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Offline3nt
psychonaut in training
I'm a teapot


Registered: 11/07/19
Posts: 15
Last seen: 3 years, 3 months
getting started with agar (am i on the right track?)
    #26676293 - 05/17/20 05:43 AM (3 years, 8 months ago)

finally got my spores :cool:

the other day i ordered some petri dishes w/agar already in them since i don't have a setup yet to make my own, and i wanted to hit the ground running. they're supposedly sterile already.

i dont own a pc but will likely give in and get one. i do have a steamer, though.

here's my current plan:

1. lc > drop of it onto agar (i don't have a loop yet, but figure literally letting some drop onto the agar will work to get started)
2. get clean mycellium > add agar to brf jar (ordered months ago, came sterilized with lids w/injection port -- i'd just pop open the jars in my sab which i have supplies for, and drop in agar with clean mycel)
3. brf cake then later birthed onto a bed of wetted perlite fruiting chamber like this vid series that was recommended here that i watched, or mixed with coco and try the shoebox tek (i also have verm)

in the meantime i'm going to order supplies for making my own agar plates going forward. but i'm going to go ahead and start with these 'pre-sterilized' plates to see how far i get.

anything major i'm missing? i've read through a lot of articles here, but every time i visit i'm overwhelmed by the abundance of knowledge here lol will take me some time to digest it all.

i think i have a handle on articles to read for agar tek and brf tek and shoebox tek separately, but just making sure they'd all work together.

thanks for reading :rasta: excited to finally get started after months of thinking and learning a bit more each time about how to approach all of this.


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Offlinepoisoned
untitled
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Registered: 04/17/13
Posts: 1,738
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Re: getting started with agar (am i on the right track?) [Re: 3nt]
    #26676302 - 05/17/20 05:58 AM (3 years, 8 months ago)

Brf jars won't work with agar wedges because you have a dry verm layer on top. Unlike grain, they work quite well with spore syringes tho.

Where are you sourcing your LC from? Or is it a spore syringe?

I still recommend you to do agar. You can start the path to enlightenment with grains then.

I didn't understand 100%, did you order pre-made BRF jar? It's unlikely to work after months.


--------------------
How I do glass dishes


Edited by poisoned (05/17/20 05:59 AM)


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OfflinePiggyPig
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Registered: 03/18/20
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Re: getting started with agar (am i on the right track?) [Re: 3nt]
    #26676303 - 05/17/20 06:03 AM (3 years, 8 months ago)

If your plan is to use the PF tek, BRF jars are the way to go. If you plan to spawn to bulk in a shoebox, you're going to want to transfer your agar onto prepared grain. BRF jars are meant to be birthed and fruited, not spawned to coir and verm.

It takes multiple a2a transfers to get the culture cleaned up and get that nice rhizo growth. What I would do in this case to keep the ball rolling is to transfer a couple tiny pieces from the outer edge of the myc on agar and transfer them over to the next plates. Cut the rest of the myc into 6-8 wedges like a pizza and place one in each grain jar. These jars can be used as mothers for g2g or spawned to bulk with coir/verm/gyp.

Don't get your hopes too high for your grain spawn until the culture gets transferred enough times to be clean and genetic content reduced. Every time you repeat this process, your cultures would improve. Once you get the ball rolling, keep up your agar work to keep new, clean cultures coming in every now and then and also look into cloning some of your favourite fruits.

The trick IMO is to always be cultivating (ABC) so when something doesn't go to plan, you're not back at square one. Get some nice clean cultures and look into preserving them on slants for longterm storage. You'll be glad you did. Make sure you figure out a labelling method that is fool proof. Once you get the results you're looking for, you'll be able to follow your labelling back to the source or at least close to it and hopefully make the magic happen over and over and over and over...


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Offline3nt
psychonaut in training
I'm a teapot


Registered: 11/07/19
Posts: 15
Last seen: 3 years, 3 months
Re: getting started with agar (am i on the right track?) [Re: PiggyPig]
    #26676311 - 05/17/20 06:14 AM (3 years, 8 months ago)

Quote:

poisoned said:
Brf jars won't work with agar wedges because you have a dry verm layer on top. Unlike grain, they work quite well with spore syringes tho.

Where are you sourcing your LC from? Or is it a spore syringe?

I still recommend you to do agar. You can start the path to enlightenment with grains then.

I didn't understand 100%, did you order pre-made BRF jar? It's unlikely to work after months.




ohh i see. it's a spore syringe that i have.

yep it was pre-made. good to know...

haha yeah i'm definitely tied to trying agar first.

Quote:

PiggyPig said:
If your plan is to use the PF tek, BRF jars are the way to go. If you plan to spawn to bulk in a shoebox, you're going to want to transfer your agar onto prepared grain. BRF jars are meant to be birthed and fruited, not spawned to coir and verm.

It takes multiple a2a transfers to get the culture cleaned up and get that nice rhizo growth. What I would do in this case to keep the ball rolling is to transfer a couple tiny pieces from the outer edge of the myc on agar and transfer them over to the next plates. Cut the rest of the myc into 6-8 wedges like a pizza and place one in each grain jar. These jars can be used as mothers for g2g or spawned to bulk with coir/verm/gyp.

Don't get your hopes too high for your grain spawn until the culture gets transferred enough times to be clean and genetic content reduced. Every time you repeat this process, your cultures would improve. Once you get the ball rolling, keep up your agar work to keep new, clean cultures coming in every now and then and also look into cloning some of your favourite fruits.

The trick IMO is to always be cultivating (ABC) so when something doesn't go to plan, you're not back at square one. Get some nice clean cultures and look into preserving them on slants for longterm storage. You'll be glad you did. Make sure you figure out a labelling method that is fool proof. Once you get the results you're looking for, you'll be able to follow your labelling back to the source or at least close to it and hopefully make the magic happen over and over and over and over...




thank you so much for the suggestions that sounds like a really good idea (especially having a setup with 'mothers' makes a ton of sense). abc is a good approach i will keep that in mind! i'd like to be really organized to start to make it easier on myself going forward/make my spores last.

thank you both for the super fast detailed responses!! i will start researching prepared grains.


Edited by 3nt (05/17/20 06:15 AM)


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