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NightPuma1
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Storing agar petri dishes in the refrigerator question 1
#26677392 - 05/17/20 06:10 PM (3 years, 8 months ago) |
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Hey guys I made some agar Petri dishes and I wrapped them in parafilm and put them in the refrigerator and then when I went to take them out and use them all the condensation seemed to have fallen onto the agar. Anyone have any experience with this?
Do you guys like take your agar petri dishes out of the refrigerator a certain length of time before you plan on using them?
Thank you!
Edited by NightPuma1 (05/17/20 06:30 PM)
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WeavieWonder
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Re: Storing agar petri dishes in the refrigerator question [Re: NightPuma1] 1
#26677482 - 05/17/20 06:54 PM (3 years, 8 months ago) |
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No need to refrigerate petris. Take em out and let them clear up before using. They will be fine. No need to wrap them in parafilm either until you inoculate them.
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Nj.Diesel
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Re: Storing agar petri dishes in the refrigerator question [Re: WeavieWonder] 1
#26677700 - 05/17/20 08:39 PM (3 years, 8 months ago) |
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Quote:
WeavieWonder said: No need to refrigerate petris. Take em out and let them clear up before using. They will be fine. No need to wrap them in parafilm either until you inoculate them.
From BOD’s agar tek he said that if you pour plates and want to save them for later you should: pour> cool> wrap in the plastic sleeve they come with> refrigerate. Is that immaculate? (Might be outdated).
Asking myself because I just poured and stored plates in the fridge expecting to use them within the next few days/weeks.
Also, some of the plates had condensation on them so curious to see how to remove that or what not to avoid what the OP had happen. Thoughts?
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Gan
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Re: Storing agar petri dishes in the refrigerator question [Re: Nj.Diesel] 1
#26677877 - 05/17/20 10:35 PM (3 years, 8 months ago) |
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First off, yeah OP if you're not storing your plates for a long time, they're fine to just leave out at room temp. But yes, if you throw plates with condensation in the fridge it'll just drop onto the agar surface. It's a bitch. If they're no pours, you can just pour out the water in your SAB/in front of flow hood.
Only plates I store in the fridge are cultures I wanna keep. If that's what you're talking about, then yeah I take em outta the fridge at least a day before I make transfers off of them. But that's not necessary afaik. It's just something I do
Diesel, if you're not moving your plates or disturbing them at all, I've never had problems with not wrapping plates up immediately. Occassionally, I'll pour a sleeve or two and leave them in my SAB with the holes covered for a day or two with no problems. Only when I get busy with other shit. If I'm moving them or storing them on a shelf, I always wrap them up in the sleeve they come in. Also, place a warm/hot cup of water on top of plates with condensation to clear it up. To prevent it, pour your agar at the coolest temperature possible before it solidifies. I pour mine at around 120-125 °F (~49-52 °C). I think agar begins to solidify around 117 °F.
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NightPuma1
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Re: Storing agar petri dishes in the refrigerator question [Re: Gan] 2
#26677976 - 05/18/20 12:32 AM (3 years, 8 months ago) |
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So basically do not put any agar plates into the refrigerator that have condensation. Is that more or less the jist of it?
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genesis128
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Re: Storing agar petri dishes in the refrigerator question [Re: NightPuma1] 1
#26678064 - 05/18/20 02:10 AM (3 years, 8 months ago) |
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Condensation doesnt hurt. But yeah id only store plates in the fridge if im trying to slow myc growth l. Condensqtion happens more in the fridge.
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poisoned
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Re: Storing agar petri dishes in the refrigerator question [Re: genesis128] 1
#26678085 - 05/18/20 02:45 AM (3 years, 8 months ago) |
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You don't need to put poured dishes into fridge for storage. Just put them in a ziplock back to prevent drying.
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SFS96
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Re: Storing agar petri dishes in the refrigerator question [Re: genesis128] 1
#26678089 - 05/18/20 02:50 AM (3 years, 8 months ago) |
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You can keep empty agar dishes at room temperature for months if u want. No need to put them in the fridge unless your trying to stall the growth on a colonizing dish.
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mushpunx
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Re: Storing agar petri dishes in the refrigerator question [Re: SFS96] 2
#26678137 - 05/18/20 03:49 AM (3 years, 8 months ago) |
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I only put pitres in the fridge when I want to stall the growth (I grow my plates about 3/4 of the way.). With inoculated plates, I want them at room temp so I know they are clean and nothing is going to grow on them.
Temp swings in fridge is common though, storing plates in insulated bags (think lunch box) can make a difference. You might have to fiddle with the temperature too. I had a mini fridge I was using to store plates ruin a bunch, the top of the stack froze, other plates were so full of water the agar turned to mush and my culture was drowned. It took quite a bit of playing around before I got it to work.
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rumfor69
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Re: Storing agar petri dishes in the refrigerator question [Re: mushpunx] 2
#26678167 - 05/18/20 04:26 AM (3 years, 8 months ago) |
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I don't even put dishes in the fridge to stall growth anymore I just let them keep growing so they hopefully develop an in vitro pin I can clone.
I'd only put slants in the fridge for long term storage.
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Nj.Diesel
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Re: Storing agar petri dishes in the refrigerator question [Re: Gan] 1
#26678493 - 05/18/20 09:02 AM (3 years, 8 months ago) |
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Quote:
Gan said: First off, yeah OP if you're not storing your plates for a long time, they're fine to just leave out at room temp. But yes, if you throw plates with condensation in the fridge it'll just drop onto the agar surface. It's a bitch. If they're no pours, you can just pour out the water in your SAB/in front of flow hood.
Only plates I store in the fridge are cultures I wanna keep. If that's what you're talking about, then yeah I take em outta the fridge at least a day before I make transfers off of them. But that's not necessary afaik. It's just something I do
Diesel, if you're not moving your plates or disturbing them at all, I've never had problems with not wrapping plates up immediately. Occassionally, I'll pour a sleeve or two and leave them in my SAB with the holes covered for a day or two with no problems. Only when I get busy with other shit. If I'm moving them or storing them on a shelf, I always wrap them up in the sleeve they come in. Also, place a warm/hot cup of water on top of plates with condensation to clear it up. To prevent it, pour your agar at the coolest temperature possible before it solidifies. I pour mine at around 120-125 °F (~49-52 °C). I think agar begins to solidify around 117 °F.
Great feedback, thanks!
I pour under 120'F and still always get condensation.. it sucks and is super weird. When you use a hot cup/mug on those plates, do you bring that into the SAB, or do you stack it outside of the SAB?The only time I've done it is wrapping the plates with parafilm (to use like an hour later) to get rid of the condensation but the cup melted some of the film (lol). Not sure what the best route is =\
Either way, in my naivety I stored my agar plates in the fridge while they had condensation ... (whoops). I took them out and am letting them get back to room temperature, but should I stack them upside down so the condensations falls to the lids versus the agar, or just let it drop down to the agar? If I stack upside down, what's the best way to get rid of the water in the SAB, do you open them upside down in the SAB and wipe with an alcohol wipe like someone suggested (and let it dry), or?
Thanks!
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Roger Clemency
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Re: Storing agar petri dishes in the refrigerator question [Re: Nj.Diesel] 1
#26678703 - 05/18/20 10:43 AM (3 years, 8 months ago) |
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You can wipe the outside of plates and put them in SAB, then dump the water if you want. I usually just let it sit and it eventually goes away. I’ve had water fall onto the agar after I’d transferred and it didn’t really hurt anything I just try not to let it get on the myc. If you have a contaminant on that plate though the water will spread it all around.
Try to pour plates a good while in advance so you can just set them in a closet or something in a big ziplock. The top plate sometimes keeps a little bit all the others should be clear by a week or so. I like storing at room temp so that if I messed up my pour I will see that bacteria or whatever show up before I ever need the plate.
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muchrooms
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Re: Storing agar petri dishes in the refrigerator question [Re: Roger Clemency] 1
#26678721 - 05/18/20 10:50 AM (3 years, 8 months ago) |
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Store your petris upside down. Open them upside down and your condensation wont land on the agar.
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Nj.Diesel
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Re: Storing agar petri dishes in the refrigerator question [Re: muchrooms] 1
#26678771 - 05/18/20 11:11 AM (3 years, 8 months ago) |
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Quote:
Roger Clemency said: You can wipe the outside of plates and put them in SAB, then dump the water if you want. I usually just let it sit and it eventually goes away. I’ve had water fall onto the agar after I’d transferred and it didn’t really hurt anything I just try not to let it get on the myc. If you have a contaminant on that plate though the water will spread it all around.
Try to pour plates a good while in advance so you can just set them in a closet or something in a big ziplock. The top plate sometimes keeps a little bit all the others should be clear by a week or so. I like storing at room temp so that if I messed up my pour I will see that bacteria or whatever show up before I ever need the plate.
You mean wipe with alcohol right? Then dump the water into a cloth or bowl, etc. inside the SAB and use the plates as normal, close and re-parafilm?
Are you storing upside down or right-side up? I think you're storing right-side up but wanted to confirm
Quote:
muchrooms said: Store your petris upside down. Open them upside down and your condensation wont land on the agar.
See response above to Roger re: cleaning condensation while inside SAB
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Roger Clemency
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Re: Storing agar petri dishes in the refrigerator question [Re: Nj.Diesel] 2
#26678793 - 05/18/20 11:24 AM (3 years, 8 months ago) |
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If your Petri’s have been stored in a ziplock you don’t even really need to wipe em down but someone said something about wiping that made me think they meant wiping inside the lid which is not a good idea. Some people wipe the outside of dishes and jars and stuff W/ alcohol before loading in the SAB but you can do without it if the stuff is pretty clean.
And yeah I store mine right side up. Sometimes if I hold the plate a certain way I can see little water spots on the agar from where water dehydrated off the puck so it will go away given time as long as it’s not serious water. In that case dumping it out might be best.
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A.k.a
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Re: Storing agar petri dishes in the refrigerator question [Re: Roger Clemency] 1
#26678804 - 05/18/20 11:33 AM (3 years, 8 months ago) |
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I’ve got on average prob 12% humidity in my room and even super thin plates last 3-4 months before starting to get dry.
If you put them in the fridge it helps to keep them in an insulated box like a lunchbox or something.
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Nj.Diesel
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Re: Storing agar petri dishes in the refrigerator question [Re: A.k.a] 1
#26678945 - 05/18/20 12:50 PM (3 years, 8 months ago) |
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Quote:
A.k.a said: I’ve got on average prob 12% humidity in my room and even super thin plates last 3-4 months before starting to get dry.
If you put them in the fridge it helps to keep them in an insulated box like a lunchbox or something.
Regarding condensation though on plates you're expecting to use, how do you deal with it? Or do you just use plates as you normally would and ignore th condensation?
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A.k.a
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Re: Storing agar petri dishes in the refrigerator question [Re: Nj.Diesel] 1
#26679009 - 05/18/20 01:31 PM (3 years, 8 months ago) |
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The insulated box will help stop condensation.
If you need to clear it up to see or whatever just put something warm on top of it. Like a cup of hot water or something.
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Gan
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Re: Storing agar petri dishes in the refrigerator question [Re: Nj.Diesel] 1
#26679994 - 05/18/20 10:34 PM (3 years, 8 months ago) |
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Quote:
Nj.Diesel said:
Regarding condensation though on plates you're expecting to use, how do you deal with it? Or do you just use plates as you normally would and ignore th condensation?
If they're blank plates, I just ignore the condensation as it'll eventually clear up. If it's light condensation, I can normally still get a clear enough view of the culture by adding some back lighting. If it's heavy condensation, I either wait for it to clear up or put a mug of hot water on top. And no, you don't need to go in the SAB, since your petris aren't being opened. Leave your plates all wrapped and literally place a cup with warm water on top of an individual plate.
If stacking your plates to pour, as you should be, only the top couple or so will have condensation.
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