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OfflineJbrady5555
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First oat jar innoc moving slowly
    #26668939 - 05/13/20 05:02 PM (3 years, 8 months ago)

So I inoculated 10 whole oat quart jars 5 days ago with agar wedges that were on their second transfer. This is my first time prepping jars and it seems to be moving slower than what I’ve seen from others here. I’ve been trying to keep the temp in the high 60s but a few nights did drop into the 50’s. Included is a pic, just trying to get critique and info. Learning as I go.



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Offlineouuwee
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Re: First oat jar innoc moving slowly [Re: Jbrady5555]
    #26669035 - 05/13/20 05:50 PM (3 years, 8 months ago)

Temperatures are too low which is why the growth is slow. Ideally you want a constant ~70s for optimal growth


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OfflineA.k.aM
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Re: First oat jar innoc moving slowly [Re: ouuwee]
    #26669171 - 05/13/20 06:50 PM (3 years, 8 months ago)

Yeah those temps are fine for tubs but ime spawn jars will pretty much stop around 60.


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Offlineshevanel
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Re: First oat jar innoc moving slowly [Re: A.k.a] * 1
    #26669373 - 05/13/20 08:12 PM (3 years, 8 months ago)

Also, aside from those Alaskan temps agar as an inoculate is just slower than you expect. It takes about a good 24-48 hours before the myc recovers then a few more days[ at least ]to jump to the grains. Then a few more to get going ... Then a few more to finish up.

Avg for me is about 12-14 days give or take. That's why I don't usually use agar as an inoculate unless I'm trying to end a grow and use it up. Mostly use it for LC. Once the LC is ready it'll colonize a jar in less than a week. Usually 5 days.

1/8th of a petri will make 500-750ml of LC within a week and that will innoc. Many many jars saving time in the long run. As opposed to 1 plate and wait.


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OfflineJbrady5555
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Re: First oat jar innoc moving slowly [Re: shevanel]
    #26678797 - 05/18/20 11:26 AM (3 years, 8 months ago)

Quote:

shevanel said:
Also, aside from those Alaskan temps agar as an inoculate is just slower than you expect. It takes about a good 24-48 hours before the myc recovers then a few more days[ at least ]to jump to the grains. Then a few more to get going ... Then a few more to finish up.

Avg for me is about 12-14 days give or take. That's why I don't usually use agar as an inoculate unless I'm trying to end a grow and use it up. Mostly use it for LC. Once the LC is ready it'll colonize a jar in less than a week. Usually 5 days.

1/8th of a petri will make 500-750ml of LC within a week and that will innoc. Many many jars saving time in the long run. As opposed to 1 plate and wait.




Thanks all. I guess I need to learn to make a liquid culture.  My jars are still super slow but they are growing a little.


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OfflineJbrady5555
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Re: First oat jar innoc moving slowly [Re: Jbrady5555]
    #26678800 - 05/18/20 11:29 AM (3 years, 8 months ago)



So yea still moving slowly. Inoculated on the 8th, first pic was on the 13th and second pic was yesterday. 10 days in and moving like molasses.


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Offlinemarvins
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Re: First oat jar innoc moving slowly [Re: Jbrady5555]
    #26678921 - 05/18/20 12:41 PM (3 years, 8 months ago)

Im a total noob but comparing with other people jars I see too much space between grains in you pictures. But im a noob so maybe its good.


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Offlinepoisoned
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Re: First oat jar innoc moving slowly [Re: marvins]
    #26678933 - 05/18/20 12:45 PM (3 years, 8 months ago)

Nah, having some space between grains is good. I'd wait another day or two and do the shake.


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InvisibleRoger Clemency
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Re: First oat jar innoc moving slowly [Re: marvins]
    #26678946 - 05/18/20 12:50 PM (3 years, 8 months ago)

You might also want to check out the Blenderless LI tek. It has worked well for me and I’ve already spawned jars I inoculated with LI about two weeks after some jars I hit with wedges that still aren’t done.


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