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Noisecontrol
Test Tube Baby



Registered: 05/07/15
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LC from D5%
#26679560 - 05/18/20 06:24 PM (3 years, 8 months ago) |
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Wondering if anyone with access to medical supplies have tried sterile D5% bags as mediums for their LC and to what level of success.
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Solipsis
m̶a̶d̶ disappointed scientist


Registered: 12/28/09
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No but could you describe what you think is the advantage to a more practical container? Yes you may want to especially modify it but at least you could magnetically stir a jar properly 
Hypotheticals analysis could give some insight as to whether it's worthwhile to even try.
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Noisecontrol
Test Tube Baby



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The advantage is availability. I have said bags on hand and would save from having to mix the solution and sterilize it. It's essentially premixed pre-sterilized solution
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Solipsis
m̶a̶d̶ disappointed scientist


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OK, well it dextrose is the only thing it contains that can be limiting because fungi also need micronutrition and things like nitrogen to grow.
Dextrose is only a carbon source. The deficiency of the rest will be the limiting factor.
Then it depends what you inoculate with and how much micronutrition you pass along with the inoculant.
So it should generally be expected to work a little but not well. I like needle biopsy which does not inoculate with much matter at all, its just a little myc, no real reserves. It would work a lot better if you inoculate with a decent quantity of other LC that comes with micronutrition but i guess the whole point is you wanna make that LC.
Still, means you can expand differently made LC into D5W
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SannyTIES



Registered: 07/02/19
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Loc: The big easy
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https://www.shroomery.org/forums/showflat.php/Number/25100192/fpart/all/vc/1
This is a common, easy to locate write up on more than one post.
A google search found this link above in our forum.
Edited by SannyTIES (05/21/20 02:34 AM)
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RoscoeReturns
Crotchety chode man


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I’ve used 500ml D5 bags to make liquid culture with quite a bit of success. They are really quick and easy to use, and at least in the case of the edibles I was cultivating, I ended up with more clean LC than I could use in reasonable amount of time. I think I ended up drawing up some syringes to give to friends.
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Solipsis
m̶a̶d̶ disappointed scientist


Registered: 12/28/09
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Nice, plz add info on what you inoculated with. As said it can make a lot of difference
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Noisecontrol
Test Tube Baby



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If/when I try this I will post results. Thanks for the thinktank, friends
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Solipsis
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RoscoeReturns
Crotchety chode man


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Quote:
Solipsis said: Nice, plz add info on what you inoculated with. As said it can make a lot of difference
I shot LC of shiitake, blue oyster, lion’s mane, and rieshi into 500ml D5 bags. All grew pretty well hanging in my closet. I would shake the bag, and draw out to use to inoculate grain jars. Having sealed sterile bags makes clean growing easier. It’s not necessary, but I had access to bags for free. I don’t think they would be worth the cost if I were buying them.
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Noisecontrol
Test Tube Baby



Registered: 05/07/15
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I agree and am in the same position of having the opportunity and convenience of them.
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 Happy to help, happier to learn Feel free to slide in my DM To the man in a wheelchair who stole my camouflage jacket... ...you can hide but you can't run... Trade List
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