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Offlinemarvins
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load syringe from behind
    #26675414 - 05/16/20 06:04 PM (3 years, 8 months ago)



Hello,
i received this LC but the syringe its too short to extract the LC.

Can I load it opening the syringe from behind? Also it helps because the LC is to solid to get through the needle.

Thanks! Trying to grow again since 2015.


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InvisibleFooMan
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Re: load syringe from behind [Re: marvins] * 1
    #26675452 - 05/16/20 06:25 PM (3 years, 8 months ago)

What kinds of tops are on those vials? Are you opening them to extract the LC?


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Offlinemarvins
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Re: load syringe from behind [Re: FooMan]
    #26675671 - 05/16/20 08:27 PM (3 years, 8 months ago)

Quote:

FooMan said:
What kinds of tops are on those vials? Are you opening them to extract the LC?




It has a plastic lid, yes i have to open it.


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Invisiblemushhead
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Re: load syringe from behind [Re: marvins] * 1
    #26675691 - 05/16/20 08:38 PM (3 years, 8 months ago)

Are you using an SAB or flowhood?


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Offlinemarvins
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Re: load syringe from behind [Re: mushhead]
    #26675694 - 05/16/20 08:41 PM (3 years, 8 months ago)

Quote:

mushhead said:
Are you using an SAB or flowhood?




SAB


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Invisiblemushhead
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Re: load syringe from behind [Re: marvins] * 1
    #26675697 - 05/16/20 08:43 PM (3 years, 8 months ago)

What say you fooman? I would say that pouring the LC into the syringe in an SAB wouldn't be too big of a risk. Given proper cleanliness was kept.
of course pouring the LC directly into a jar is another idea you could play with :shrug:


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Offlinemarvins
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Re: load syringe from behind [Re: mushhead]
    #26675705 - 05/16/20 08:49 PM (3 years, 8 months ago)

Quote:

mushhead said:
What say you fooman? I would say that pouring the LC into the syringe in an SAB wouldn't be too big of a risk. Given proper cleanliness was kept.
of course pouring the LC directly into a jar is another idea you could play with :shrug:




Will try with grains on monday but was starting with this grain verm casing kit so I need the needle to go deep.

Will sure try more teks this week, but I still have the "short needle" problem.

thanks for the answers!


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OnlineLogicaL ChaosM
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Re: load syringe from behind [Re: marvins] * 1
    #26675762 - 05/16/20 09:33 PM (3 years, 8 months ago)

Use a clean petri dish as a transfer dish between the vial and the syringe. Or a clean PF jar.


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Offlinemarvins
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Re: load syringe from behind [Re: LogicaL Chaos] * 1
    #26675767 - 05/16/20 09:40 PM (3 years, 8 months ago)

Will try on the pf jar, so loading it from the back of the syringe isnt a good idea haha thanks. Still inoculated that 2 plates, will see what happen in a week.


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Offlinebootedboy
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Re: load syringe from behind [Re: marvins] * 1
    #26675788 - 05/16/20 09:54 PM (3 years, 8 months ago)

Use a clean shot glass, or simply tilt the vials a bit so the needle reaches. Another option is go to a farm supply store and buy longer lower gauge needles.


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InvisibleModularMind
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Re: load syringe from behind [Re: marvins]
    #26675800 - 05/16/20 10:03 PM (3 years, 8 months ago)

Sterile swab to agar. You trust this culture to be contaminant free?


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Offlinemarvins
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Re: load syringe from behind [Re: ModularMind]
    #26675865 - 05/16/20 10:57 PM (3 years, 8 months ago)

Quote:

bootedboy said:
Use a clean shot glass, or simply tilt the vials a bit so the needle reaches. Another option is go to a farm supply store and buy longer lower gauge needles.




Yes i will look for longer needles, if I use a shot glass, wipe it with iso? or PC it?

Quote:

ModularMind said:
Sterile swab to agar. You trust this culture to be contaminant free?




I hope is a good culture, bought it, im really a newbie so nothing of agar for now.

But a store here sells sterilized agar plates with an antibiotic do you think its worth?

they sell them 5 for 18$ and 13$ without the antibiotic.


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InvisibleModularMind
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Re: load syringe from behind [Re: marvins]
    #26675889 - 05/16/20 11:27 PM (3 years, 8 months ago)

It certainly wouldn’t hurt to have them at your disposal.


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Invisiblemushhead
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Re: load syringe from behind [Re: ModularMind]
    #26675920 - 05/17/20 12:05 AM (3 years, 8 months ago)

anti-biotic agar is sometimes not meant for edible cultivation just fyi.


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OnlineLogicaL ChaosM
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Re: load syringe from behind [Re: marvins]
    #26675967 - 05/17/20 12:26 AM (3 years, 8 months ago)

Quote:

marvins said:
Will try on the pf jar, so loading it from the back of the syringe isnt a good idea haha thanks. Still inoculated that 2 plates, will see what happen in a week.




U can load it from behind (giggity) but its a pain in the ass (double giggity). The easier way is a transfer using a jar or Petri dish.


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Offlinebootedboy
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Re: load syringe from behind [Re: marvins]
    #26676378 - 05/17/20 07:13 AM (3 years, 8 months ago)

wash the shot glass with warm soapy water then wipe with iso inside your SAB


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