Quote:
mushpunx said: Less is better, just use one drop. Don't forget to swipe across the plate with a sterilized loop too, it makes it easier to identify and transfer clean myc away from bacteria or mold colonies.
whoops, didn't even know about streaking and sterilized loops before lol. I just put a few drops on the same spot and closed them up for good. Will they be fine? One already has a little mycelium culture, but not really something I can harvest.
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Re: Slow Mycelium growth in my spawn jars [Re: kgq]
#26680600 - 05/19/20 08:19 AM (3 years, 8 months ago) |
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It’ll be fine, it’ll probably just be super fuzzy from all the spores growing on top of each other.
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kgq
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Re: Slow Mycelium growth in my spawn jars [Re: A.k.a]
#26680619 - 05/19/20 08:34 AM (3 years, 8 months ago) |
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My goal from the beginning was to first multiply the spores I got in the syringe, because the amount I bought isn't really viable to do experiments with (10cc). What would you recommend I do with last 4cc? LC, more Agar Dishes or another Grain Spawn Jar?
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Re: Slow Mycelium growth in my spawn jars [Re: kgq] 1
#26680625 - 05/19/20 08:40 AM (3 years, 8 months ago) |
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I wouldn’t do anything yet, keep it in the fridge in case the stuff going now doesn’t work out.
If you’ve got plates going already just work with those.
You have myc so you should be able to grow it and then have all the spores you want.
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mushpunx
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Re: Slow Mycelium growth in my spawn jars [Re: kgq]
#26680649 - 05/19/20 09:03 AM (3 years, 8 months ago) |
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Quote:
kgq said: My goal from the beginning was to first multiply the spores I got in the syringe, because the amount I bought isn't really viable to do experiments with (10cc). What would you recommend I do with last 4cc? LC, more Agar Dishes or another Grain Spawn Jar?
Well LC should *never* be started from spore solution. Grains either really. They both can work but there is a high chance of contamination. Spore prints are made from prints that have been grown in open air - we reduce the contams by printing mushrooms that were grown indoors onto sterile foil - but the prints will never be sterile and that means the syringes won't be either. They are usually clean enough for BRF cakes, they are pretty forgiving. In an LC that bacteria or mold will expand rapidly.
Agar gives us the chance to transfer clean myc away from bacteria or mold to a new plates until we have a clean culture to inoculate grains or LC with.
I'd just hold on to that last 4cc. It will last for a long time if you are using agar. You will be able to make more syringes from caps but if you are working with agar no real need - the print is more valuable to you.
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