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Stipe-n Cap
Registered: 08/04/12
Posts: 7,658
Loc: Canada
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Re: Post Your New Tools Of The Trade. [Re: A.k.a]
#26676606 - 05/17/20 10:24 AM (3 years, 10 months ago) |
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Quote:
A.k.a said: Iβm sure they make the punches in different sizes though.
You're probably right. I think that a small inner diameter (angled/bent) stainless steel straw would likely solve the angle of approach/exposure issue and perhaps slightly improve the biopsy size, but imo the best biopsy tool is a syringe.
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gizmo1
Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Post Your New Tools Of The Trade. [Re: A.k.a] 1
#26676610 - 05/17/20 10:26 AM (3 years, 10 months ago) |
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Yea they make biopsy punches pretty small. Josex poke method is a basically a biopsy punch and that's tiny. As far as having hands over a open dish. I won't do it. Ever. I will turn the plate sideways while punching samples then use normally. I would use it on established clean plates while innoculating grain. Don't care much about the photo ops.
I don't think it's a fad. It's not used much in our hobby but biopsy punches are used in medical and scientific settings. I think. It is however becoming more prevalent in our hobby. Not new though.
-------------------- Trade List πππ 6 hole Mini Monos
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Stipe-n Cap
Registered: 08/04/12
Posts: 7,658
Loc: Canada
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Re: Post Your New Tools Of The Trade. [Re: gizmo1]
#26676614 - 05/17/20 10:30 AM (3 years, 10 months ago) |
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No not new. Just my 2 cents on this current iteration.
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gizmo1
Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Post Your New Tools Of The Trade. [Re: Stipe-n Cap]
#26676638 - 05/17/20 10:49 AM (3 years, 10 months ago) |
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Quote:
p9hu7 said: No not new. Just my 2 cents on this current iteration.
I know broham was just putting out my thoughts on the sit. It's new to me never tried biopsy method including syringe biopsy. Figure I'll give her a shot. I might try to find a actual biopsy bunch though a reusable one not one of the sterile disposable ones. I'm not sure they even make those though.
-------------------- Trade List πππ 6 hole Mini Monos
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Stipe-n Cap
Registered: 08/04/12
Posts: 7,658
Loc: Canada
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Re: Post Your New Tools Of The Trade. [Re: gizmo1]
#26676650 - 05/17/20 10:58 AM (3 years, 10 months ago) |
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The issue with a classic biopsy tool is that the size of the punched biopsy is too small to pick up from the plate with a scalpel, ultimately defeating the purpose.
Surgical biopsy core tools are basically syringes anyways.
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gizmo1
Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Post Your New Tools Of The Trade. [Re: Stipe-n Cap]
#26676680 - 05/17/20 11:11 AM (3 years, 10 months ago) |
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Quote:
p9hu7 said: The issue with a classic biopsy tool is that the size of the punched biopsy is too small to pick up from the plate with a scalpel, ultimately defeating the purpose.
Surgical biopsy core tools are basically syringes anyways.
I see that as more of a solution than the problem. Scalpel is not needed with a actual biopsy punch bit is with a leather punch.
-------------------- Trade List πππ 6 hole Mini Monos
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Stipe-n Cap
Registered: 08/04/12
Posts: 7,658
Loc: Canada
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Re: Post Your New Tools Of The Trade. [Re: gizmo1]
#26676704 - 05/17/20 11:22 AM (3 years, 10 months ago) |
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Yeah that's kind of my point, might as well just use a syringe because it's ultimately the exact same thing.
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gizmo1
Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Post Your New Tools Of The Trade. [Re: Stipe-n Cap]
#26677326 - 05/17/20 05:26 PM (3 years, 10 months ago) |
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Quote:
p9hu7 said: Yeah that's kind of my point, might as well just use a syringe because it's ultimately the exact same thing.
Gotcha
-------------------- Trade List πππ 6 hole Mini Monos
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tedoro
ToadStool Tender
Registered: 02/06/15
Posts: 2,212
Last seen: 7 days, 12 hours
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Re: Post Your New Tools Of The Trade. [Re: gizmo1]
#26677521 - 05/17/20 07:11 PM (3 years, 10 months ago) |
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-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Stipe-n Cap
Registered: 08/04/12
Posts: 7,658
Loc: Canada
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Re: Post Your New Tools Of The Trade. [Re: tedoro] 1
#26677564 - 05/17/20 07:32 PM (3 years, 10 months ago) |
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Time for fresh slants
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Smartattack
C'mon man
Registered: 12/21/18
Posts: 3,778
Loc: A thought
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Re: Post Your New Tools Of The Trade. [Re: tedoro]
#26677571 - 05/17/20 07:36 PM (3 years, 10 months ago) |
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How about some for 30 cents a piece instead of $2.00 a piece?
https://www.usascientific.com/100x25-deep-petri-dish/p/8609-0625
Google is friend.
Quote:
tedoro said: I'm looking for some "deep dish" poly petri dishes. Say... 100mm x25mm deep. Has anyone seen them? I'd love to make some LI with a deep deep pour.
Amazon has 20 for like $39. Thats a bit too steep.
https://www.amazon.com/Petri-Dish-Deep-100-Pack/dp/B005UDJDZU/ref=sr_1_2?crid=G01X1W9F7TPE&dchild=1&keywords=petri+dish%2C+deep%2C+25+x+100mm&qid=1589764043&sprefix=%22deep+dish%22+petri%2Caps%2C282&sr=8-2
-------------------- * Smarts videos * Planet of the APES I'm a fungal white supremacist.
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tedoro
ToadStool Tender
Registered: 02/06/15
Posts: 2,212
Last seen: 7 days, 12 hours
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Re: Post Your New Tools Of The Trade. [Re: Smartattack]
#26677918 - 05/17/20 11:24 PM (3 years, 10 months ago) |
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Thanks boo! You must have al gore on speed dial.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Hobbyist
Citizen
Registered: 08/15/10
Posts: 805
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Re: Post Your New Tools Of The Trade. [Re: A.k.a]
#26680329 - 05/19/20 05:02 AM (3 years, 10 months ago) |
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Quote:
A.k.a said: I will say as time goes on I like smaller wedges more and more just in general. Itβs funny looking at old pictures I can see them get progressively smaller.
Iβm sure they make the punches in different sizes though.
Or you could do an overlapping ven diagram punch and use the middle.
Keep in mind, if you're using your ven diagram approach to limit genetics by taking a smaller sample, you're working against yourself unless you're flaming in between punches.
It's the same problem as you'll have if you do your punches the same way D3 does. He just does all his punches each plate at once with a single flaming of the punch before hand. That means all the punches after the first likely have genetics from the previous punches.. Not necessarily a big deal on known clean plates, but if you're hoping to narrow down genetics on different plates...
-------------------- Everything i say is completely hypothetical...
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LogicaL Chaos
Ascension Energy & Alien UFOs
Registered: 05/12/07
Posts: 69,839
Loc: The Inexpressible...
Last seen: 46 minutes, 3 seconds
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Re: Post Your New Tools Of The Trade. [Re: hamloaf]
#26684073 - 05/20/20 07:04 PM (3 years, 10 months ago) |
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I love my new rainbow angled tweezers!
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TedsDead
Registered: 01/03/17
Posts: 5,003
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Re: Post Your New Tools Of The Trade. [Re: LogicaL Chaos]
#26684637 - 05/21/20 12:18 AM (3 years, 10 months ago) |
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what do you use those for?
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it! https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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rickyswamps
Bad Apple
Registered: 11/08/18
Posts: 1,192
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Re: Post Your New Tools Of The Trade. [Re: TedsDead]
#26684899 - 05/21/20 05:47 AM (3 years, 10 months ago) |
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LOL those tweezers have flip flop paint.
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filthyknees
no coincidence
Registered: 03/08/13
Posts: 6,283
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Re: Post Your New Tools Of The Trade. [Re: rickyswamps] 2
#26684978 - 05/21/20 06:50 AM (3 years, 10 months ago) |
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Lol...
New filter, new paint
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Hobbit GDF
Deadhead
Registered: 02/14/19
Posts: 3,445
Loc: Terrapin station
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Re: Post Your New Tools Of The Trade. *DELETED* [Re: filthyknees]
#26689072 - 05/22/20 08:26 PM (3 years, 10 months ago) |
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Post deleted by Hobbit GDF
Reason for deletion: Dont want to post
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CocaineBuffet
Stranger
Registered: 08/29/19
Posts: 3,621
Last seen: 9 hours, 25 seconds
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Re: Post Your New Tools Of The Trade. [Re: filthyknees] 1
#26689087 - 05/22/20 08:36 PM (3 years, 10 months ago) |
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Quote:
filthyknees said: Lol...
New filter, new paint
Just when I thought you couldnt get anymore filthy
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Stipe-n Cap
Registered: 08/04/12
Posts: 7,658
Loc: Canada
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Re: Post Your New Tools Of The Trade. [Re: Hobbit GDF]
#26689237 - 05/22/20 09:38 PM (3 years, 10 months ago) |
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Nice little bioreactor, where did you get it from?
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