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Invisiblemycorry
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Registered: 06/01/18
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Using Denatured Alcohol for making chloramphenicol stock?
    #26676952 - 05/17/20 01:51 PM (3 years, 8 months ago)

I do a lot of work with wild spores so I recently decided to hunt down some chloramphenicol to make agar for the dirtiest of them. I am trying to make chloramphenicol stock solution but I don't have access to isopropyl alcohol or pure ethyl alcohol. I do however have a container of Pure Denatured Alcohol from Ace hardware. The problem with denatured alcohol it contains methanol(up to 49%) to make it poisonous for human consumption. Methanol is cheaper than ethyl alcohol so I imagine that the cheaper products have a higher concentration.

I am posting here to ask if denatured alcohol would be a viable solution(no pun intended) to my lack of ethyl alcohol problem.

Would the methanol content inhibit spore germination? Would it inhibit mycelium growth?

Would I need to alter the stock solution recipe to make up for the methanol?


Thank you!


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OfflineAlan RockefellerM
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Re: Using Denatured Alcohol for making chloramphenicol stock? [Re: mycorry]
    #26680231 - 05/19/20 03:23 AM (3 years, 8 months ago)

Denatured alcohol is fine for making a stock solution of chloramphenicol.


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OfflineSolipsis
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Re: Using Denatured Alcohol for making chloramphenicol stock? [Re: Alan Rockefeller]
    #26683164 - 05/20/20 11:47 AM (3 years, 8 months ago)

Yeah to expand on it: actually in many cases you can get away with a lot of unusual solutions for making stock solutions when you only add tiny amounts of said stock solution to the end medium.

Then it just doesn't matter if there is alcohol in the stock solution (much less methanol vs ethanol) or if it is relatively alkaline or acidic. The latter (pH) can pose a problem if it's extreme and its always a matter of exactly what sort of order of magnitude proportions we're talking about.

Anyway i also do plant tissue culture and some plant growth regulators used require ppm concentrations and it seems to matter very little what you make the stock solution with if you only add say 100 µL to 400 mL.

I don't know what the chloramphenicol concentration is that is used but I guess this is just a similar story. Sometimes you can just ignore it because the proportion is so incredibly tiny but at other (future) times you can calculate how much ends up in your final medium say of alcohol in general.. to double check that it is not a concentration that has significant influence.


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