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OfflineNightPuma1
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Agar question
    #26675555 - 05/16/20 07:31 PM (3 years, 8 months ago)

When you guys take a slice (or slices) of healthy mycelium from a petri dish and transfer it to a new dish, do you always trash the initial petri dish?

It seems to me that leaving that petri dish open while sticking your scalpel in and out of your SAB to flame sterilize it in between transfers is just asking for it to get a contaminant no?

Thanks!


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InvisibleSmartattack
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Re: Agar question [Re: NightPuma1]
    #26675569 - 05/16/20 07:37 PM (3 years, 8 months ago)

Every opening is another chance to contaminate as you say. But by the same logic you should always throw away your new dish as soon as you transfer as well . I always keep plates until Im SURE I dont need that plate anymore. Also, once your technique is good and or you start using a flowhood, these concerns diminish a lot.


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InvisibleRoger Clemency
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Re: Agar question [Re: Smartattack] * 1
    #26675606 - 05/16/20 07:54 PM (3 years, 8 months ago)

I don’t leave my Petri lid off while I’m flaming the blade, I don’t know if anyone does...it’s weird doing something that no one you know in real life does. I lift the lid with my right hand, cut a wedge with my left, and put the lid back on as I’m going towards the grain jar or other dish, once everything is closed up I come out to re sterilize and repeat.

I save old plates for a while and I never see contaminants really. Doesn’t mean they don’t land somewhere in there and just never get to germinate though


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Offlinepoisoned
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Re: Agar question [Re: Roger Clemency]
    #26676216 - 05/17/20 04:24 AM (3 years, 8 months ago)

You shouldn't really leave it open while you sterilize. This is the procedure:
  • Flame the scalpel
  • Open receiving dish
  • Cool the scalpel
  • Close receiving dish
  • Open master dish
  • Take sample
  • Close master dish
  • Open receiving dish
  • Put the sample in
  • Close receiving dish


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OfflineNightPuma1
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Re: Agar question [Re: poisoned]
    #26677387 - 05/17/20 06:06 PM (3 years, 8 months ago)

Thank you.

Wait why not cool the scalpel in the master?


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Offlinebootedboy
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Re: Agar question [Re: NightPuma1] * 1
    #26677407 - 05/17/20 06:19 PM (3 years, 8 months ago)

Quote:

NightPuma1 said:
Thank you.

Wait why not cool the scalpel in the master?




Harms the mycelium, could be done in a sector that you don't plan to use though.


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